US2018312864A1PendingUtilityA1
Plants having increased tolerance to herbicides
Est. expiryOct 22, 2035(~9.3 yrs left)· nominal 20-yr term from priority
Inventors:Stefan TreschMihiret Tekeste SisayDoreen SchachtschabelKristin HanzlikBrigitte WestonFlorian VogtDanny GeerdinkJens Lerchl
C12N 15/8274C12N 9/1059
56
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Claims
Abstract
Provided is a plant or plant part comprising a polynucleotide encoding a wild type or mutated cellulose synthase (CESA) polypeptide, the expression of the polynucleotide confers to the plant or plant part tolerance to CESA-inhibiting herbicides, such an azines.
Claims
exact text as granted — not AI-modified1 . A plant or plant part comprising a polynucleotide encoding a mutated cellulose synthase (CESA) polypeptide, a variant thereof, the expression of said polynucleotide confers to the plant or plant part tolerance to CESA-inhibiting herbicides, wherein the variant comprises on or more of the following motifs:
i)
Motif 1a:
(SEQ ID NO: 102)
L[C/G/M/S][F/V/L/I]F[L/F][Q/H/R]YR[T/V/C/I]
[T/S/M/L][H/N]P[V/A][K/N/P/R/T/E][N/D]A[Y/F]
[P/G/A/T]LWL[T/V/I/L]SVICE[I/V]WFA[F/L/I/V/M]
SW
wherein the amino acid at position 8, and/or 23 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
ii) Motif 2a:
(SEQ ID NO: 102)
YC[I/T/V/M/S/A]LPA[F/V/I]CL[I/L/F]T[D/N/G][R/K/T/Q]
FI[I/V/T]P[E/A/Q/K][I/L][S/N/T]N[Y/F/L/I/A][A/E]
[S/G][I/M/L/A/V][W/C/F/L/V/I]F[I/M/L/V][L/S][L/S/A]
F[I/V/A/L/S/M/G[[S/C]I
wherein the amino acid at position 4, 17, and/or 24 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
iii) Motif 3a:
(SEQ ID NO: 105)
VIGG[T/V/I/A]S[A/S]H[L/F][F/L]A[V/L][F/V][Q/L]G
[L/I/M][L/F]KV[L/F/I]AG[I/V][D/N/S/E/K]T[N/S]F
[T/I]V[T/A]SK
wherein the amino acid at position 1, 3, 18, and/or 28 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
iv) Motif 4a:
(SEQ ID NO: 108)
A[V/I][N/S][S/N]G[Y/F][Q/D/E]SWGPL[F/M/L]G[K/R]L
[F/L]F[A/S][L/I/F]WV[I/V][A/V/I/L]HLYPFLKG[L/M/V]
[L/M/V(I]G
wherein the amino acid at position 9, 12, 13, 15, 19, and/or 24 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
v) Motif 5a:
(SEQ ID NO: 111)
[G/A][I/M/V][V/][A/S/V]G[V/I/F]S[Y/T/D/N]A[V/I]
[N/S][S/N]G[Y/F]
wherein the amino acid at position 8 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
vi) Motif 6a:
(SEQ ID NO: 114)
[V/I][I/V/L]VW[S/A][V/I/A]L[L/I][A/S]S[I/F][F/L]
[S/T][L/V][L/M]WV[R/K][I/V][N/D]PF
wherein the amino acid at position 9, 11, 12, and/or 14 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
vii) Motif 7a:
(SEQ ID NO: 117)
EI[L/F][L/F/M]S[R/K/N]HCP[I/L]WYGY[H/T/N/S/G][G/C]
[R/K/G]L
wherein the amino acid at position 5, 13, 15, and/or 16 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
viii) Motif 8a:
(SEQ ID NO: 120)
[F/L]KWT[A/S/T]LLI[P/T]P[T/M/L]T[V/I/L]L
wherein the amino acid at position 10 within said motif of the corresponding wildtype sequence is substituted by any other amino acid.
2 . The plant or plant part of claim 1 , wherein the polynucleotide encoding the mutated CESA polypeptide comprises the nucleic acid sequence set forth in SEQ ID NO: 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, or 98, or a homologue, variant or derivative thereof.
3 . The plant or plant part of claim 1 , wherein the mutated CESA polypeptide is a functional variant having, over the full-length of the variant, at least about 60% or more amino acid sequence identity to SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82 or 83.
4 . The plant or plant part of claim 1 , wherein the mutated CESA refers to a CESA polypeptide comprising the sequence of SEQ ID NO: 1, an orthologue, paralogue, or homologue thereof, wherein the amino acid sequence differs from the wildtype amino acid sequence at one or more positions corresponding to positions 292, 307, 872, 885, 892, 928, 930, 45, 957, 1008, 1011, 1012, 1014, 1018, 1023, 999, 1051, 1053, 1054, 1056, 832, 840, 842, 843, 982 of SEQ ID NO: 1.
5 . A seed capable of germination into a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated CESA polypeptide encoded by the polynucleotide, the expression of the mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.
6 . A plant cell of or capable of regenerating a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated CESA polypeptide encoded by the polynucleotide, the expression of the mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides, wherein the plant cell comprises the polynucleotide operably linked to a promoter.
7 . A plant cell comprising a polynucleotide operably linked to a promoter operable in a cell, the promoter capable of expressing a mutated CESA polypeptide encoded by the polynucleotide, the expression of the mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.
