Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

Abstract

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

1 - 17 . (canceled) 
     
     
         18 . A recombinant host cell transformed with a nucleic acid construct comprising a polynucleotide encoding a GH61 polypeptide having cellulolytic enhancing activity, wherein the polynucleotide is operably linked to one or more control sequences that direct expression of the polynucleotide in a suitable host cell, wherein the polynucleotide is heterologous to the recombinant host cell, and wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
 (a) a GH61 polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2;   (b) a GH61 polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof, wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.;   (c) a GH61 polypeptide encoded by a polynucleotide having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof;   (d) a GH61 polypeptide comprising SEQ ID NO: 2 or the mature polypeptide of SEQ ID NO: 2; and   e) a GH61 polypeptide fragment of the mature polypeptide of SEQ ID NO: 2 that has cellulolytic enhancing activity.   
     
     
         19 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2. 
     
     
         20 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide has at least 96% sequence identity to the mature polypeptide of SEQ ID NO: 2. 
     
     
         21 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2. 
     
     
         22 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide has at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 2. 
     
     
         23 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide has at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 2. 
     
     
         24 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide comprises SEQ ID NO: 2 or the mature polypeptide of SEQ ID NO: 2. 
     
     
         25 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide consists of SEQ ID NO: 2 or the mature polypeptide of SEQ ID NO: 2. 
     
     
         26 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide is encoded by a polynucleotide that hybridizes under very high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof, wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C. 
     
     
         27 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide is encoded by a polynucleotide having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof. 
     
     
         28 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide is encoded by a polynucleotide having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof. 
     
     
         29 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide is encoded by a polynucleotide having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof. 
     
     
         30 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide is encoded by a polynucleotide having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof. 
     
     
         31 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide is encoded by a polynucleotide having at least 99% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof. 
     
     
         32 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide is encoded by a polynucleotide comprising the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof. 
     
     
         33 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide is encoded by a polynucleotide consisting of the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof. 
     
     
         34 . The recombinant host cell of  claim 18 , wherein the GH61 polypeptide is fragment of the mature polypeptide of SEQ ID NO: 2 and wherein the fragment has cellulolytic enhancing activity. 
     
     
         35 . A method of producing a GH61 polypeptide having cellulolytic enhancing activity, comprising:
 (a) cultivating the recombinant host cell of  claim 18  under conditions conducive for production of the GH61 polypeptide; and optionally   (b) recovering the GH61 polypeptide.