Anti-thrombogenic grafts

Abstract

The present invention provides anti-thrombogenic compositions, including anti-thrombogenic vascular grafts. In certain embodiments, the compositions comprise decellularized tissue coated with an anti-thrombogenic coating. The present invention also provides methods of preparing anti-thrombogenic compositions and methods of treatment comprising implanting the anti-thrombogenic compositions into a subject in need thereof.

What is claimed: 
     
         1 . A composition comprising a substrate having at least one surface coated with an anti-thrombogenic coating comprising thiol-modified hyaluronic acid. 
     
     
         2 . The composition of  claim 1 , wherein the anti-thrombogenic coating is crosslinked to the at least one surface of the substrate. 
     
     
         3 . The composition of  claim 1 , wherein the anti-thrombogenic coating comprises a therapeutic agent. 
     
     
         4 . The composition of  claim 1 , wherein the substrate is decellularized tissue. 
     
     
         5 . The composition of  claim 4 , wherein the decellularized tissue is a decellularized blood vessel having a luminal surface, and wherein the anti-thrombogenic coating is coated on the luminal surface of the decellularized blood vessel. 
     
     
         6 . A method of preparing a graft coated with an anti-thrombogenic coating comprising thiol-modified hyaluronic acid, comprising the steps of:
 providing a substrate having at least one surface; and   coating the at least one surface with an anti-thrombogenic coating, wherein said step of coating comprises:   applying a first crosslinking solution to the surface; and   applying a hydrogel solution to the surface, thereby providing an anti-thrombogenic coating on the surface of the substrate, wherein the hydrogel solution comprises thiol-modified hyaluronic acid.   
     
     
         7 . The method of  claim 6 , wherein the substrate is decellularized tissue. 
     
     
         8 . The method of  claim 7 , wherein the decellularized tissue is a decellularized blood vessel. 
     
     
         9 . A method of treating a diseased blood vessel in a subject, comprising bypassing the diseased blood vessel by implanting into the subject an anti-thrombogenic vascular graft, comprising a substrate having a luminal surface coated with an anti-thrombogenic coating comprising thiol-modified hyaluronic acid. 
     
     
         10 . The method of  claim 9 , wherein the subject has a disorder selected from the group consisting of peripheral vascular disease, atherosclerosis, aneurysm, and venous thrombosis. 
     
     
         11 . The method of  claim 9 , wherein the anti-thrombogenic coating is crosslinked to the luminal surface of the substrate. 
     
     
         12 . The method of  claim 9 , wherein the substrate is a decellularized blood vessel. 
     
     
         13 . A method of providing vascular access in a subject, comprising implanting into the subject an anti-thrombogenic vascular graft, comprising a substrate having a luminal surface coated with an anti-thrombogenic coating comprising thiol-modified hyaluronic acid. 
     
     
         14 . The method of  claim 13 , wherein the subject is undergoing or is anticipated to undergo hemodialysis. 
     
     
         15 . The method of  claim 13 , wherein the anti-thrombogenic coating is crosslinked to the luminal surface of the substrate. 
     
     
         16 . The method of  claim 13 , wherein the substrate is a decellularized blood vessel.