Cell culture medium

Abstract

The invention relates to serum-free cell culture medium, wherein the medium either contains maltose as the sole carbohydrate source, or contains maltose and at least one other saccharide as carbohydrate sources. In a preferred embodiment, the additional saccharide is a monosaccharide, preferably glucose, and the medium comprises DMEM-F12. In a preferred embodiment the cells to be cultured may be CHO or HEK293 cells. Also disclosed are methods of growing and/or culturing a cell using the cell culture medium as described herein, methods of increasing protein yield using the cell culture medium as described herein, and kits thereof.

1 . A serum-free cell culture medium comprising maltose as sole carbohydrate source or a serum-free cell culture medium comprising maltose and at least one additional saccharide as carbohydrate sources. 
     
     
         2 . (canceled) 
     
     
         3 . The serum-free cell culture medium of  claim 1 , wherein the saccharide is a monosaccharide. 
     
     
         4 . (canceled) 
     
     
         5 . The serum-free cell culture medium of  claim 1 , comprising maltose and glucose as sole carbohydrate sources. 
     
     
         6 . The serum-free cell culture medium of  claim 1 , wherein the saccharide is a polysaccharide or a disaccharide. 
     
     
         7 . The serum-free cell culture medium of  claim 6 , wherein the polysaccharide is a glucan. 
     
     
         8 - 9 . (canceled) 
     
     
         10 . The serum-free cell culture medium of  claim 1 , wherein the serum-free cell culture medium is protein-free. 
     
     
         11 . The serum-free cell culture medium of  claim 1 , wherein the serum-free cell culture medium is a chemically defined medium. 
     
     
         12 . The serum-free cell culture medium of  claim 1 , wherein the serum-free cell culture medium is a protein-free chemically defined medium (PFCDM). 
     
     
         13 . The serum-free cell culture medium according to  claim 1 , wherein the cell culture medium further comprises a hydrolysate, an enzymatic digest or yeast cell extract. 
     
     
         14 . The serum-free cell culture medium according to  claim 1 , wherein the cell culture medium is further comprises an ionic surfactant or a non-ionic surfactant. 
     
     
         15 . The serum-free cell culture medium according to  claim 1 , wherein the cell culture medium further comprises an antibiotic agent. 
     
     
         16 . The serum-free cell culture medium of  claim 1 , wherein each ingredient is present in an amount which supports the cultivation of a cell in vitro. 
     
     
         17 . The serum-free cell culture medium of  claim 1 , wherein said at least one saccharide is present at a concentration of between 0.5 g/litre to 40 g/litre. 
     
     
         18 . The serum-free cell culture medium of  claim 1 , wherein maltose is present at a concentration of between 0.5 g/litre to 40 g/litre. 
     
     
         19 . The serum-free cell culture medium of  claim 1 , wherein the serum-free cell culture medium comprises F-12 basal medium (DIEM-F12), L-glutamine, a non-ionic surfactant, geneticin and maltose, or a modified version thereof. 
     
     
         20 . (canceled) 
     
     
         21 . The serum-free cell culture medium of  claim 1 , wherein the serum-free cell culture medium is in powdered or liquid form. 
     
     
         22 . The serum-free cell culture medium of  claim 1 , wherein the cell is selected from the group consisting of a vertebrate cell, an arthropod cell, an annelid cell, a molluscs cell, a sponge cell, a jellyfish cell, an insect cell, an avian cell, a mammalian cell and a fish cell. 
     
     
         23 . The serum-free cell culture medium of  claim 22 , wherein the cell is able to metabolize the sole carbohydrate source or carbohydrate sources without requiring prior adaptation to said source/sources. 
     
     
         24 .- 25 . (canceled) 
     
     
         26 . A method of growing and/or culturing a cell or a method of increasing protein yield, wherein the method comprises growing and/or culturing a cell in a serum-free cell culture medium comprising maltose as sole carbohydrate source or a serum-free cell culture medium comprising maltose and at least one additional saccharide as carbohydrate sources. 
     
     
         27 .- 30 . (canceled) 
     
     
         31 . A method of modulating glycosylation profile of a protein, wherein the method comprises culturing a cell expressing the protein in a serum-free cell culture medium to thereby produce a cell that expresses the protein with the modulated glycosylation profile, wherein the serum-free culture comprises maltose as sole carbohydrate source or a serum-free cell culture medium comprising maltose and at least one additional saccharide as carbohydrate sources. 
     
     
         32 .- 35 . (canceled)