Tailored oils produced from recombinant oleaginous microorganisms

Abstract

Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

1 .- 114 . (canceled) 
     
     
         115 . A method of producing a triglyceride oil, the method comprising:
 a. cultivating a recombinant oleaginous microbe, the microbe comprising an exogenous gene that encode a β-ketoacyl synthase II (KASII) peptide;   b. recovering the triglyceride oil from said recombinant oleaginous microbe; and   wherein the triglyceride oil is enriched in C18 fatty acids or reduced in C16 fatty acids.   
     
     
         116 . The method of  claim 115 , wherein the triglyceride oil is enriched in C18:0 and C18:1 fatty acids. 
     
     
         117 . The method of  claim 115 , wherein the triglyceride is reduced in C16:0 fatty acids. 
     
     
         118 . The method of  claim 115 , wherein the triglyceride oil comprises at least 60% C18:1. 
     
     
         119 . The method of  claim 115 , wherein the recombinant oleaginous microbe is microalgae. 
     
     
         120 . The method of  claim 119 , wherein the microalgae is the genus  Prototheca  or  Chlorella.    
     
     
         121 . The method of  claim 120 , wherein the microalgae is  Prototheca moriformis.    
     
     
         122 . The method of  claim 119 , wherein the exogenous nucleic acids are codon-optimized for expression in microalgae. 
     
     
         123 . The method of  claim 122 , wherein the codon optimized nucleic acids contains the most or second most preferred codon of Table 2 for at least 60% of the codons of the coding sequence. 
     
     
         124 . The method of  claim 115 , wherein the recombinant oleaginous microbe comprises a second exogenous gene, wherein the second exogenous encodes a sucrose invertase. 
     
     
         125 . A triglyceride oil produced by the method of  claim 115 . 
     
     
         126 . A recombinant oleaginous microbial cell, the recombinant microbial cell comprising an exogenous gene that encodes a β-ketoacyl synthase II (KASII) peptide, wherein fatty acids of triglyceride oil produced by the recombinant microbial cell is enriched in C18 fatty acids or reduced in C16 fatty acids. 
     
     
         127 . The recombinant oleaginous microbial cell of  claim 126 , wherein the triglyceride oil is enriched in C18:0 and C18:1 fatty acids. 
     
     
         128 . The recombinant oleaginous microbial cell of  claim 127 , wherein the triglyceride oil comprises at least 60% C18:1. 
     
     
         129 . The recombinant oleaginous microbial cell of  claim 126 , wherein the recombinant oleaginous microbe is microalgae. 
     
     
         130 . The recombinant oleaginous microbial cell of  claim 129 , wherein the microalgae is the genus  Prototheca  or  Chlorella.    
     
     
         131 . The recombinant oleaginous microbial cell of  claim 130 , wherein the microalgae is  Prototheca moriformis.