Novel methods and devices to stimulate the follicular niche using adipose derived regenerative cells and adipose tissue

Abstract

The present invention concerns a cell-enriched fat graft and methods of making and using the same. The present invention also provides a kit for making and using the cell-enriched fat graft of invention.

We claim: 
     
         1 . A kit for providing a cell-enriched fat graft in situ, which kit comprising an adipose stem and regenerative isolation device; and an adipose tissue cleaning device, wherein the adipose stem and regenerative cells (ADRCs) isolation device comprises a mechanism to enzymatically extract ADRCs from an adipose tissue, and wherein the adipose tissue cleaning device comprises a mechanism to collect and clean an adipose tissue without causing injury to the adipose tissue. 
     
     
         2 . The kit of  claim 1 , wherein the adipose tissue cleaning device comprises a soft bag comprising soft compartments. 
     
     
         3 . A cell-enriched fat graft for hair growth or cilia restoration or prevention of hair loss or cilia loss, comprising a) adipose stem and regenerative cells (ADRCs) and a fat tissue, or b) a platelet rich plasma and a fat tissue, wherein 1 gram of the cell-enriched fat graft comprises: ADRCs in a number ranging from about 10K cells to about 5 million cells, or the platelet rich plasma in a volume ranging from 0.001 mL to 0.9 mL. 
     
     
         4 . The fat graft of  claim 3 , wherein the ADRCs and the fat tissue are provided in situ, and the cell-enriched fat graft is generated by mixing in-situ an amount of the ADRCs and an amount of the fat tissue prior to use. 
     
     
         5 . The fat graft of  claim 4 , wherein the ADRCs are enzymatically extracted in situ from an adipose tissue, and the fat tissue is cleaned in situ. 
     
     
         6 . The fat graft of  claim 4 , wherein the ADRCs are enzymatically extracted in situ and the fat tissue is collected and cleaned in situ from a liposuctioned tissue. 
     
     
         7 . The fat graft of  claim 4 , wherein 1 mL of the cell-enriched fat graft comprises: about 0.1 mL to about 0.9 mL the ADRCs and about 0.9 mL to about 0.1 mL fat tissue, or about 0.1 mL to about 0.9 mL the platelet rich plasma and about 0.9 mL to about 0.1 mL fat tissue. 
     
     
         8 . The fat graft of  claim 5 , wherein 1 mL of the cell-enriched fat graft comprises: about 0.1 mL to about 0.9 mL the ADRCs and about 0.9 mL to about 0.1 mL fat tissue, or about 0.1 mL to about 0.9 mL the platelet rich plasma and about 0.9 mL to about 0.1 mL fat tissue.