Methods for accurately measuring enzyme activity

Abstract

Provided are biological methods for measuring enzyme activity. The methods include generating reactions, and contacting each reaction generated with a set of internal standards, where each of the internal standards includes a different amount of product. The methods also include generating a standard curve for each of the reactions from the internal standards in each of the reactions.

What is claimed is: 
     
         1 . A method for accurate measurement utilizing specialized equipment, comprising the steps of:
 (a) establish a foundation for comparison in form of measured internal standards for each individual experimental segment, through accurate measurement of each standard segment, strata consisting of a group of segments, and individual samples within each segment;   (b) separate measurements of the number of samples and the attained score, for each standard segment, each test segment, and each strata including a specific group of segments;   (c) separate calculation of the mean and the confidence interval, with modification based on the specific sample size for each segment based on the data for each segment and each strata, for both the standard and test circumstances;   (d) calculation of the mathematical curve that describes the test results, based on a least squares fit, correlation coefficient, or other accepted criteria;   (e) accurate measurement of the result of the change for each sample; and   (f) calculation of the statistical significance of the test results, to establish standards that prevent distortion from the effect of factors or circumstances that are uncontrolled.