Multifunctional biological substance separation device

Abstract

The present invention provides a multifunctional biological substance separation device or dehydration device illustratively comprising a housing, a spindle push rod running through the housing, a return device, a magnetic sleeve connecting ring, and an electric actuator. An end of the spindle push rod is provided with a magnetic column. The magnetic sleeve connecting ring is provided on the spindle push rod in a sleeving manner and one end, which is positioned outside the housing, of the connecting ring is provided with a magnetic sleeve. The return device is provided in the housing and is used for enabling the spindle push rod to move in a direction away from the magnetic sleeve. The electric actuator is connected to the connecting ring and is used for enabling the connecting ring (and the magnetic sleeve provided thereon) to rotate.

1 . A multifunctional biological substance separation device comprising a housing, a spindle push rod running through the housing, a return device, a magnetic sleeve connecting ring and an electric actuator, wherein
 the two ends of the spindle push rod both protrude from the housing, and one end of the spindle push rod is provided with a magnetic column; the magnetic sleeve connecting ring is provided on the spindle push rod in a sleeving manner, and one end, which is positioned outside the housing, of the magnetic sleeve connecting ring is provided with a magnetic sleeve;   the return device is provided in the housing, and is used for enabling the spindle push rod to move in a direction away from the magnetic sleeve; the electric actuator is connected to the magnetic sleeve connecting ring, and is used for enabling the magnetic sleeve connecting ring to rotate.   
     
     
         2 . The multifunctional biological substance separation device according to  claim 1 , wherein a snap-fit part is fixedly provided in the housing; the return device comprises a first collar, a second collar and a first spring:
 the first collar, the first spring and the second collar are provided on the spindle push rod respectively in a sleeving manner, the first collar is abutted against the snap-fit part, the second collar is fixedly connected to the spindle push rod, and the two ends of the first spring are abutted against the first collar and the second collar respectively;   one end, which is positioned outside the housing, of the spindle push rod is provided with a push rod wrapping sleeve; the closed end of the push rod wrapping sleeve wraps the end portion of the spindle push rod, and the open end is abutted against the second collar.   
     
     
         3 . The multifunctional biological substance separation device according to  claim 2 , wherein a positioning sleeve is provided in the housing, and the positioning sleeve is provided at the portion, which is positioned in the housing, of the push rod wrapping sleeve; and the inner wall of the positioning sleeve is fitted with the outer wall of the push rod wrapping sleeve. 
     
     
         4 . The multifunctional biological substance separation device according to  claim 3 , further comprising a second spring and a magnetic sleeve disconnecting push rod provided on the housing, wherein one end of the second spring is fixed to the housing, the other end is abutted against one end of the magnetic sleeve disconnecting push rod, and the other end of the magnetic sleeve disconnecting push rod extends to an opening, through which the magnetic column runs, of the housing. 
     
     
         5 . The multifunctional biological substance separation device according to  claim 4 , wherein a circuit board is also provided in the housing; the circuit board is electrically connected to the electric actuator, and the circuit board is also provided with a motor adjustment knob and a charging port that both protrude from the housing; and the housing is also provided with a button switch used for controlling a power source of the circuit board. 
     
     
         6 . The multifunctional biological substance separation device according to  claim 5 , wherein a handle is provided on the outside of the housing, one end of the handle is fixed to the housing, and the other end bends towards the direction of the magnetic column. 
     
     
         7 . The multifunctional biological substance separation device according to  claim 6 , wherein:
 the electric actuator is provided in the housing, a driven gear is fixedly connected to the magnetic sleeve connecting ring, and the electric actuator is provided with a driving gear which engages with the driven gear or   the biological substance separation device further comprises a multi-head connecting bracket; the multi-head connecting bracket is connected to one end of the housing in a sleeving manner;   the spindle push rod comprises a driving rod and a plurality of driven rods; all the driven rods are arranged side by side, and are fixed by a connecting rod, and the connecting rod is fixedly connected to the driving rod; the driving rod, the driven rods and the connecting rod are all provided on the multi-head connecting bracket;   all the driven rods run through a fixing plate, and the ends, running through the fixing plate, of the driven rods are provided with magnetic columns, and the fixing plate is fixedly provided on the multi-head connecting bracket;   a magnetic sleeve connecting ring is provided on each driven rod; one end of each magnetic sleeve connecting ring is provided with a magnetic sleeve, the other end is fixedly provided with a driven gear; every two adjacent driven gears engage with each other, and the electric actuator is provided on the multi-head connecting bracket, and is used for driving the driven gears to rotate and   optionally wherein the driving rod is parallel to all the driven rods, and the connecting rod is perpendicular to the driving rod.   
     
     
         8 .- 9 . (canceled) 
     
     
         10 . The multifunctional biological substance separation device according to  claim 1 , wherein the magnetic sleeve is a cavity with an open end and a closed end, wherein the open end is provided on the magnetic sleeve connecting ring in a sleeving manner, and the closed end is provided with a plurality of protrusions. 
     
