Angiogenesis inhibitor

Abstract

An angiogenesis inhibitor effective in inhibiting angiogenesis which causes a disease is obtained by, for example, sterilely filtrating, disinfecting, and concentrating a product which is obtained as follows. That is, rice bran is extracted with hot water, the insoluble matter is filtrated, and the starch is decomposed by processing the filtrate with glucoamylase, thereby obtaining a water-soluble polysaccharide extract. Ammonium sulfate is added to a culture filtrate prepared by culturing Lentinula edodes of basidiomycetes, thereby obtaining an enzyme complex from the precipitate. This enzyme complex is added to the water-soluble polysaccharide extract, and the mixture is caused to react at a pH of 4.5 for 30 to 60 min and further react at a pH of 6.0 for 30 to 60 min, thereby biologically modifying the water-soluble polysaccharide extract and obtaining the product.

1 . An angiogenesis inhibitor containing, as an effective component, a product produced by a process comprising:
 a first process of obtaining a water-soluble polysaccharide extract by decomposing, by using glucoamylase, starch extracted from rice bran;   a second process of adding ammonium sulfate to a culture filtrate prepared by culturing  Lentinula edodes  of basidiomycetes, and obtaining an enzyme complex from a precipitate; and   a third process of biologically modifying the water-soluble polysaccharide extract obtained in the first process by adding the enzyme complex obtained in the second process to the water-soluble polysaccharide extract obtained in the first process, and causing the mixture to react at a pH of 4.0 to 5.0 for 30 to 60 min and further react at a pH of 5.5 to 6.5 for 30 to 60 min,   wherein the angiogenesis inhibitor is used to inhibit angiogenesis.   
     
     
         2 . The angiogenesis inhibitor according to  claim 1 , wherein the product is a polysaccharide complex.