8 . A plant product prepared from a plant or plant part comprising in at least some of its cells cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated CESA polypeptide encoded by the polynucleotide, the expression of the mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.
9 . A progeny or descendant plant derived from a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated CESA polypeptide encoded by the polynucleotide, wherein the progeny or descendant plant comprises in at least some of its cells the polynucleotide operably linked to the promoter, the expression of the mutated CESA polypeptide conferring to the progeny or descendant plant tolerance to the CESA-inhibiting herbicides.
10 . A method for controlling weeds at a locus for growth of a plant, the method comprising: (a) applying an herbicide composition comprising CESA-inhibiting herbicides to the locus; and (b) planting a seed at the locus, wherein the seed is capable of producing a plant that comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated CESA polypeptide encoded by the polynucleotide, the expression of the mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides, wherein the mutated CESA polypeptide comprises one or more of the following motifs:
i) Motif 1a:
(SEQ ID NO: 99)
L[C/G/M/S][F/V/L/I]F[L/F][Q/H/R]YR[T/V/C/I][T/S/M/
L][H/N]P[V/A][K/N/P/R/T/E][N/D]A[Y/F][P/G/A/T]LWL
[Y/V/I/L]SVICE[I/V]WFA[F/L/I/V/M]SW[[.]]
wherein the amino acid at position 8, and/or 23 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
ii) Motif 2a:
(SEQ ID NO: 102)
YC[I/T/V/M/S/A]LPA[F/V/I]CL[I/L/F]T[D/N/G][R/K/T/
Q]FI[I/V/T]P[E/A/Q/K][I/L][S/N/T]N[Y/F/L/I/A][A/E]
[S/G][I/M/L/A/V][W/C/F/L/V/I]F[/M/L/V][L/S][L/S/A]
F[I/V/A/L/S/M/G][S/C]I
wherein the amino acid at position 4, 17, and/or 24 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
iii) Motif 3a:
(SEQ ID NO: 105)
VIGG[T/V/I/A]S[A/S]H[L/F][F/L]A[V/L][F/V][Q/L]G
[L/I/M][L/F]KV[L/F/I]AG[I/V][D/N/S/E/K]T[N/S]F
[T/I]V[T/A]SK
wherein the amino acid at position 1, 3, 18, and/or 28 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
iv) Motif 4a:
(SEQ ID NO: 108)
A[V/I][N/S][S/N]G[Y/F][Q/D/E]SWGPL[F/M/L]G[K/R]L
[F/L]F[A/S][L/I/F]WV[I/V][A/V/I/L]HLYPFLKG[L/M/V]
[L/M/V(I]G
wherein the amino acid at position 9, 12, 13, 15, 19, and/or 24 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
v) Motif 5a:
(SEQ ID NO: 111)
[G/A][I/M/V][V/][A/S/V]G[V/I/F]S[Y/T/D/N]A[V/I]
[N/S][S/N]G[Y/F]
wherein the amino acid at position 8 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
vi) Motif 6a:
(SEQ ID NO: 114)
[V/I][I/V/L]VW[S/A][V/I/A]L[L/I][A/S]S[I/F][F/L]
[S/T][L/V][L/M]WV[R/K][I/V][N/D]PF
wherein the amino acid at position 9, 11, 12, and/or 14 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
vii) Motif 7a:
(SEQ ID NO: 117)
EI[L/F][L/F/M]S[R/K/N]HCP[I/L]WYGY[H/T/N/S/G][G/C]
[R/K/G]L
wherein the amino acid at position 5, 13, 15, and/or 16 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
viii) Motif 8a:
(SEQ ID NO: 120)
[F/L]KWT[A/S/T]LLI[P/T]P[T/M/L]T[V/I/L]L
wherein the amino acid at position 10 within said motif of the corresponding wildtype sequence is substituted by any other amino acid.
11 . The method of claim 10 , wherein herbicide composition is applied to the weeds and to the plant produced by the seed.
12 . The method according to claim 10 , wherein the CESA-inhibiting herbicide comprises a compound having the Formula (I):
13 . A method of producing a plant having tolerance to CESA-inhibiting herbicide, the method comprising regenerating a plant from a plant cell transformed with transformed with a recombinant polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated CESA polypeptide encoded by the polynucleotide, the expression of the mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.
14 . A method of producing a progeny plant having tolerance to CESA-inhibiting herbicide, the method comprising: crossing a first CESA-inhibiting herbicide-tolerant plant with a second plant to produce a CESA-inhibiting herbicide-tolerant progeny plant, wherein the first plant and the progeny plant comprise in at least some of their cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated CESA polypeptide encoded by the polynucleotide, the expression of the mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.
15 . A plant or plant part comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated CESA polypeptide encoded by the polynucleotide, the expression of the mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides, wherein the plant or plant part further exhibits a second or third herbicide-tolerant trait.
16 . A method for producing a plant product from the plant of claim 1 , the method comprising processing the plant or a plant part thereof to obtain the plant product.
17 . The method of claim 16 , wherein the plant product is fodder, seed meal, oil, or seed-treatment-coated seeds.
18 . A plant product obtained from a plant or plant part thereof, wherein the plant or plant part comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated CESA polypeptide encoded by the polynucleotide, the expression of the mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides, wherein the plant or plant part further exhibits a second or third herbicide-tolerant trait.
19 . The plant product of claim 18 , wherein the product is fodder, seed meal, oil, or seed-treatment-coated seed.Cited by (0)
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