     
         11 . A dehydration device comprising: a housing, a spindle pushrod, a magnetic sleeve and an electric actuator;
 wherein the spindle pushrod extends through the housing, with both ends of the spindle protruding from the housing, and the spindle pushrod can reciprocate back and forth along the housing, and one end of the spindle pushrod is provided with a magnetic column;   the magnetic sleeve is sleeved on the magnetic column, and the magnetic column is positioned coaxially with the magnetic sleeve and located at the center of the magnetic sleeve, and one opened end of the magnetic sleeve is connected to the housing; and   the electric actuator is provided within the housing and connected with the magnetic sleeve for rotating the magnetic sleeve.   
     
     
         12 . The dehydration device according to  claim 11 , further comprising:
 a magnetic sleeve connecting ring provided on the housing, and one end of the magnetic sleeve connecting ring is sleeved with the magnetic sleeve, the other end is connected with the electric actuator and/or   a return device provided within the housing, and used for moving the spindle pushrod toward a direction away from the magnetic sleeve.   
     
     
         13 . (canceled) 
     
     
         14 . The dehydration device according to  claim 11 , wherein a circuit board is also provided within the housing;
 the electric actuator is electrically connected with the circuit board, and a motor adjustment knob and a charging port both protruding from the housing are also provided on the circuit board; and   a push button switch for controlling the power supply of the circuit board is also provided on the housing.   
     
     
         15 . The dehydration device according to  claim 14 , wherein one end of the magnetic sleeve is opened, while the other end is closed, and the cross-section area of the magnetic sleeve is tapering along a direction from the opened end to the closed end. 
     
     
         16 . The dehydration device according to  claim 15 , wherein pluralities of protrusions are provided on an outer wall of one closed end of the magnetic sleeve and optionally wherein the number of the protrusions is even, and the protrusions are symmetrically positioned in a direction along the axis of the magnetic sleeve and optionally wherein distances between each two adjacent protrusions are equal. 
     
     
         17 .- 18 . (canceled) 
     
     
         19 . A dehydration method of using the dehydration device according to  claim 11 , the method comprising:
 1) pushing the spindle pushrod to allow the magnetic column to reach the closed end of the magnetic sleeve, and then stretching the magnetic sleeve of the dehydration device into a solid-liquid mixture containing magnetic substances;   2) adsorbing the magnetic substances to the closed end of the magnetic sleeve under the action of the magnetic column, and then removing the dehydration device from the solid-liquid mixture;   3) controlling the electric actuator to allow the magnetic sleeve to rotate, and the magnetic substances are kept in adsorption state by the action of the magnetic field, while the moisture contained in the magnetic bead surface dehydrates under the action of centrifugal force and   optionally wherein in step 1), the solid-liquid mixture comprises a DNA lysis solution or elution buffer containing a magnetic bead.   
     
     
         20 . (canceled) 
     
     
         21 . A magnetic sleeve, wherein the magnetic sleeve is a cavity with one closed end and one opened end; the cavity sequentially comprises a connecting sleeve and a body sleeve which are arranged integrally from the opened end to the closed end; a circle of convex ring is arranged on an edge of the end of the connecting sleeve. 
     
     
         22 . The magnetic sleeve according to  claim 21 , wherein impellers are arranged on an outer side of the closed end of the body sleeve and optionally the number of the impellers is an even number, and the impellers are symmetrically arranged in an axis direction of the body sleeve. 
     
     
         23 . (canceled) 
     
     
         24 . The magnetic sleeve according to  claim 22 , wherein the cross sectional area of the body sleeve is gradually tapered in the direction from the connecting sleeve to the closed end and optionally wherein the impellers extend from the closed end of the body sleeve to an external side wall of the body sleeve. 
     
     
         25 . (canceled) 
     
     
         26 . The magnetic sleeve according to  claim 21 , wherein the connecting sleeve sequentially comprises a first lantern ring and a second lantern ring in the direction from the convex ring to the body sleeve; both the first lantern ring and the second lantern ring are gradually tapered in the direction from the convex ring to the body sleeve. 
     
     
         27 . The magnetic sleeve according to  claim 26 , wherein:
 multiple reinforcing ribs are arranged on a side wall of the second lantern ring or   an outer surface of the body sleeve is a roughed surface or a twilled surface or   multiple antiskid lugs are arranged on an inner side wall of the connecting sleeve.   
     
     
         28 . The magnetic sleeve according to  claim 27 , wherein when multiple reinforcing ribs are arranged on a side wall of the second lantern ring, the reinforcing ribs surround the second lantern ring, and the distances among every two adjacent reinforcing ribs are equal. 
     
     
         29 .- 30 . (canceled)