Polypeptides Having Xylanase Activity And Polynucleotides Thereof

Abstract

The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

1 - 60 . (canceled) 
     
     
         61 . An isolated recombinant host cell transformed with a nucleic acid construct comprising an isolated polynucleotide encoding a polypeptide having xylanase activity operably linked to one or more control sequences that direct production of the polypeptide in the recombinant host cell, wherein the polypeptide having xylanase activity is heterologous to the recombinant host cell and wherein the polynucleotide is selected from the group consisting of:
 (a) a polynucleotide encoding a polypeptide having xylanase activity comprising an amino acid sequence having at least 95% identity to amino acids 18 to 364 of SEQ ID NO: 2;   (b) a polynucleotide encoding a polypeptide having xylanase activity which hybridizes under high stringency conditions with (i) nucleotides 52 to 1145 of SEQ ID NO: 1, (ii) the cDNA sequence of nucleotides 52 to 1145 of SEQ ID NO: 1, or (iii) the full-length complement of (i) or (ii), wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes in 2×SSC, 0.2% SDS at 65° C.;   (c) a polynucleotide encoding a polypeptide having xylanase activity comprising a polynucleotide sequence having at least 95% identity to nucleotides 52 to 1145 of SEQ ID NO: 1;   (d) a polynucleotide encoding a polypeptide having xylanase activity comprising amino acids 18 to 364 of SEQ ID NO: 2; and   (e) a polynucleotide encoding a polypeptide having xylanase activity comprising nucleotides 52 to 1145 of SEQ ID NO: 1.   
     
     
         62 . The recombinant host cell of  claim 61 , wherein the polynucleotide encodes a polypeptide having xylanase activity comprising an amino acid sequence having at least 95% identity to amino acids 18 to 364 of SEQ ID NO: 2. 
     
     
         63 . The recombinant host cell of  claim 61 , wherein the polynucleotide encodes a polypeptide having xylanase activity comprising an amino acid sequence having at least 97% identity to amino acids 18 to 364 of SEQ ID NO: 2. 
     
     
         64 . The recombinant host cell of  claim 61 , wherein the polynucleotide encodes a polypeptide having xylanase activity comprising an amino acid sequence having at least 98% identity to amino acids 18 to 364 of SEQ ID NO: 2. 
     
     
         65 . The recombinant host cell of  claim 61 , wherein the polynucleotide encodes a polypeptide having xylanase activity comprising an amino acid sequence having at least 99% identity to amino acids 18 to 364 of SEQ ID NO: 2. 
     
     
         66 . The recombinant host cell of  claim 61 , wherein the polynucleotide encodes a polypeptide having xylanase activity comprising the amino acid sequence of SEQ ID NO: 2, or a fragment thereof having xylanase activity. 
     
     
         67 . The recombinant host cell of  claim 61 , wherein the polynucleotide encodes a polypeptide having xylanase activity comprising the amino acid sequence of SEQ ID NO: 2. 
     
     
         68 . The recombinant host cell of  claim 61 , wherein the polynucleotide encodes a polypeptide having xylanase activity comprising amino acids 18 to 364 of SEQ ID NO: 2. 
     
     
         69 . A method for producing a polypeptide having xylanase activity comprising: (a) cultivating the recombinant host cell of  claim 61  under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.