Production of dha and other lc pufas in plants

Abstract

The invention provides recombinant host organisms (e.g., plants) genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins (e.g., PPTase and/or ACoAS) that allow for and/or improve the production of PUFAs in the host organism. The present invention also relates to methods of making and using such organisms (e.g., to obtain PUFAs) as well as products obtained from such organisms (e.g., oil and/or seed).

1 - 68 . (canceled) 
     
     
         69 . A seed oil obtained from a genetically modified plant, descendant, seed, cell, tissue, or part thereof, wherein said seed oil comprises 0.01% to 15% DHA and wherein said plant is  Brassica.    
     
     
         70 . The seed oil of  claim 69 , wherein said seed oil comprises 0.01% to 10% DHA. 
     
     
         71 . The seed oil of  claim 69 , wherein said seed oil comprises 0.05% to 1% DHA. 
     
     
         72 . The seed oil of any one of  claims 69 - 71 , wherein said seed oil further comprises 0.01% to 10% EPA. 
     
     
         73 . The seed oil of any one of  claims 69 - 71 , wherein said seed oil further comprises 0.01% to 5% EPA. 
     
     
         74 . The seed oil of any one of  claims 69 - 71 , wherein said seed oil further comprises 0.05% to 1% EPA. 
     
     
         75 . A canola seed oil, wherein said seed oil is substantially free of intermediate or side products of the system for synthesizing PUFAs and that are not naturally produced by the endogenous FAS system in the wild-type plants. 
     
     
         76 . The seed oil of  claim 75 , wherein said seed oil comprises less than 7% by weight of total fatty acids of intermediate or side products of the system for synthesizing PUFAs. 
     
     
         77 . The seed oil of  claim 75 , wherein said seed oil comprises less than 5% by weight of total fatty acids of intermediate or side products of the system for synthesizing PUFAs. 
     
     
         78 . The seed oil of  claim 75 , wherein said seed oil comprises less than 3% by weight of total fatty acids of intermediate or side products of the system for synthesizing PUFAs. 
     
     
         79 . The seed oil of any one of  claims 78 - 82 , wherein said intermediate or side products of the system for synthesizing PUFAs comprises gamma-linolenic acid (GLA; 18:3, n-6), stearidonic acid (STA or SDA; 18:4, n-3), dihomo-gamma-linolenic acid (DGLA or HGLA; 20:3, n-6), arachidonic acid (ARA, C20:4, n-6), and eicosatrienoic acid (ETA; 20:3, n-9). 
     
     
         80 . The seed oil of  claim 79 , wherein said intermediate or side products of the system for synthesizing PUFAs further comprises 20:0, 20:1 (Δ5), 20:1 (Δ11), 20:2 (Δ8,11), 20:2 (Δ11,14), 20:3 (Δ5,11,14), 20:3 (Δ11,14,17), mead acid (20:3; Δ5,8,11), and 20:4 (Δ5,1,14,17). 
     
     
         81 . A seed oil obtained from a genetically modified plant, descendant, seed, cell, tissue, or part thereof, wherein said seed oil comprises 0.01% to 10% EPA and wherein said plant is  Brassica.    
     
     
         82 . The seed oil of  claim 81 , wherein said seed oil comprises 0.01% to 5% EPA. 
     
     
         83 . The seed oil of  claim 81 , wherein said seed oil comprises 0.05% to 1% EPA. 
     
     
         84 . A canola seed oil, wherein said seed oil comprises DHA and DPA, wherein the proportion of DHA is at least 70% by weight of the total amount of DHA and DPA. 
     
     
         85 . A canola seed oil, wherein said seed oil further comprises EPA, wherein the proportion of DHA is at least 70% by weight of the total amount of DHA, DPA and EPA. 
     
     
         86 . A food product for animals or humans, comprising the seed oil of any one of  claims 69  to  85 . 
     
     
         87 . The food product for animals of  claim 86 , wherein said food product is animal feed or feed additive. 
     
     
         88 . The food product for animals of  claim 87 , wherein the animal is non-ruminant, pig, poultry, or fish.