US2018280422A1PendingUtilityA1

Substituted purine and 7-deazapurine compounds

61
Assignee: EPIZYME INCPriority: Dec 3, 2010Filed: Nov 3, 2017Published: Oct 4, 2018
Est. expiryDec 3, 2030(~4.4 yrs left)· nominal 20-yr term from priority
A61P 9/00A61P 43/00A61P 35/00A61P 35/02A61P 25/28A61P 29/00A61P 3/00A61K 31/52A61K 31/7076C07H 19/14A61K 31/7064C07D 487/04C07H 19/04A61K 31/519C07D 473/34C07H 19/167C07H 19/16A61P 25/00
61
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Claims

Abstract

The present invention relates to substituted purine and 7-deazapurine compounds. The present invention also relates to pharmaceutical compositions containing these compounds and methods of treating disorders in which DOT1-mediated protein methylation plays a part, such as cancer and neurological disorders, by administering these compounds and pharmaceutical compositions to subjects in need thereof.

Claims

exact text as granted — not AI-modified
1 . (canceled) 
     
     
         2 . A method of treating cancer comprising administering to a subject in need thereof a therapeutically effective amount of a DOT1L inhibitor. 
     
     
         3 . A method of treating hematological cancer comprising administering to a subject in need thereof a therapeutically effective amount of a DOT1L inhibitor. 
     
     
         4 . A method of treating leukemia comprising administering to a subject in need thereof a therapeutically effective amount of a DOT1L inhibitor. 
     
     
         5 . The method of  claim 4 , wherein the leukemia is acute myeloid leukemia, acute lymphocytic leukemia or mixed lineage leukemia. 
     
     
         6 . The method of  claim 4 , wherein the leukemia is a MLL-rearranged leukemia. 
     
     
         7 . The method of  claim 4 , wherein the leukemia is a leukemia characterized by a partial tandem duplication of the MLL gene (MLL-PTD). 
     
     
         8 . The method of  claim 2 , wherein the DOT1L inhibitor is a compound having the structure of Formula (II): 
       
         
           
           
               
               
           
         
         or a pharmaceutically acceptable salt thereof, wherein
 A is O or CH 2 ; 
 Q is H, NH 2 , NHR b , NR b R c , R b , or OR b , in which each of R b  and R c  independently is C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 7-membered heterocycloalkyl, 5 to 10-membered heteroaryl, or -M 1 -T 1  in which M 1  is a bond or C 1 -C 6  alkyl linker optionally substituted with halo, cyano, hydroxyl or C 1 -C 6  alkoxyl and T 1  is C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 10-membered heteroaryl, or R b  and R c , together with the N atom to which they attach, form 4 to 7-membered heterocycloalkyl having 0 or 1 additional heteroatoms to the N atom optionally substituted with C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, halo, hydroxyl, carboxyl, C(O)OH, C(O)O—C 1 -C 6  alkyl, OC(O)—C 1 -C 6  alkyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl, and each of R b , R c , and T 1  is optionally substituted with one or more substituents selected from the group consisting of C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl; 
 X is N or CR x , in which R x  is H, halo, hydroxyl, carboxyl, cyano, or R S1 , R S1  being amino, C 1 -C 6  alkoxyl, C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl, and R S1  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl; 
 L 1  is N(Y), S, SO, or SO 2 ; 
 L 2  is CO or absent when L 1  is N(Y) or L 2  is absent when L 1  is S, SO, or SO 2 , in which Y is H, R d , SO 2 R d , or COR d  when L 2  is absent, or Y is H or R d  when L 2  is CO, R d  being C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl, and R d  being optionally substituted with one or more substituents selected from the group consisting of C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, C 1 -C 6  alkylsulfonyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl and with C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl further optionally substituted with C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, halo, hydroxyl, carboxyl, C(O)OH, C(O)O—C 1 -C 6  alkyl, OC(O)—C 1 -C 6  alkyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl; 
 each of R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , and R 7 , independently, is H, halo, hydroxyl, carboxyl, cyano, R S2 , R S2  being amino, C 1 -C 6  alkoxyl, C 1 -C 6  alkyl, C 2 -C 6  alkenyl, or C 2 -C 6  alkynyl, and each R S2  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl; 
 R 8  is H, halo or R S3 , R S3  being C 1 -C 6  alkyl, C 2 -C 6  alkenyl, or C 2 -C 6  alkynyl, and R S3  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano amino, C 1 -C 6  alkoxyl, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, and C 3 -C 8  cycloalkyl; 
 R 9  is 
 
