Soybean resistant to cyst nematodes

Abstract

A transgenic soybean resistant to soybean cyst nematode (SCN), or parts thereof, including an artificial DNA construct encoding a serine hydroxymethyltransferase protein (e.g., GmSHMT). Also provided are GmSHMT alleles containing mutations R130P and Y358N along with research and breeding methods and compositions including such

What is claimed is: 
     
         1 . An agronomically elite soybean plant with soybean cyst nematode (SCN) resistance, comprising a GmSHMT allele comprising mutations P130R and N358Y operably linked to a promoter functional in said soybean plant. 
     
     
         2 . The soybean plant of  claim 1 , wherein said GmSHMT allele comprises an amino acid sequence having 95% identity to the amino acid sequence of SEQ ID NO:2, wherein amino acid position 130 is an arginine residue and amino acid position 358 is a tyrosine residue. 
     
     
         3 . The soybean plant of  claim 2 , wherein said GmSHMT allele comprises the amino acid sequence of SEQ ID NO:2, wherein amino acid position 130 is an arginine residue and amino acid position 358 is a tyrosine residue. 
     
     
         4 . The soybean plant of  claim 2 , wherein said GmSHMT allele is encoded by a polynucleotide having 95% identity to the nucleotide sequence of SEQ ID NO:1, wherein the codon corresponding to amino acid position 130 in SEQ ID NO:2 codes for an arginine residue and the codon corresponding to amino acid position 358 in SEQ ID NO:2 codes for a tyrosine residue. 
     
     
         5 . The soybean plant of  claim 4 , wherein said GmSHMT allele is encoded by a polynucleotide comprising the nucleotide sequence of SEQ ID NO:1, wherein the codon corresponding to amino acid position 130 in SEQ ID NO:2 codes for an arginine residue and the codon corresponding to amino acid position 358 in SEQ ID NO:2 codes for a tyrosine residue. 
     
     
         6 . The soybean plant of  claim 1 , wherein said promoter is a heterologous promoter. 
     
     
         7 . The soybean plant of  claim 1 , wherein said promoter is an inducible promoter. 
     
     
         8 . The soybean plant of  claim 1 , wherein said promoter is a tissue-specific promoter. 
     
     
         9 . The soybean plant of  claim 1 , wherein said promoter is a nematode-inducible promoter. 
     
     
         10 . A plant part of the soybean plant of  claim 1 . 
     
     
         11 . The plant part of  claim 10 , wherein the plant part is a cell, pollen, a leaf, a flower, a stem, a seed, or an ovule.