US2018292391A1PendingUtilityA1
Gram-positive bacteria specific binding compounds
Est. expiryJul 15, 2029(~3 yrs left)· nominal 20-yr term from priority
Inventors:Tim BeaumontMark Jeroen KwakkenbosEric J. BrownJohn Hiroshi MorisakiWouter HazenbosSanjeev MariathasanKimberly KajiharaYi Xia
A61P 31/04A61P 37/04A61P 37/06A61P 31/00C07K 2317/52C07K 2317/56C07K 16/1271C07K 2317/21G01N 2469/10C07K 2317/565C07K 2317/567G01N 33/6854C07K 16/1278G01N 33/6893G01N 2800/26G01N 33/5091A61K 39/395A61K 47/42A61K 38/18C07K 14/475C07K 16/28C07K 16/22A61K 39/00A61K 48/00C07K 16/12
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Claims
Abstract
The present invention provides improved binding compounds capable of specifically binding Gram-positive bacteria. Binding compounds are provided that are fully human, enabling therapeutic applications in human individuals.
Claims
exact text as granted — not AI-modified1 - 43 . (canceled)
44 . A method for isolating S. aureus and/or S. epidermidis bacteria comprising contacting a sample with an antibody or functional part thereof, wherein the antibody or functional part thereof comprises a heavy chain variable domain and a light chain variable domain comprising:
(a) a heavy chain CDR1 sequence RFAMS (SEQ ID NO:1), and (b) a heavy chain CDR2 sequence SINNGNNPYYARSVQY (SEQ ID NO:2) or SINSGNNPYYARSVQY (SEQ ID NO:60), and (c) a heavy chain CDR3 sequence DHPSSGWPTFDS (SEQ ID NO:3), and (d) a light chain CDR1 sequence RASENVGDWLA (SEQ ID NO:4), and (e) a light chain CDR2 sequence KTSILES (SEQ ID NO:5), and (f) a light chain CDR3 sequence QHYXRFPYT (SEQ ID NO:6), wherein X is I or M.
45 . The method of claim 44 , wherein X in SEQ ID NO:6 is I.
46 . The method of claim 44 , wherein X in SEQ ID NO:6 is M.
47 . The method of claim 44 , wherein the heavy chain variable domain amino acid sequence comprises:
(SEQ ID NO: 7)
EVQLLESGGGLVQPGGSLRLSCAASGFTLSRFAMSWVRQAPGRGLEWVAS
INNGNNPYYARSVQYRFTVSRDVSQNTVSLQMNNLRAEDSATYFCAKDHP
SSGWPTFDSWGPGTLVTVSS.
48 . The method of claim 44 , wherein the heavy chain variable domain amino acid sequence comprises:
(SEQ ID NO: 9)
EVQLVESGGGLVQPGGSLRLSCAASGFTLSRFAMSWVRQAPGRGLEWVAS
INNGNNPYYARSVQYRFTVSRDVSQNTVSLQMNNLRAEDSATYFCAKDHP
SSGWPTFDSWGPGTLVTVSS.
49 . The method of claim 44 , wherein the heavy chain variable domain amino acid sequence comprises:
(SEQ ID NO: 62)
EVQLVESGGGLVQPGGSLRLSCAASGFTLSRFAMSWVRQAPGRGLEWVAS
INSGNNPYYARSVQYRFTVSRDVSQNTVSLQMNNLRAEDSATYFCAKDHP
SSGWPTFDSWGPGTLVTVSS.
50 . The method of claim 44 , wherein the light chain variable domain amino acid sequence comprises:
(SEQ ID NO: 8)
DIQLTQSPSALPASVGDRVSITCRASENVGDWLAWYRQKPGKAPNLLIYK
TSILESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYXRFPYTFGQ
GTKLEIKRTV, wherein X is Ior M.
51 . The method of claim 50 , wherein X in SEQ ID NO:8 is I.
52 . The method of claim 44 , wherein the light chain variable domain amino acid sequence comprises:
(SEQ ID NO: 10)
DIQLTQSPSALPASVGDRVSITCRASENVGDWLAWYRQKPGKAPNLLIYK
TSILESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYXRFPYTFGQ
GTKLEIKRA, wherein X is I or M.
53 . The method of claim 52 , wherein X in SEQ ID NO:10 is M.
54 . The method of claim 44 , wherein the light chain variable domain amino acid sequence comprises:
(SEQ ID NO: 11)
DIQLTQSPSALPASVGDRVSITCRASENVGDWLAWYRQKPGKAPNLLIYK
TSILESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYXRFPYTFGQ
GTKVEIKRTV, wherein X is I or M.