       
       
         
           
           
               
               
           
         
       
       in which each of R e , R f , R g , and R h , independently is -M 2 -T 2 , in which M 2  is a bond, SO 2 , SO, S, CO, CO 2 , O, O—C 1 -C 4  alkyl linker, C 1 -C 4  alkyl linker, NH, or N(R t ), R t  being C 1 -C 6  alkyl, and T 2  is H, halo, or R S4 , R S4  being C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 8-membered heterocycloalkyl, or 5 to 10-membered heteroaryl, and each of O—C 1 -C 4  alkyl linker, C 1 -C 4  alkyl linker, R t , and R S4  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl, R i  is H or C 1 -C 6  alkyl optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl, D is O, NR j , or CR j R k , each of R j  and R k  independently being H or C 1 -C 6  alkyl, or R j  and R k  taken together, with the carbon atom to which they are attached, form a C 3 -C 10  cycloalkyl ring, and E is -M 3 -T 3 , M 3  being a bond or C 1 -C 6  alkyl linker optionally substituted with halo or cyano, T 3  being C 3 -C 10  cycloalkyl, C 6 -C 10  aryl, 5 to 10-membered heteroaryl, or 4 to 10-membered heterocycloalkyl, and T 3  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, thiol, carboxyl, cyano, nitro, C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 1 -C 6  alkoxyl, C 1 -C 6  haloalkyl, C 1 -C 6  haloalkoxyl, C 1 -C 6  alkylthio, C 1 -C 6  alkylsulfonyl, C 1 -C 6  haloalkylsulfonyl, C 1 -C 6  alkylcarbonyl, C 1 -C 6  alkoxycarbonyl, oxo, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 4 -C 12  alkylcycloalkyl, C 6 -C 10  aryl, C 6 -C 10  aryloxyl, C 7 -C 14  alkylaryl, C 6 -C 10  aminoaryloxyl, C 6 -C 10  arylthio, 4 to 6-membered heterocycloalkyl optionally substituted with halo, C 1 -C 4  alkyl, C 1 -C 4  haloalkyl, 5 to 6-membered heteroaryl optionally substituted with halo, C 1 -C 4  alkyl, and C 1 -C 6  alkyl that is substituted with hydroxy, halo, C 1 -C 6  alkoxycarbonyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl optionally further substituted with halo, hydroxyl, or C 1 -C 6  alkoxyl;
   q is 0, 1, 2, 3, or 4;   m is 0, 1, or 2; and   n is 0, 1, or 2.   
 
     
     
         9 . The method of  claim 3 , wherein the DOT1L inhibitor is a compound having the structure of Formula (II): 
       
         
           
           
               
               
           
