System and method for detecting lysyl oxidase-like 2 protein (loxl2) and breast cancer

Abstract

A detection system for determining lysyl oxidase-like 2 protein (LOXL2) levels in a bodily sample, includes at least one reaction solution for generating H 2 O 2 upon combination with LOXL2 in the bodily sample, the reaction solution including lysine that can be oxidatively deaminated by LOXL2 to generate α-aminoadipic-δ-semialdehyde (allysine) and hydrogen peroxide (H 2 O 2 ).

1 . A detection system for determining lysyl oxidase-like 2 protein (LOXL2) levels in a bodily sample, comprising:
 at least one reaction solution for generating H 2 O 2  upon combination with LOXL2 in the bodily sample, the reaction solution including dissolved lysine powder as a substrate that can be oxidatively deaminated by LOXL2 to generate α-aminoadipic-δ-semialdehyde (allysine) and hydrogen peroxide (H 2 O 2 );   and a biosensor for determining electrochemically the level of generated H 2 O 2 , wherein the biosensor includes a working electrode, a counter electrode and a reference electrode.   
     
     
         2 . The detection system of  claim 1 , the bodily sample comprising a bodily fluid selected from the group consisting of blood, plasma, sera, and urine. 
     
     
         3 . The detection system of  claim 1 , the working electrode and counter electrode including catalyst particles for the increasing the rate of electrochemical oxidation-reduction reaction with H 2 O 2  and providing the detection of H 2 O 2  at a lower oxidation potential than without the presence of the catalyst particles. 
     
     
         4 . The detection system of  claim 3 , the catalyst particles comprising nano-particle metallic catalysts. 
     
     
         5 . The detection system of  claim 3 , the catalyst particles comprising a unary metal (M), a binary metal (M-X), a unary metal oxide (MOy), a binary metal oxide (MOy-XOy), a metal-metal oxide composite material (M-MOy) or a combination of which, wherein y is less than 3, and M and X are independently selected from a group consisting of Li, Na, Mg, Al, K, Ca, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Sr, Y, Zr, Nb, Mo, Ru, Rh, Pd, Ag, Cd, In, Sn, Ba, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Lu, Ta, W, Os, Ir, Pt, Au, and Pb. 
     
     
         6 . The detection system of  claim 3 , the catalyst particles comprising iridium oxide particles. 
     
     
         7 . The detection system of  claim 1 , further comprising a measuring device for applying voltage potentials to the working electrode and counter electrode and measuring the current flow between the working electrode and counter electrode. 
     
     
         8 . The detection system of  claim 1 , being suitable for detection of breast cancer in a subject. 
     
     
         9 . An assay for detecting breast cancer in a subject comprising:
 at least one reaction solution for generating H 2 O 2  upon combination with LOXL2 in the bodily sample, the reaction solution including dissolved lysine powder as a substrate that can be oxidatively deaminated by LOXL2 to generate α-aminoadipic-δ-semialdehyde (allysine) and hydrogen peroxide (H 2 O 2 );   and a biosensor for electrochemically determining the level of generated H 2 O 2 , wherein the biosensor includes a working electrode, a counter electrode and a reference electrode.   
     
     
         10 . The assay of  claim 9 , the bodily sample comprising a bodily fluid selected from the group consisting of blood, plasma, sera, and urine. 
     
     
         11 . The assay of  claim 10 , the working electrode and counter electrode including catalyst particles for increasing the rate of electrochemical oxidation-reduction reaction with H 2 O 2  and providing the detection of H 2 O 2  at a lower oxidation potential than without the presence of the catalyst particles. 
     
     
         12 . The assay of  claim 11 , the catalyst particles comprising nano-particle metallic catalysts. 
     
     
         13 . The assay of  claim 11 , the catalyst particles comprising a unary metal (M), a binary metal (M-X), a unary metal oxide (MOy), a binary metal oxide (MOy-XOy), a metal-metal oxide composite material (M-MOy) or a combination of which, wherein y is less than 3, and M and X are independently selected from a group consisting of Li, Na, Mg, Al, K, Ca, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Sr, Y, Zr, Nb, Mo, Ru, Rh, Pd, Ag, Cd, In, Sn, Ba, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Lu, Ta, W, Os, Ir, Pt, Au, and Pb. 
     
     
         14 . The assay of  claim 11 , the catalyst particles comprising iridium oxide particles. 
     
     
         15 . The assay of  claim 10 , further comprising a measuring device for applying voltage potentials to the working electrode and counter electrode and measuring the current flow between the working electrode and counter electrode. 
     
     
         16 .- 27 . (canceled)