Monoclonal antibody against novel epitopes of foot-and-mouth disease virus protein 3abc and uses thereof

Abstract

This disclosure pertains to isolated antibodies or antigen binding fragments thereof that specifically bind to the 3ABC non-structural protein of Foot-and-Mouth Disease virus (FMDV), wherein the antibodies or antigen binding fragments thereof recognize the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 or SEQ ID NO: 12. Accordingly, this disclosure also pertains to polypeptides having an amino acid sequence selected from SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5 or SEQ ID NO: 12. Monoclonal antibody Mab 40C8 is also provided. The current disclosure also pertains to methods of detecting FMDV infection in an animal (including assays differentiating infected animals from vaccinated animals (DIVA)) and kits for performing the detection methods. Competitive ELISA kits comprising the antibody or antigen binding fragment thereof and immunoassay plates coated with the polypeptide comprising the amino acid sequence selected from SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and/or SEQ ID NO: 12 are also provided.

We claim: 
     
         1 . An isolated monoclonal antibody or antigen binding fragment thereof that specifically binds to 3ABC non-structural protein of Foot-and-Mouth Disease virus (FMDV), wherein the antibody or antigen binding fragment thereof specifically binds an epitope consisting essentially of SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 or SEQ ID NO: 12. 
     
     
         2 . The antibody or antigen binding fragment thereof of  claim 1  that specifically binds the amino acid sequence of SEQ ID NO: 3, SEQ ID NO: 6, or SEQ ID NO: 12 as the epitope. 
     
     
         3 . The antibody or antigen binding fragment thereof of  claim 1  that specifically binds the amino acid sequence of SEQ ID NO: 4 as the epitope. 
     
     
         4 . The antibody or antigen binding fragment thereof of  claim 1  that specifically binds the amino acid sequence of SEQ ID NO: 5 as the epitope. 
     
     
         5 . The antibody or antigen binding fragment thereof of  claim 1 , wherein the antibody is selected from a chimeric antibody, a single chain antibody, a single chain fragment variable (scFv) antibody, or a fragment antigen-binding (Fab fragment). 
     
     
         6 . The antibody or antigen binding fragment thereof of  claim 1 , wherein the antibody or antigen binding fragment thereof is conjugated to a label. 
     
     
         7 . A method of detecting FMDV infection in an animal, the method comprising contacting a sample from an animal with a monoclonal antibody according to  claim 1 . 
     
     
         8 . The method of  claim 7 , wherein the sample obtained from the animal is a body-fluid sample or a tissue sample. 
     
     
         9 . The method of  claim 8 , wherein the body-fluid sample is aqueous humor, vitreous humor, blood serum, blood plasma, cerebrospinal fluid, endolymph, perilymph, exudates, lymph, mucus, pericardial fluid, pleural fluid, synovial fluid, milk, or oral fluids. 
     
     
         10 . The method of  claim 8 , wherein the tissue sample is brain, eyes, pineal gland, pituitary gland, thyroid gland, parathyroid glands, thorax, heart, lungs, esophagus, thymus gland, pleura, adrenal glands, appendix, gall bladder, urinary bladder, large intestine, small intestine, kidneys, liver, pancreas, spleen, stoma, prostate gland, testes, ovaries, or uterus. 
     
     
         11 . A kit comprising a monoclonal antibody or antigen binding fragment according to  claim 1 , said antibody or antigen binding fragment specifically binding to 3ABC non-structural protein of FMDV, wherein the antibody or antigen binding fragment thereof specifically binds the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, or SEQ ID NO: 12. 
     
     
         12 . The kit of  claim 11 , wherein said kit further comprising an immunoassay plate coated with a polypeptide comprising the amino acid sequence selected from SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 or SEQ ID NO: 12, FMDV non-structural protein 3ABC or combinations thereof. 
     
     
         13 . The kit of  claim 11 , wherein the antibody or antigen binding fragment thereof is conjugated to a label. 
     
     
         14 . A method of producing an antibody comprising immunizing an animal with an immunogen comprising a polypeptide consisting essentially of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 or SEQ ID NO: 12, or combinations thereof; and collecting antibodies from said animal. 
     
     
         15 . A polypeptide consisting of or consisting essentially of an amino acid sequence selected from SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 or SEQ ID NO: 12. 
     
     
         16 . An immunogenic carrier protein covalently attached to a polypeptide according to  claim 15 . 
     
     
         17 . A composition of matter comprising a polypeptide according to  claim 15  bound to a substrate. 
     
     
         18 . A composition of matter comprising a substrate to which an antibody according to  claim 1  is bound.