         
         or a pharmaceutically acceptable salt thereof, wherein
 A is O or CH 2 ; 
 Q is H, NH 2 , NHR b , NR b R c , R b , or OR b , in which each of R b  and R c  independently is C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 7-membered heterocycloalkyl, 5 to 10-membered heteroaryl, or -M 1 -T 1  in which M 1  is a bond or C 1 -C 6  alkyl linker optionally substituted with halo, cyano, hydroxyl or C 1 -C 6  alkoxyl and T 1  is C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 10-membered heteroaryl, or R b  and R c , together with the N atom to which they attach, form 4 to 7-membered heterocycloalkyl having 0 or 1 additional heteroatoms to the N atom optionally substituted with C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, halo, hydroxyl, carboxyl, C(O)OH, C(O)O—C 1 -C 6  alkyl, OC(O)—C 1 -C 6  alkyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl, and each of R b , R c , and T 1  is optionally substituted with one or more substituents selected from the group consisting of C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl; 
 X is N or CR x , in which R x  is H, halo, hydroxyl, carboxyl, cyano, or R S1 , R S1  being amino, C 1 -C 6  alkoxyl, C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl, and R S1  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl; 
 L 1  is N(Y), S, SO, or SO 2 ; 
 L 2  is CO or absent when L 1  is N(Y) or L 2  is absent when L 1  is S, SO, or SO 2 , in which Y is H, R d , SO 2 R d , or COR d  when L 2  is absent, or Y is H or R d  when L 2  is CO, R d  being C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl, and R d  being optionally substituted with one or more substituents selected from the group consisting of C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, C 1 -C 6  alkylsulfonyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl and with C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl further optionally substituted with C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, halo, hydroxyl, carboxyl, C(O)OH, C(O)O—C 1 -C 6  alkyl, OC(O)—C 1 -C 6  alkyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl; 
 each of R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , and R 7 , independently, is H, halo, hydroxyl, carboxyl, cyano, R S2 , R S2  being amino, C 1 -C 6  alkoxyl, C 1 -C 6  alkyl, C 2 -C 6  alkenyl, or C 2 -C 6  alkynyl, and each R S2  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl; 
 R 8  is H, halo or R S3 , R S3  being C 1 -C 6  alkyl, C 2 -C 6  alkenyl, or C 2 -C 6  alkynyl, and R S3  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano amino, C 1 -C 6  alkoxyl, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, and C 3 -C 8  cycloalkyl; 
 R 9  is 
 
       
       
         
           
           
               
               
           
         
       
       in which each of R e , R f , R g , and R h , independently is -M 2 -T 2 , in which M 2  is a bond, SO 2 , SO, S, CO, CO 2 , O, O—C 1 -C 4  alkyl linker, C 1 -C 4  alkyl linker, NH, or N(R t ), R t  being C 1 -C 6  alkyl, and T 2  is H, halo, or R S4 , R S4  being C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 8-membered heterocycloalkyl, or 5 to 10-membered heteroaryl, and each of O—C 1 -C 4  alkyl linker, C 1 -C 4  alkyl linker, R t , and R S4  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl, R i  is H or C 1 -C 6  alkyl optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl, D is O, NR j , or CR j R k , each of R j  and R k  independently being H or C 1 -C 6  alkyl, or R j  and R k  taken together, with the carbon atom to which they are attached, form a C 3 -C 10  cycloalkyl ring, and E is -M 3 -T 3 , M 3  being a bond or C 1 -C 6  alkyl linker optionally substituted with halo or cyano, T 3  being C 3 -C 10  cycloalkyl, C 6 -C 10  aryl, 5 to 10-membered heteroaryl, or 4 to 10-membered heterocycloalkyl, and T 3  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, thiol, carboxyl, cyano, nitro, C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 1 -C 6  alkoxyl, C 1 -C 6  haloalkyl, C 1 -C 6  haloalkoxyl, C 1 -C 6  alkylthio, C 1 -C 6  alkylsulfonyl, C 1 -C 6  haloalkylsulfonyl, C 1 -C 6  alkylcarbonyl, C 1 -C 6  alkoxycarbonyl, oxo, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 4 -C 12  alkylcycloalkyl, C 6 -C 10  aryl, C 6 -C 10  aryloxyl, C 7 -C 14  alkylaryl, C 6 -C 10  aminoaryloxyl, C 6 -C 10  arylthio, 4 to 6-membered heterocycloalkyl optionally substituted with halo, C 1 -C 4  alkyl, C 1 -C 4  haloalkyl, 5 to 6-membered heteroaryl optionally substituted with halo, C 1 -C 4  alkyl, and C 1 -C 6  alkyl that is substituted with hydroxy, halo, C 1 -C 6  alkoxycarbonyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl optionally further substituted with halo, hydroxyl, or C 1 -C 6  alkoxyl;
   q is 0, 1, 2, 3, or 4;   m is 0, 1, or 2; and   
 n is 0, 1, or 2. 
 
