Scavenger Compounds for Improved Sequencing-by-Synthesis

Abstract

The present invention discloses methods of applications of indole-3-propionic acid, L-carnitine and O-acetyl-L-carnitine in one or more different reactive steps of a sequencing-by-synthesis workflow. The reactive steps employing these compounds include, but are not limited to, cleaving, imaging, incorporating bases and washing. The use of these new compounds provides improved sequencing performance including, but not limited to, lower error rates, higher sequence outputs and/or longer read lengths.

1 - 37 . (canceled) 
     
     
         38 . A cleave reagent comprising i) a reducing agent, and ii a scavenger selected from the group consisting of indole-3-propionic acid and a carnitine-based compound. 
     
     
         39 . The cleave reagent of  claim 38 , wherein said reducing agent is Tris(2-carboxyethyl)phosphine). 
     
     
         40 . An extend reagent comprising polymerase, a plurality of nucleotide analogues, and a scavenger selected from the group consisting of indole-3-propionic acid and a carnitine-based compound. 
     
     
         41 . A wash reagent comprising a scavenger selected from the group consisting of indole-3-propionic acid and a carnitine-based compound, said scavenger in a buffer. 
     
     
         42 . The wash reagent of  claim 41 , wherein said buffer is a Tris buffer. 
     
     
         43 . An image reagent comprising a scavenger selected from the group consisting of indole-3-propionic acid and a carnitine-based compound, said scavenger in a buffer. 
     
     
         44 . The image reagent of  claim 43 , wherein said buffer is a Hepes buffer. 
     
     
         48 . A system comprising primers hybridized to template in solution, said solution comprising a scavenger selected from the group consisting of indole-3-propionic acid and a carnitine-based compound. 
     
     
         49 . The system of  claim 48 , wherein said hybridized primers and template are immobilized. 
     
     
         50 . The system of  claim 49 , wherein said hybridized primers and template are in a flow cell.