55 . The method of claim 54 , wherein X in SEQ ID NO:11 is M.
56 . The method of claim 44 , wherein
(a) the heavy chain variable domain amino acid sequence comprises
(SEQ ID NO: 7)
EVQLLESGGGLVQPGGSLRLSCAASGFTLSRFAMSWVRQAPGRGLEWVAS
INNGNNPYYARSVQYRFTVSRDVSQNTVSLQMNNLRAEDSATYFCAKDHP
SSGWPTFDSWGPGTLVTVSS;
and
(b) the light chain variable domain amino acid sequence comprises
(SEQ ID NO: 11)
DIQLTQSPSALPASVGDRVSITCRASENVGDWLAWYRQKPGKAPNLLIYK
TSILESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYXRFPYTFGQ
GTKVEIKRTV, wherein X is I or M.
57 . The method of claim 44 , wherein
(a) the heavy chain variable domain amino acid sequence comprises
(SEQ ID NO: 9)
EVQLVESGGGLVQPGGSLRLSCAASGFTLSRFAMSWVRQAPGRGLEWVAS
INNGNNPYYARSVQYRFTVSRDVSQNTVSLQMNNLRAEDSATYFCAKDHP
SSGWPTFDSWGPGTLVTVSS;
and
(b) the light chain variable domain amino acid sequence comprises
(SEQ ID NO: 8)
DIQLTQSPSALPASVGDRVSITCRASENVGDWLAWYRQKPGKAPNLLIYK
TSILESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYXRFPYTFGQ
GTKLEIKRTV, wherein X is I or M.
58 . The method of claim 44 , wherein
(a) the heavy chain variable domain amino acid sequence comprises
(SEQ ID NO: 7)
EVQLLESGGGLVQPGGSLRLSCAASGFTLSRFAMSWVRQAPGRGLEWVAS
INNGNNPYYARSVQYRFTVSRDVSQNTVSLQMNNLRAEDSATYFCAKDHP
SSGWPTFDSWGPGTLVTVSS;
and
(b) the light chain variable domain amino acid sequence comprises
(SEQ ID NO: 8)
DIQLTQSPSALPASVGDRVSITCRASENVGDWLAWYRQKPGKAPNLLIYK
TSILESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYXRFPYTFGQ
GTKLEIKRTV, wherein X is I or M.
59 . The method of claim 58 , wherein X in SEQ ID NO:8 is I.
60 . The method of claim 44 , wherein
(a) the heavy chain variable domain amino acid sequence comprises
(SEQ ID NO: 7)
EVQLLESGGGLVQPGGSLRLSCAASGFTLSRFAMSWVRQAPGRGLEWVAS
INNGNNPYYARSVQYRFTVSRDVSQNTVSLQMNNLRAEDSATYFCAKDHP
SSGWPTFDSWGPGTLVTVSS;
and
(b) the light chain variable domain amino acid sequence comprises
(SEQ ID NO: 10)
DIQLTQSPSALPASVGDRVSITCRASENVGDWLAWYRQKPGKAPNLLIYK
TSILESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYXRFPYTFGQ
GTKLEIKRA, wherein X is I or M.
61 . The method of claim 44 , wherein
(a) the heavy chain variable domain amino acid sequence comprises
(SEQ ID NO: 9)
EVQLVESGGGLVQPGGSLRLSCAASGFTLSRFAMSWVRQAPGRGLEWVAS
INNGNNPYYARSVQYRFTVSRDVSQNTVSLQMNNLRAEDSATYFCAKDHP
SSGWPTFDSWGPGTLVTVSS;
and
(b) the light chain variable domain amino acid sequence comprises
(SEQ ID NO: 10)
DIQLTQSPSALPASVGDRVSITCRASENVGDWLAWYRQKPGKAPNLLIYK
TSILESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYXRFPYTFGQ
GTKLEIKRA, wherein X is I or M.
62 . The method of claim 61 , wherein X in SEQ ID NO:10 is M.
63 . The method of claim 44 , wherein
(a) the heavy chain variable domain amino acid sequence comprises
(SEQ ID NO: 9)
EVQLVESGGGLVQPGGSLRLSCAASGFTLSRFAMSWVRQAPGRGLEWVAS
INNGNNPYYARSVQYRFTVSRDVSQNTVSLQMNNLRAEDSATYFCAKDHP
SSGWPTFDSWGPGTLVTVSS;
and
(b) the light chain variable domain amino acid sequence comprises
(SEQ ID NO: 11)
DIQLTQSPSALPASVGDRVSITCRASENVGDWLAWYRQKPGKAPNLLIYK
TSILESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYXRFPYTFGQ
GTKVEIKRTV, wherein X is I or M.