     
     
         10 . The method of  claim 4 , wherein the DOT1L inhibitor is a compound having the structure of Formula (II): 
       
         
           
           
               
               
           
         
         or a pharmaceutically acceptable salt thereof, wherein
 A is O or CH 2 ; 
 Q is H, NH 2 , NHR b , NR b R c , R b , or OR b , in which each of R b  and R c  independently is C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 7-membered heterocycloalkyl, 5 to 10-membered heteroaryl, or -M 1 -T 1  in which M 1  is a bond or C 1 -C 6  alkyl linker optionally substituted with halo, cyano, hydroxyl or C 1 -C 6  alkoxyl and T 1  is C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 10-membered heteroaryl, or R b  and R c , together with the N atom to which they attach, form 4 to 7-membered heterocycloalkyl having 0 or 1 additional heteroatoms to the N atom optionally substituted with C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, halo, hydroxyl, carboxyl, C(O)OH, C(O)O—C 1 -C 6  alkyl, OC(O)—C 1 -C 6  alkyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl, and each of R b , R c , and T 1  is optionally substituted with one or more substituents selected from the group consisting of C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl; 
 X is N or CR x , in which R x  is H, halo, hydroxyl, carboxyl, cyano, or R S1 , R S1  being amino, C 1 -C 6  alkoxyl, C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl, and R S1  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl; 
 L 1  is N(Y), S, SO, or SO 2 ; 
 L 2  is CO or absent when L 1  is N(Y) or L 2  is absent when L 1  is S, SO, or SO 2 , in which Y is H, R d , SO 2 R d , or COR d  when L 2  is absent, or Y is H or R d  when L 2  is CO, R d  being C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl, and R d  being optionally substituted with one or more substituents selected from the group consisting of C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, C 1 -C 6  alkylsulfonyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl and with C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl further optionally substituted with C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, halo, hydroxyl, carboxyl, C(O)OH, C(O)O—C 1 -C 6  alkyl, OC(O)—C 1 -C 6  alkyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl; 
 each of R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , and R 7 , independently, is H, halo, hydroxyl, carboxyl, cyano, R S2 , R S2  being amino, C 1 -C 6  alkoxyl, C 1 -C 6  alkyl, C 2 -C 6  alkenyl, or C 2 -C 6  alkynyl, and each R S2  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl; 
 R 8  is H, halo or R S3 , R S3  being C 1 -C 6  alkyl, C 2 -C 6  alkenyl, or C 2 -C 6  alkynyl, and R S3  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano amino, C 1 -C 6  alkoxyl, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, and C 3 -C 8  cycloalkyl; 
 R 9  is 
 
       
       
         
           
           
               
               
           
         
       
