Corn Event TC1507 And Methods For Detection Thereof

Abstract

The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize TC1507 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

1 . A plant comprising a DNA construct, said DNA construct comprising a first and a second expression cassette, wherein said first expression cassette in operable linkage comprises
 (a) a maize ubiquitin promoter;   (b) a 5′ untranslated exon of a maize ubiquitin gene;   (c) a maize ubiquitin intron;   (d) a Cry1F encoding DNA molecule; and   (e) a 3′ ORF25 transcriptional terminator; and   said second expression cassette comprising in operable linkage
 (i) a CaMV 35S promoter; 
 (ii) a pat encoding DNA molecule; and 
 (iii) a 3′ transcriptional terminator from (CaMV) 35S. 
   
     
     
         2 . (canceled) 
     
     
         3 . The plant of  claim 1 , wherein DNA having at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56 and 57 and complements thereof forms part of the plant's genome. 
     
     
         4 . The plant of  claim 1 , wherein the plant is a seed. 
     
     
         5 . (canceled) 
     
     
         6 . An insect resistant corn plant, or part thereof, wherein DNA having at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56 and 57 and complements thereof forms part of the plant's genome. 
     
     
         7 . A descent plant of the insect resistant corn plant of  claim 6  wherein DNA having at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56 and 57 and complements thereof forms part of the plant's genome. 
     
     
         8 . (canceled) 
     
     
         9 . (canceled) 
     
     
         10 . A method of producing an insect resistant corn plant comprising:
 (a) breeding with an insect resistant corn plant comprising in its genome DNA having at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56 and 57 and complements thereof; and   (b) selecting a progeny of(a) by analyzing the genome of the progeny for the presence of at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56 and 57 and complements thereof.   
     
     
         11 . The method of  claim 10 , wherein breeding comprises crossing the insect resistant corn plant of (a) with a second plant. 
     
     
         12 . The method of  claim 11 , wherein the insect resistant corn plant of (a) is the pollen parent for the crossing. 
     
     
         13 . The method of  claim 11 , wherein the second plant is the pollen parent for the crossing. 
     
     
         14 . The method of  claim 10 , wherein the progeny comprises the insect resistance of the insect resistant corn plant of (a). 
     
     
         15 . The method of  claim 10 , wherein the progeny comprises in its genome DNA having at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56 and 57 and complements thereof. 
     
     
         16 . (canceled) 
     
     
         17 . A seed of the insect resistant corn plant of  claim 15 , wherein the seed comprises in its genome DNA having at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56 and 57 and complements thereof.