Human placental collagen compositions and methods of making and using the same

Abstract

The present invention provides compositions comprising human placental telopeptide collagen, methods of preparing the compositions, methods of their use and kits comprising the compositions. The compositions, kits and methods are useful, for example, for augmenting or replacing tissue of a mammal.

1 - 21 . (canceled) 
     
     
         22 . A process for preparing an extracellular matrix composition comprising collagen and elastin from mammalian placental tissue, said process comprising the steps of:
 (a) contacting the mammalian placental tissue with a high osmotic potential solution; and   (b) contacting the mammalian placental tissue with a low osmotic potential solution.   
     
     
         23 . The process of  claim 22  wherein the low osmotic potential solution comprises water with an osmotic potential less than that of 50 mM NaCl. 
     
     
         24 . The process of  claim 22  wherein the high osmotic potential solution has an osmotic potential of a solution of at least 0.5 M NaCl. 
     
     
         25 - 48 . (canceled) 
     
     
         49 . The process of  claim 23 , wherein the low osmotic potential solution is water. 
     
     
         50 . The process of  claim 24 , wherein the high osmotic potential solution comprises at least 1.0M NaCl. 
     
     
         51 . The process of  claim 50 , wherein the high osmotic potential solution comprises at least 2M NaCl. 
     
     
         52 . The process of  claim 22 , wherein the placental tissue comprises human placental tissue. 
     
     
         53 . The process of  claim 22 , wherein the placental tissue comprises a placental amniotic membrane that has been separated from a placental chorionic membrane. 
     
     
         54 . The process of  claim 22 , wherein the placental tissue comprises a placental chorionic membrane that has been separated from a placental amniotic membrane. 
     
     
         55 . The process of  claim 22 , wherein the placental tissue comprises a whole placenta. 
     
     
         56 . The process of  claim 52 , wherein the placental tissue is prepared from a placenta that has been exsanguinated. 
     
     
         57 . The process of  claim 22 , wherein the process further comprises drying the collagen composition. 
     
     
         58 . The process of  claim 57 , wherein the drying comprises vacuum drying, freeze drying, or heat drying. 
     
     
         59 . The process of  claim 22 , wherein one or more steps of the process are carried out under sterile conditions. 
     
     
         60 . The process of  claim 22 , wherein the process further comprises contacting the collagen composition with a biomolecule. 
     
     
         61 . The process of  claim 60 , wherein the biomolecule is an antibiotic. 
     
     
         62 . The process of  claim 22 , wherein the process does not involve contacting the mammalian placental tissue with an enzyme capable of partially or completely removing telopeptides from collagen.