Humanized anti-tau antibodies

Abstract

Provided herein is an isolated antibody or antigen-binding fragment that specifically binds tau, the antibody or fragment comprising a heavy chain variable (VH) region and a light chain variable (VL) region having amino acid sequences set forth herein. Also provided are methods of preventing or treating a tauopathy in a subject, comprising administering to a human in need of therapy for a tauopathy with one or more antibodies or fragments as described herein, wherein the antibodies or antigen-binding fragments are administered under conditions and in an amount effective to prevent or treat the tauopathy.

1 .- 59 . (canceled) 
     
     
         60 . A vector comprising a nucleic acid molecule encoding an anti-tau antibody comprising:
 a light chain comprising the amino acid sequence of SEQ ID NO:2, and   a heavy chain comprising the amino acid sequence of SEQ ID NO:5.   
     
     
         61 . The vector of  claim 60 , wherein the vector is an expression vector. 
     
     
         62 . An isolated host cell comprising the vector of  claim 61 . 
     
     
         63 . The host cell of  claim 62 , wherein the cell is a prokaryotic or eukaryotic cell. 
     
     
         64 . The host cell of  claim 63 , wherein the cell is a mammalian cell. 
     
     
         65 . A method of producing an anti-tau antibody, comprising culturing a cell of  claim 64 , thereby producing an anti-tau antibody. 
     
     
         66 . The method of  claim 65 , further comprising isolating the anti-tau antibody from culture media. 
     
     
         67 . The method of  claim 65 , wherein the expression is at least 11.3 micrograms per mL of culture media. 
     
     
         68 . The method of  claim 65 , wherein the expression is 13.1 micrograms per mL of culture media. 
     
     
         69 . The method of  claim 65 , wherein the expression is 13.4 micrograms per mL of culture media. 
     
     
         70 . The vector of  claim 60 , wherein the vector comprises a nucleic acid molecule encoding an anti-tau antibody comprising:
 the light chain comprises the amino acid sequence of SEQ ID NO: 18, and   the heavy chain comprises the amino acid sequence of SEQ ID NO: 13.   
     
     
         71 . The vector of  claim 70 , wherein the vector is an expression vector. 
     
     
         72 . An isolated host cell comprising the vector of  claim 71 . 
     
     
         73 . The host cell of  claim 72 , wherein the cell is a prokaryotic or eukaryotic cell. 
     
     
         74 . The host cell of  claim 73 , wherein the cell is a mammalian cell. 
     
     
         75 . A method of producing an anti-tau antibody, comprising culturing a cell of  claim 74 , thereby producing an anti-tau antibody. 
     
     
         76 . The method of  claim 75 , further comprising isolating the anti-tau antibody from culture media. 
     
     
         77 . The method of  claim 75 , wherein the expression is at least 11.3 micrograms per mL of culture media. 
     
     
         78 . The method of  claim 75 , wherein the expression is 13.1 micrograms per mL of culture media. 
     
     
         79 . The method of  claim 75 , wherein the expression is 13.4 micrograms per mL of culture media.