Methods for producing and using rejuvenated red blood cells

Abstract

Methods for treating blood. The methods comprise obtaining whole blood from a donor, mixing the whole blood with a red blood cell enhancement composition, and incubating the mixture of whole blood and red blood cell enhancement composition. The method can be performed at a time proximate to the time of obtaining blood from the donor, or after the blood has been stored for a period of time. The method rejuvenates red blood cells.

1 .- 27 . (canceled) 
     
     
         28 . A method of processing a blood component, the method comprising:
 adding a red blood cell (RBC) enhancement composition comprising inosine, adenine, pyruvate, a phosphate mixture, or a combination thereof, to a blood component comprising RBCs;   incubating the blood component and the RBC enhancement composition for a period between about 30 minutes and 90 minutes to form a treated RBC composition; and   measuring the pO 2  at 50% oxygen saturation (p50) of the treated RBC composition during the period.   
     
     
         29 . The method of  claim 28 , wherein the RBC enhancement composition comprises about 25 g/L to about 30 g/L inosine, about 0.2 g/L to about 2 g/L adenine, about 5 g/L to about 15 g/L pyruvate, and about 17 g/L to about 23 g/L sodium phosphate, or a combination thereof. 
     
     
         30 . The method of  claim 28 , wherein the RBC enhancement composition comprises about 27 g/L inosine, about 11 g/L pyruvate, about 0.7 g/L adenine, and about 21 g/L sodium phosphate. 
     
     
         31 . The method of  claim 30 , wherein the sodium phosphate comprises about 6.2 g/L sodium phosphate, monobasic, monohydrate; and about 14.6 g/L sodium phosphate, dibasic, heptahydrate. 
     
     
         32 . The method of  claim 28 , wherein the incubating is at a temperature between 1° C. and about 45° C. 
     
     
         33 . The method of  claim 28 , wherein the incubating is not in a water bath. 
     
     
         34 . The method of  claim 28 , wherein the blood component and the RBC enhancement composition are mixed during the period. 
     
     
         35 . The method of  claim 28 , wherein the p50 of the treated RBC composition is greater than or equal to the p50 of fresh blood. 
     
     
         36 . A method of transfusing a blood component, the method comprising:
 adding a red blood cell (RBC) enhancement composition comprising inosine, adenine, pyruvate, a phosphate mixture, or a combination thereof, to a blood component comprising RBCs;   incubating the blood component and the RBC enhancement composition for a period between about 30 minutes and 90 minutes to form a treated RBC composition;   measuring the pO 2  at 50% oxygen saturation (p50) of the treated RBC composition during the period; and   administering the treated RBC composition to a subject if the p50 of the treated RBC composition is at least equal to the p50 of fresh blood.   
     
     
         37 . The method of  claim 36 , further comprising testing the p50 of the blood component for before adding the RBC enhancement composition. 
     
     
         38 . The method of  claim 36 , wherein the blood component is autologous to the subject. 
     
     
         39 . The method of  claim 36 , the blood component is leukoreduced. 
     
     
         40 . The method of  claim 36 , wherein the RBC enhancement composition comprises about 25 g/L to about 30 g/L inosine, about 0.2 g/L to about 2 g/L adenine, about 5 g/L to about 15 g/L pyruvate, and about 17 g/L to about 23 g/L sodium phosphate, or a combination thereof. 
     
     
         41 . The method of  claim 36 , wherein the RBC enhancement composition comprises about 27 g/L inosine, about 11 g/L pyruvate, about 0.7 g/L adenine, and about 21 g/L sodium phosphate. 
     
     
         42 . The method of  claim 41 , wherein the sodium phosphate comprises about 6.2 g/L sodium phosphate, monobasic, monohydrate; and about 14.6 g/L sodium phosphate, dibasic, heptahydrate. 
     
     
         43 . The method of  claim 36 , wherein the incubating is at a temperature between 1° C. and about 45° C. 
     
     
         44 . The method of  claim 36 , wherein the incubating is not in a water bath. 
     
     
         45 . The method of  claim 36 , wherein the blood component and the RBC enhancement composition are mixed during the period. 
     
     
         46 . The method of  claim 36 , wherein the p50 of the treated RBC composition is greater than the p50 of fresh blood. 
     
     
         47 . The method of  claim 36 , wherein the treated RBC composition is stored at a temperature between −80° C. and about 6° C. before administering to the subject.