Methods and kits for determining dna repair capacity

Abstract

In one aspect, the disclosure provides methods, compositions, vectors and kits for determining DNA repair capacity in cells or a subject.

What is claimed is: 
     
         1 . (canceled) 
     
     
         2 . A method of determining DNA repair capacity of a subject, the method comprising:
 introducing one or more DNA repair reporter vectors into cells obtained from a subject, and   determining the capacity of the cells to process the one or more DNA repair reporter vectors thereby determining the DNA repair capacity of the subject.   
     
     
         3 . The method of  claim 2 , wherein the cells obtained from the subject are blood cells. 
     
     
         4 - 30 . (canceled) 
     
     
         31 . A kit comprising one or more DNA repair reporter vectors and instructions for use of the one or more DNA repair reporter vectors. 
     
     
         32 . The kit of  claim 31 , wherein the kit comprises at least two DNA repair reporter vectors. 
     
     
         33 . The kit of  claim 31 , wherein the kit comprises at least four DNA repair reporter vectors. 
     
     
         34 . The kit of  claim 31 , wherein each DNA repair reporter vector comprises a unique DNA lesion. 
     
     
         35 . The kit of  claim 31 , wherein the DNA repair reporter vectors comprise lesions susceptible to processing by nucleotide excision repair, homologous recombination, non-homologous end joining, microhomology mediated end joining, direct reversal, base excision repair, mismatch repair or interstrand crosslink repair. 
     
     
         36 . A kit for determining the propensity of a subject to respond to a cancer treatment regimen comprising one or more DNA repair reporter vectors, wherein the one or more DNA repair reporter vectors comprise lesions representative of a cancer treatment regimen, and instructions for use of the one or more DNA repair reporter vectors. 
     
     
         37 . A kit for determining the susceptibility of a subject to an environmental condition comprising one or more DNA repair reporter vectors, wherein the one or more DNA repair reporter vectors comprise lesions representative of an environmental condition, and instructions for use of the one or more DNA repair reporter vectors. 
     
     
         38 . (canceled) 
     
     
         39 . The kit of  claim 31 , further comprising a cell line with a known DNA repair capacity. 
     
     
         40 . (canceled) 
     
     
         41 . A DNA repair reporter vector comprising a DNA lesion and a first nucleic acid sequence allowing for the identification of the DNA lesion. 
     
     
         42 . The DNA repair reporter vector of  claim 41 , further comprising a fluorescence reporter gene. 
     
     
         43 . The DNA repair reporter vector of  claim 41 , further comprising a second nucleic acid sequence allowing for the identification of the DNA repair reporter vector. 
     
     
         44 . The DNA repair reporter vector of  claim 41 , wherein the first nucleic acid sequence allows for the determination of the processing of the DNA lesion. 
     
     
         45 . The DNA repair reporter vector of  claim 44 , wherein the processing is detected by a change in the transcribed sequence of the nucleic acid sequence. 
     
     
         46 . The DNA repair reporter vector of  claim 44 , wherein the processing is detected by a change in the amount of transcribed sequence of the nucleic acid sequence. 
     
     
         47 . The DNA repair reporter vector of  claim 41 , wherein the DNA lesion is susceptible to processing by nucleotide excision repair, homologous recombination, non-homologous end joining, microhomology mediated end joining, direct reversal, base excision repair, mismatch repair or interstrand crosslink repair. 
     
     
         48 . The DNA repair reporter vector of  claim 41 , wherein the DNA lesion is representative of a cancer treatment regimen. 
     
     
         49 . The DNA repair reporter vector of  claim 41 , wherein the DNA lesion is representative of an environmental condition.