64 . The method of claim 63 , wherein X in SEQ ID NO:11 is M.
65 . The method of claim 44 , wherein
(a) the heavy chain variable domain amino acid sequence comprises
(SEQ ID NO: 62)
EVQLVESGGGLVQPGGSLRLSCAASGFTLSRFAMSWVRQAPGRGLEWVAS
INSGNNPYYARSVQYRFTVSRDVSQNTVSLQMNNLRAEDSATYFCAKDHP
SSGWPTFDSWGPGTLVTVSS
and
(b) the light chain variable domain amino acid sequence comprises
(SEQ ID NO: 11)
DIQLTQSPSALPASVGDRVSITCRASENVGDWLAWYRQKPGKAPNLLIYK
TSILESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYXRFPYTFGQ
GTKVEIKRTV,
wherein X is Ior M.
66 . The method of claim 65 , wherein X in SEQ ID NO:11 is M.
67 . The method of claim 44 , wherein
(a) the heavy chain variable domain amino acid sequence comprises
(SEQ ID NO: 62)
EVQLVESGGGLVQPGGSLRLSCAASGFTLSRFAMSWVRQAPGRGLEWVAS
INSGNNPYYARSVQYRFTVSRDVSQNTVSLQMNNLRAEDSATYFCAKDHP
SSGWPTFDSWGPGTLVTVSS
and
(b) the light chain variable domain amino acid sequence comprises
(SEQ ID NO: 10)
DIQLTQSPSALPASVGDRVSITCRASENVGDWLAWYRQKPGKAPNLLIYK
TSILESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYXRFPYTFGQ
GTKLEIKRA,
wherein X is I orM.
68 . The method of claim 44 , wherein
(a) the heavy chain variable domain amino acid sequence comprises
(SEQ ID NO: 62)
EVQLVESGGGLVQPGGSLRLSCAASGFTLSRFAMSWVRQAPGRGLEWVAS
INSGNNPYYARSVQYRFTVSRDVSQNTVSLQMNNLRAEDSATYFCAKDHP
SSGWPTFDSWGPGTLVTVSS
and
(b) the light chain variable domain amino acid sequence comprises
(SEQ ID NO: 8)
DIQLTQSPSALPASVGDRVSITCRASENVGDWLAWYRQKPGKAPNLLIYK
TSILESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQHYXRFPYTFGQ
GTKLEIKRTV,
wherein X is Ior M.
69 . The method of claim 65 , wherein the antibody or functional part thereof further comprises a light chain constant domain comprising the amino acid sequence of:
(SEQ ID NO: 65)
AAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQ
ESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPCTKSFN
RGEC.
70 . The method of claim 65 , wherein the antibody or functional part thereof further comprises a heavy chain constant domain comprising the amino acid sequence of:
(SEQ ID NO: 66)
CSTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGV
HTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP
KSCDKTHTCP.
71 . The method of claim 65 , wherein the antibody or functional part thereof further comprises
(a) a heavy chain constant domain comprising the amino acid sequence of:
(SEQ ID NO: 66)
CSTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGV
HTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP
KSCDKTHTCP
and
(b) a light chain constant domain comprising the amino acid sequence of:
(SEQ ID NO: 65)
AAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQ
ESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPCTKSFN
RGEC.
72 . The method of claim 65 , wherein the antibody or functional part thereof further comprises
(a) a heavy chain constant domain comprising the sequence of:
(SEQ ID NO: 66)
CSTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGV
HTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP
KSCDKTHTCP
and
(b) a light chain constant domain comprising the sequence of:
(SEQ ID NO: 64)
AAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQ
ESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFN
RGEC.
73 . The method of claim 65 , wherein the antibody or functional part thereof further comprises
(a) a heavy chain constant domain comprising the sequence of:
(SEQ ID NO: 63)
ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGV
HTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP
KSCDKTHTCP
and;
(b) a light chain constant domain comprising the sequence of:
(SEQ ID NO: 65)
AAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQ
ESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPCTKSFN
RGEC.
74 . The method of claim 65 , wherein the antibody or functional part thereof further comprises
(a) a heavy chain constant domain comprising the sequence of:
(SEQ ID NO: 63)
ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGV
HTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP
KSCDKTHTCP
and;
(b) a light chain constant domain comprising the sequence of:
(SEQ ID NO: 64)
AAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQ
ESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFN
RGEC.
75 . The method of claim 44 , wherein the antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO:56 and a light chain comprising the amino acid sequence of SEQ ID NO:58.
76 . The method of claim 44 , wherein the antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO:55 and a light chain comprising the amino acid sequence of SEQ ID NO:57.Cited by (0)
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