METHOD FOR PREPARING RECOMBINANT PROTEINS THROUGH REDUCTION OF rnpA GENE EXPRESSION

Abstract

A method is described for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. By the disclosed method, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.

What is claimed is: 
     
         1 . A method for producing a target protein, comprising the steps of:
 producing the target protein by culturing a recombinant microorganism into which a gene encoding the target protein is introduced; and   recovering the produced target protein,   wherein a substance of reducing expression of ribonuclease P is added in the culture to increase expression of the target protein.   
     
     
         2 . The method of  claim 1 , wherein the target protein is a difficult-to-express protein or a protein having a molecular weight of 50 kDa or more. 
     
     
         3 . The method of  claim 1 , wherein the target protein is a silk protein, antibody, cytochrome, enzyme, or sortase A. 
     
     
         4 . The method of  claim 1 , wherein said substance is an sRNA. 
     
     
         5 . The method of  claim 1 , wherein said sRNA is an sRNA against an rnpA gene. 
     
     
         6 . The method of  claim 1 , wherein said sRNA has a nucleotide sequence of any one of SEQ ID NOS: 1 to 3.