Systems and methods for electrochemical aspartate transaminase (ast) and alanine transaminase (alt) detection and quantification

Abstract

A system for electrochemically detecting AST and ALT includes a first sampler for producing pyruvate from ALT and a second sampler for producing pyruvate from AST. The system further includes an electrochemical test strip for receiving processed samples from the first and second samplers, the processed samples containing pyruvate. The system further includes a meter for reading the electrochemical test strip and indicating an amount of AST and ALT in the sample.

What is claimed as new and desired to be protected by Letters Patent of the United States is: 
     
         1 . A system for electrochemically detecting ALT, the system comprising:
 a sampler for producing pyruvate from ALT;   an electrochemical test strip for receiving a processed sample from the sampler, the processed sample containing pyruvate; and   a meter for reading the electrochemical test strip and indicating an amount of ALT in the sample.   
     
     
         2 . The system of  claim 1 , wherein the sampler contains L-Alanine+alpha-Ketoglutarate. 
     
     
         3 . The system of  claim 2 , wherein the electrochemical test strip includes phosphate and ferricyanide. 
     
     
         4 . The system of  claim 3 , wherein the electrochemical test strip further includes pyruvate oxidase. 
     
     
         5 . The system of  claim 4 , wherein the sampler includes a heating element. 
     
     
         6 . The system of  claim 4 , wherein the electrochemical test strip includes a heating element. 
     
     
         7 . The system of  claim 5 , wherein the sampler includes a timer. 
     
     
         8 . The system of  claim 5 , wherein the meter includes a timer. 
     
     
         9 . A system for electrochemically detecting AST, the system comprising:
 a sampler for producing pyruvate from AST;   an electrochemical test strip for receiving a processed sample from the sampler, the processed sample containing pyruvate; and   a meter for reading the electrochemical test strip and indicating an amount of AST in the sample.   
     
     
         10 . The system of  claim 9 , wherein the sampler contains L-Aspartic acid+alpha-Ketoglutarate. 
     
     
         11 . The system of  claim 10 , wherein the sampler further contains oxaloacetate decarboxylase. 
     
     
         12 . The system of  claim 11 , wherein the electrochemical test strip includes phosphate and ferricyanide. 
     
     
         13 . The system of  claim 12 , wherein the electrochemical test strip further includes pyruvate oxidase. 
     
     
         14 . The system of  claim 13 , wherein the sampler includes a heating element. 
     
     
         15 . The system of  claim 13 , wherein the electrochemical test strip includes a heating element. 
     
     
         16 . The system of  claim 14 , wherein the sampler includes a timer. 
     
     
         17 . The system of  claim 14 , wherein the meter includes a timer.