Methods for enhancing by products from fermentation processes

Abstract

The present invention relates to methods for enhancing yield and/or quality of by-products from fermentation processes used for producing fermentation products. The invention includes a process of fermenting starch-containing material into a fermentation product comprising a fermentation step wherein one or more enzymes are added before or during the fermentation step. More specifically, the present invention provides methods for increasing oil recovery yield from processes for producing fermentation products and further enhancing the quality of DDGS by removing fat content from DDGS that are produced as by-products in processes for producing fermentation products.

1 . A process of dewatering a whole stillage, comprising
 (a) converting a starch-containing material into dextrins with an alpha-amylase;   (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar;   (c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism, wherein the fermentation medium comprises a hemicellulase(s), an endoglucanase(s), and a GH61 polypeptide(s);   (d) distilling the fermentation product to form the whole stillage; and   (e) separating the whole stillage into thin stillage and wet cake, whereby more liquid phase is transferred to the thin stillage.   
     
     
         2 . The process of  claim 1 , wherein the hemicellulase(s) is selected from the group consisting of acetylxylan esterase, arabinofuranosidase, feruloyl esterase, glucuronidase, xylanase, and xylosidase. 
     
     
         3 . The process of  claim 2 , wherein the hemicellulase(s) is a xylanase. 
     
     
         4 . The process of  claim 1 , wherein the fermentation medium further comprises a cellobiohydrolase(s). 
     
     
         5 . The process of  claim 1 , wherein the fermentation medium further comprises a beta-glucosidase(s). 
     
     
         6 . The process of  claim 1 , wherein the fermentation medium further comprises a protease(s). 
     
     
         7 . The process of  claim 1 , wherein the fermentation medium further comprises a GH61 polypeptide(s). 
     
     
         8 . The process of  claim 1 , wherein the fermentation medium further comprises a beta-glucanase(s). 
     
     
         9 . The process of  claim 1 , wherein the fermentation medium further comprises a ferulic acid esterase(s).