       in which each of R e , R f , R g , and R h , independently is -M 2 -T 2 , in which M 2  is a bond, SO 2 , SO, S, CO, CO 2 , O, O—C 1 -C 4  alkyl linker, C 1 -C 4  alkyl linker, NH, or N(R t ), R t  being C 1 -C 6  alkyl, and T 2  is H, halo, or R S4 , R S4  being C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 8-membered heterocycloalkyl, or 5 to 10-membered heteroaryl, and each of O—C 1 -C 4  alkyl linker, C 1 -C 4  alkyl linker, R t , and R S4  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C6-C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl, R i  is H or C 1 -C 6  alkyl optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, carboxyl, cyano, C 1 -C 6  alkoxyl, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, and 5 to 6-membered heteroaryl, D is O, NR j , or CR j R k , each of R j  and R k  independently being H or C 1 -C 6  alkyl, or R j  and R k  taken together, with the carbon atom to which they are attached, form a C 3 -C 10  cycloalkyl ring, and E is -M 3 -T 3 , M 3  being a bond or C 1 -C 6  alkyl linker optionally substituted with halo or cyano, T 3  being C 3 -C 10  cycloalkyl, C 6 -C 10  aryl, 5 to 10-membered heteroaryl, or 4 to 10-membered heterocycloalkyl, and T 3  being optionally substituted with one or more substituents selected from the group consisting of halo, hydroxyl, thiol, carboxyl, cyano, nitro, C 1 -C 6  alkyl, C 2 -C 6  alkenyl, C 2 -C 6  alkynyl, C 1 -C 6  alkoxyl, C 1 -C 6  haloalkyl, C 1 -C 6  haloalkoxyl, C 1 -C 6  alkylthio, C 1 -C 6  alkylsulfonyl, C 1 -C 6  haloalkylsulfonyl, C 1 -C 6  alkylcarbonyl, C 1 -C 6  alkoxycarbonyl, oxo, amino, mono-C 1 -C 6  alkylamino, di-C 1 -C 6  alkylamino, C 3 -C 8  cycloalkyl, C 4 -C 12  alkylcycloalkyl, C 6 -C 10  aryl, C 6 -C 10  aryloxyl, C 7 -C 14  alkylaryl, C 6 -C 10  aminoaryloxyl, C 6 -C 10  arylthio, 4 to 6-membered heterocycloalkyl optionally substituted with halo, C 1 -C 4  alkyl, C 1 -C 4  haloalkyl, 5 to 6-membered heteroaryl optionally substituted with halo, C 1 -C 4  alkyl, and C 1 -C 6  alkyl that is substituted with hydroxy, halo, C 1 -C 6  alkoxycarbonyl, C 3 -C 8  cycloalkyl, C 6 -C 10  aryl, 4 to 6-membered heterocycloalkyl, or 5 to 6-membered heteroaryl optionally further substituted with halo, hydroxyl, or C 1 -C 6  alkoxyl;
   q is 0, 1, 2, 3, or 4;   m is 0, 1, or 2; and   
 n is 0, 1, or 2. 
 
     
     
         11 . The method of  claim 8 , wherein A is O. 
     
     
         12 . The method of  claim 9 , wherein A is O. 
     
     
         13 . The method of  claim 10 , wherein A is O. 
     
     
         14 . The method of  claim 8 , wherein Q is NH 2  or NHR b , in which R b  is -M 1 -T 1 , M 1  being a bond or C 1 -C 6  alkyl linker and T 1  being C 3 -C 8  cycloalkyl. 
     
     
         15 . The method of  claim 9 , wherein Q is NH 2  or NHR b , in which R b  is -M 1 -T 1 , M 1  being a bond or C 1 -C 6  alkyl linker and T 1  being C 3 -C 8  cycloalkyl. 
     
     
         16 . The method of  claim 10 , wherein Q is NH 2  or NHR b , in which R b  is -M 1 -T 1 , M 1  being a bond or C 1 -C 6  alkyl linker and T 1  being C 3 -C 8  cycloalkyl. 
     
     
         17 . The method of  claim 2 , wherein the DOT1L inhibitor is a compound selected from: 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         and pharmaceutically acceptable salts thereof. 
       
     
     
         18 . The method of  claim 3 , wherein the DOT1L inhibitor is a compound selected from: 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         and pharmaceutically acceptable salts thereof. 
       
     
     
         19 . The method of  claim 4 , wherein the DOT1L inhibitor is a compound selected from: 
       
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         
           
           
               
               
           
         
         and pharmaceutically acceptable salts thereof. 
       
     
     
         20 . The method of  claim 2 , wherein the DOT1L inhibitor is Compound 2: 
       
         
           
           
               
               
           
         
         or a pharmaceutically acceptable salt thereof. 
       
     
     
         21 . The method of  claim 3 , wherein the DOT1L inhibitor is Compound 2: 
       
         
           
           
               
               
           
         
         or a pharmaceutically acceptable salt thereof. 
       
     
     
         22 . The method of  claim 4 , wherein the DOT1L inhibitor is Compound 2: 
       
         
           
           
               
               
           
         
         or a pharmaceutically acceptable salt thereof.

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