Phytase

Abstract

Polypeptides having phytase activity and polynucleotide sequences encoding the phytases are provided. The gene expresses the phytase at a level of at least 7 g/l to 40 g/L. The phytases have higher specific activity, retain activity at low pH, retain activity at high temperature, increased phosphorous equivalency, increased phosphorous bioavailability, and increased phosphorous hydrolysis. The phytases can be used in a variety of industries including food, feed, ethanol production, pharmaceuticals, and cleaning.

1 . A phytase having greater phosphorous equivalency, greater phosphorous bioavailability, or greater phosphorous hydrolysis as compared to a fungal,  E. coli , or a modified  E. coli  phytase. 
     
     
         2 . The phytase of  claim 1 , wherein the phytase has a phosphorous equivalency in a range from 0.11 to 0.23%. 
     
     
         3 . The phytase of  claim 1 , wherein the phytase has at least 60 to 65% more phosphorous bioavailability when compared to a fungal or  E. coli  phytase. 
     
     
         4 . The phytase of  claim 1 , wherein the phytase has at least 20 to 30% more phosphorous bioavailability when compared to a modified  E. coli  phytase. 
     
     
         5 . The phytase of  claim 1 , wherein the phytase hydrolyzes up to 90% of phosphorous from a feed comprising phytic acid. 
     
     
         6 . The phytase of  claim 1 , wherein the phytase has at least 100% activity for more than 5 minutes at low pH. 
     
     
         7 . The phytase of  claim 1 , wherein the phytase has improved activity at phytic acid concentrations from 0.2 mM to 5.1 mM of phytic acid. 
     
     
         8 . The phytase of  claim 1 , wherein the phytase has a melting temperature greater than about 90 degrees C. 
     
     
         9 . A phytase having at least 90% activity at 2.5 minutes. 
     
     
         10 . A phytase that hydrolyzes anti-nutritive phytase IP2, IP3, IP4, IP5, and IP6 from a range of about 91% to about 98%. 
     
     
         11 . The phytase of  claim 1 , wherein the phytase has at least 85% sequence identity to SEQ ID NO:3, SEQ ID NO: 6, or SEQ ID NO: 8. 
     
     
         12 . The phytase of  claim 11 , wherein the phytase has at least 85% sequence identity to SEQ ID NO:3. 
     
     
         13 . The phytase of  claim 11 , wherein the phytase has at least 85% sequence identity to SEQ ID NO: 6. 
     
     
         14 . The phytase of  claim 11 , wherein the phytase has at least 85% sequence identity to SEQ ID NO: 8. 
     
     
         15 . The phytase of  claim 9 , wherein the phytase has at least 85% sequence identity to SEQ ID NO:3, SEQ ID NO: 6, or SEQ ID NO: 8. 
     
     
         16 . The phytase of  claim 1 , wherein the phytase has at least 90% sequence identity to SEQ ID NO:3, SEQ ID NO: 6, or SEQ ID NO: 8. 
     
     
         17 . The phytase of  claim 1 , wherein the phytase has at least 95% sequence identity to SEQ ID NO:3, SEQ ID NO: 6, or SEQ ID NO: 8. 
     
     
         18 . The phytase of  claim 17 , wherein the phytase has at least 95% sequence identity to SEQ ID NO:3. 
     
     
         19 . The phytase of  claim 17 , wherein the phytase has at least 95% sequence identity to SEQ ID NO: 6. 
     
     
         20 . The phytase of  claim 17 , wherein the phytase has at least 95% sequence identity to SEQ ID NO: 8.