Stem cells for transplantation and manufacturing method therefor

Abstract

It is intended to provide MSCs for transplantation that have an improved post-transplantation cell survival rate and engraftment rate and are highly safe with fewer adverse reactions, and a method for conveniently producing MSCs for transplantation having a high cell survival rate and engraftment rate. As means therefor, the present invention provides a stem cell for transplantation comprising an MSC capable of overexpressing IL-10.

1 .- 11 . (canceled) 
     
     
         12 . A method of engrafting an isolated mesenchymal stem cell at a transplantation site and/or a neighboring site thereof, comprising:
 transplanting the mesenchymal stem cell to a target tissue,   wherein the mesenchymal stem cell comprises an expression system overexpressing exogenous interleukin-10 (IL-10).   
     
     
         13 . The method of  claim 12 , wherein the expression system overexpresses IL-10 at least 2 times or more as compared to the IL-10 expression level in a mesenchymal stem cell without the expression system. 
     
     
         14 . The method of  claim 13 , wherein the expression system consists of an adeno associated virus (AAV) vector. 
     
     
         15 . The method of  claim 12 , wherein the expression system consists of an adeno associated virus (AAV) vector. 
     
     
         16 . The method of  claim 12 , wherein the target tissue is mesenchymal connective tissue, the central nervous system, or the liver. 
     
     
         17 . The method of  claim 13 , wherein the target tissue is mesenchymal connective tissue, the central nervous system, or the liver. 
     
     
         18 . The method of  claim 14 , wherein the target tissue is mesenchymal connective tissue, the central nervous system, or the liver.