Methods and articles of manufacture for the treatment of animals

Abstract

Methods are disclosed for the collection and processing of amniotic material in animals. These methods involve collection of amniotic material directly during parturition or caesarian section in animals for the processing of regenerative wound treatments and tissue repairs without culturing or utilization of any excess manipulation of tissue.

1 .- 97 . (canceled) 
     
     
         98 . An article of manufacture comprising:
 (i) a dried particulate mixture of mechanically decellularized fetal tissue obtained from one or more animals compatible with a subject animal, the dried particulate mixture is capable of reconstitution to form a reconstituted medicament for administration to the subject animal to promote healing; and   (ii) fetal cells obtained from one or more animals that are compatible with the dried particulate mixture,   wherein combining the dried particulate mixture and the fetal cells forms a medicament comprising a particulate cellular suspension for administration to the subject animal to promote healing,   wherein the subject animal is a non-human animal.   
     
     
         99 . The article of manufacture of  claim 98 , wherein the fetal tissue and/or fetal cells comprises amnion or chorion. 
     
     
         100 . The article of manufacture of  claim 98 , wherein the dried particulate mixture is obtained from the mechanical decellularization of about 1.5-5×10 2  cm 3  of amnion/mL of reconstituted medicament. 
     
     
         101 . The article of manufacture of  claim 98 , wherein the dried particulate mixture comprises particles greater than about 100 microns in diameter. 
     
     
         102 . The article of manufacture of  claim 98 , wherein the dried particulate mixture contains particles of less than about 100 microns in diameter. 
     
     
         103 . The article of manufacture of  claim 98 , wherein the particulate cellular suspension comprises about 0.8-1.2×10 6  amnion fluid fetal cells/mL of medicament. 
     
     
         104 . The article of manufacture of  claim 98 , further comprising an air dried fetal tissue wrap obtained from one or more animals compatible with a subject animal, the fetal tissue wrap constructed and arranged for placement in juxtaposition with a site of injury to promote healing. 
     
     
         105 . The article of manufacture of  claim 98 , wherein the fetal tissue comprises amnion and/or chorion. 
     
     
         106 . The article of manufacture of  claim 104 , wherein the wrap is constructed and arranged for placing on the site of at least one of an eye injury or a corneal ulcer. 
     
     
         107 . The article of manufacture of  claim 98 , wherein the healing occurs at a site of injury that exposes a movable body element of the animal's musculoskeletal system, wherein the movable body element is a muscle, joint, ligament, or tendon, wherein the injury comprises a laceration. 
     
     
         108 . The article of manufacture of  claim 107 , wherein the healing is at a site of injury caused by a condition selected from the group consisting of chondral and fascial pathologies, tendon and ligament pathologies, surface reconstruction and articular joint path 
     
     
         109 . A method for treating an injury in a subject animal, comprising the steps of: administering the article of manufacture of  claim 98  to the periphery of an injury of a non-human animal, wherein the administering the particulate cellular suspension promotes healing of the injury,
 wherein the administering comprises any one or more of an intravenous injection, an intra-articular injection, a subcutaneous injection, a superficial injection or a nebulization. 
 
     
     
         110 . The method of  claim 109 , further comprising the step of applying an air dried fetal tissue wrap to the site of the injury. 
     
     
         111 . The method of  claim 109 , wherein the injury is debrided prior to injecting. 
     
     
         112 . The method of  claim 109 , wherein the injury is an eye injury. 
     
     
         113 . A method, comprising:
 (i) obtaining animal fetal tissue; and   (ii) decellularizing at least a portion of the animal fetal tissue to obtain a particulate mixture.   
     
     
         114 . The method of  claim 113 , further comprising reconstituting the particulate mixture through adding a biocompatible solution to the particulate mixture, wherein adding the biocompatible solution creates a reconstituted particulate mixture comprising fetal cells, wherein the reconstituted particulate mixture is suitable for treating injuries to compatible injured animals. 
     
     
         115 . The method of claim of  claim 114 , further comprising;
 (i) filtering the reconstituted particulate mixture to increase the concentration of fetal cells; and   (ii) injecting the particulate mixture into an injured animal,   wherein the injection is made to a periphery of an injury of the injured animal.   
     
     
         116 . The method of  claim 115 , wherein decellularizing at least a portion of the animal fetal tissue comprises obtaining extracellular matrix through mechanical decellularization comprising cryofractionation. 
     
     
         117 . The method of  claim 113 , further comprising obtaining animal amnion fluid.