Production of high quality durum wheat having increased amylose content

Abstract

The present invention provides compositions and methods of altering/improving Durum wheat phenotypes. Furthermore, methods of breeding Durum wheat and/or other closely related species to produce plants having altered or improved phenotypes are provided.

1 - 55 . (canceled) 
     
     
         56 . A method for producing a durum wheat plant comprising one or more mutant durum starch granule protein-B1 (SGP-B1) allele(s), and one or more mutant durum starch granule protein-A1 (SGP-A1) allele(s), the method comprising:
 a. mutagenizing durum wheat grain to produce a mutagenized population of grain, wherein the durum wheat grain comprises a previously existing mutant durum SGP-B1 allele or a previously existing mutant durum SGP-A1 allele prior to mutagenizing, and wherein the previously existing mutant durum SGP-B1 allele and the previously existing mutant durum SGP-A1 allele are not derived from a hexaploid wheat;   b. growing one or more durum wheat plants from said mutagenized durum wheat grain;   c. screening the resulting plants from step (b) for a newly produced mutant durum SGP-B1 allele or a newly produced mutant durum SGP-A1 allele that is located on a different genome relative to the previously existing mutant durum SGP-B1 allele or the previously existing mutant durum SGP-A1 allele of step (a), wherein both the newly produced and previously existing SGP-B1 and SGP-A1 mutant alleles disrupt activity of their encoded proteins as determined by SDS PAGE; and   d. selecting a durum wheat plant comprising the newly produced mutant durum SGP-B1 allele or the newly produced mutant durum SGP-A1 allele;   
       wherein the resulting plant comprises one or more mutant durum SGP-B1 allele(s) and one or more mutant durum SGP-A1 allele(s). 
     
     
         57 . The method of  claim 56 , wherein the durum wheat grain comprises the previously existing mutant durum SGP-A1 allele, and where the previously existing mutant durum SGP-A1 allele of step (a) comprises a 29 bp deletion as found in PI-330546 wheat line. 
     
     
         58 . The method of  claim 56 , wherein the durum wheat grain comprises the previously existing mutant durum SGP-B1 allele, and where the previously existing mutant durum SGP-B1 allele of step (a) encodes an amino acid substitution from aspartic acid to asparagine at an amino acid position corresponding to amino acid 327 of SEQ ID No. 6. 
     
     
         59 . The method of  claim 56 , wherein the durum wheat grain comprises the previously existing mutant durum SGP-B1 allele, and where the previously existing mutant durum SGP-B1 allele of step (a) encodes an amino acid substitution from aspartic acid to asparagine at an amino acid position corresponding to amino acid 622 of SEQ ID No. 6. 
     
     
         60 . The method of  claim 56 , comprising an additional step of crossing the plant from step (d) with itself or with a second plant for one or more generations to produce a durum wheat plant that is homozygous for the mutant SGP-A1 and SGP-B1 alleles. 
     
     
         61 . The method of  claim 60 , wherein the durum wheat plant that is homozygous for the SGP-A1 and SGP-B1 mutant alleles produces high amylose grain, and wherein the proportion of amylose in the starch of said high amylose grain is greater than about 38% as measured by differential scanning calorimetry analysis. 
     
     
         62 . The method of  claim 60 , wherein the durum wheat plant that is homozygous for the SGP-A1 and SGP-B1 mutant alleles produces high amylose grain, and wherein the proportion of amylose in the starch of said high amylose grain is at least 25% higher than the proportion of amylose in the starch from grain of an appropriate durum wheat reference variety grown under similar field conditions. 
     
     
         63 . The method of  claim 60 , wherein the durum wheat plant that is homozygous for the SGP-A1 and SGP-B1 mutant alleles produces high amylose grain, and wherein the proportion of amylose in the starch of said high amylose grain is at least 35% higher than the proportion of amylose in the starch from grain of an appropriate durum wheat reference variety grown under similar field conditions. 
     
     
         64 . A method of producing a durum wheat plant comprising one or more durum starch granule protein-B1 null (SGP-B1-null) allele(s), and one or more durum starch granule protein-A1 null (SGP-A1-null) allele(s), the method comprising:
 a. crossing a durum wheat plant comprising one or more SGP-A1-null allele(s) with a second durum wheat plant comprising one or more SGP-B1-null allele(s) to produce F1 progeny plants; and   b. selecting for an F1 progeny plant comprising at least one copy of each of the SGP-A1-null and SGP-B1-null alleles;   
       wherein none of the SGP-A1-null or SGP-B1-null alleles are derived from hexaploid wheat. 
     
     
         65 . The method of  claim 64 , wherein at least one SGP-B1-null allele encodes an amino acid substitution from aspartic acid to asparagine at an amino acid position corresponding to amino acid 327 of SEQ ID No. 6. 
     
     
         66 . The method of  claim 64 , wherein at least one SGP-B1-null allele encodes an amino acid substitution from aspartic acid to asparagine at an amino acid position corresponding to amino acid 622 of SEQ ID No. 6. 
     
     
         67 . The method of  claim 64 , wherein at least one SGP-A1-null allele comprises a 29 bp deletion as found in PI-330546 wheat line. 
     
     
         68 . The method of  claim 64 , comprising an additional step of crossing the plant from step (b) with itself or with a second plant for one or more generations to produce a durum wheat plant that is homozygous for the SGP-A1-null and SGP-B1-null alleles. 
     
     
         69 . The method of  claim 68 , wherein the durum wheat plant that is homozygous for the SGP-A1-null and SGP-B1-null alleles produces high amylose grain, and wherein the proportion of amylose in the starch of said high amylose grain is greater than about 38% as measured by differential scanning calorimetry analysis. 
     
     
         70 . The method of  claim 68 , wherein the durum wheat plant that is homozygous for the SGP-A1-null and SGP-B1-null alleles produces high amylose grain, and wherein the proportion of amylose in the starch of said high amylose grain is at least 25% higher than the proportion of amylose in the starch from grain of an appropriate durum wheat reference variety grown under similar field conditions. 
     
     
         71 . The method of  claim 68 , wherein the durum wheat plant that is homozygous for the SGP-A1-null and SGP-B1-null alleles produces high amylose grain, and wherein the proportion of amylose in the starch of said high amylose grain is at least 35% higher than the proportion of amylose in the starch from grain of an appropriate durum wheat reference variety grown under similar field conditions. 
     
     
         72 . A method for producing a durum wheat plant comprising one or more mutant durum starch granule protein-B1 (SGP-B1) allele(s), and one or more mutant durum starch granule protein-A1 (SGP-A1) allele(s), the method comprising:
 a. mutagenizing durum wheat grain to produce a mutagenized population of grain;   b. growing one or more durum wheat plants from said mutagenized durum wheat grain;   c. screening the resulting plants from step (b) for a mutant durum SGP-B1 allele and a mutant durum SGP-A1 allele wherein the mutant durum SGP-B1 allele and the mutant durum SGP-A1 mutant alleles disrupt activity of their encoded proteins as determined by SDS PAGE; and   selecting one or more durum wheat plants, wherein each selected plant comprises a mutant durum SGP-B1 allele and a mutant durum SGP-A1 allele.