Method for producing continuous cell lines

Abstract

The present invention relates to a method for production of continuous cell lines comprising providing living cells of an animal or a human, irradiating said cells with UV light, proliferating said cells and selecting multiplying cells as cells of a continuous cell line.

1 - 2 . (canceled) 
     
     
         3 . An immortalized avian non-genetically manipulated embryonic cell line, wherein the cell line is adapted to proliferating after at least 20 passages. 
     
     
         4 . The immortalized avian embryonic cell line of  claim 3 , wherein the cell line is capable of growth in serum free media. 
     
     
         5 . The immortalized avian embryonic cell line of  claim 3 , wherein the cells are grown in suspension. 
     
     
         6 . The immortalized avian embryonic cell line of  claim 3 , wherein the cells adhere to a surface during growth. 
     
     
         7 . The immortalized avian embryonic cell line of  claim 3 , wherein the cells are quail embryonic cells. 
     
     
         8 . The immortalized avian embryonic cell line of  claim 3 , wherein the cells are chicken embryonic cells. 
     
     
         9 . The immortalized avian embryonic cell line of  claim 3 , wherein the cell line includes machinery for the propagation of a virus, wherein the machinery comprises nucleic acid encoding a recombinant gene product of a virus, promoters for expression in the cell, and selection markers. 
     
     
         10 . The immortalized avian embryonic cell line of  claim 9 , wherein cell line includes the machinery for the propagation of influenza virus. 
     
     
         11 . The immortalized avian embryonic cell line of  claim 9 , wherein cell line includes the machinery for the propagation of influenza A virus. 
     
     
         12 . The immortalized avian embryonic cell line of  claim 9 , wherein the cell line includes machinery for the propagation of New Caledonia virus. 
     
     
         13 . The immortalized avian embryonic cell line of  claim 9 , wherein the cell line includes machinery for the propagation of recombinant influenza A and B viruses and vectors. 
     
     
         14 . The immortalized avian embryonic cell line of  claim 9 , where in the cell line includes machinery for the propagation of Modified Ankara Vaccinia (MVA) vector. 
     
     
         15 . The immortalized avian embryonic cell line of  claim 9 , where in the cell line includes machinery for the propagation of recombinant Modified Ankara Vaccinia vector. 
     
     
         16 . The immortalized avian embryonic cell line of  claim 9 , where in the cell line includes machinery for the propagation of a virus from a group consisting of pox viruses, coronaviruses, or flaviviruses. 
     
     
         17 . The immortalized avian embryonic cell line of  claim 9 , wherein the cell line includes a nucleic acid encoding a gene of interest.