Topical Skin Care Compositions and Methods

Abstract

Topical compositions containing lysate of human parthenogenetic stem cells (hpSCs), preferably within a liposomal dispersion, that reduce the visible signs of skin aging and/or cellulite.

1 - 18 . (canceled) 
     
     
         19 . A topical composition comprising a lysate of human parthenogenetic stem cells (hpSCs) and at least one antioxidant. 
     
     
         20 . The topical composition of  claim 19  wherein the at least one antioxidant is selected from the group of Coenzyme Q10 and its analogs, ascorbic acid and its salts, ascorbyl esters of fatty acids, tocopherol and its esters, butylated hydroxybenzoic acids and their salts, and retinoids. 
     
     
         21 . The topical composition of  claim 19  wherein the lysate of hpSCs is present in the composition within a liposomal dispersion 
     
     
         22 . The topical composition of  claim 19  wherein the lysate of hpSCs is present within a liposomal dispersion at a concentration of from 1×103-1×109 hpSCs per one gram of liposomal dispersion. 
     
     
         23 . The topical composition of  claim 22  wherein the liposomal dispersion further comprises at least one lipid-protective agent. 
     
     
         24 . The topical composition of  claim 23  wherein the lipid-protective agent is a fat-soluble antioxidant selected from the group consisting of tocopheryl acetate, retinyl palmitate, butylated hydroxytoluene, butylated hydroxyanisole, ascorbyl palmitate and mixtures thereof. 
     
     
         25 . The topical composition of  claim 19  further comprising one or more of a second antioxidant, a vitamin or vitamin derivative, or a short-chain, elastin/collagen stimulating peptide. 
     
     
         26 . The topical composition of  claim 19  wherein the lysate of hpSCs are cultured in feeder-free medium. 
     
     
         27 . The topical composition of  claim 26  wherein the lysate of hpSCs are harvested and lysed without the use of proteinase inhibitors. 
     
     
         28 . The topical composition of  claim 19  wherein the lysate of hpSCs is produced by a process comprising the steps of (a) passaging and then incubating hpSCs from blastocyst inner cell mass in cell culture medium; (b) separating the hpSCs from the cell culture medium and washing the hpSCs with calcium- and magnesium-free phosphate based saline; (c) treating the hpSCs with collagenase type IV; (d) separating the collagenase from hpSCs; (e) washing the hpSCs with calcium- and magnesium-free phosphate based saline and (f) harvesting and lysing the hpSCs. 
     
     
         29 . The topical composition of  claim 21  further comprising a liposomal emulsion, the liposomal emulsion comprising Coenzyme Q10 and vitamin E or a derivative thereof. 
     
     
         30 . The topical composition of  claim 29  wherein the liposomal dispersion containing the lysate of hpSCs is present in the topical composition at 1% to 20% by weight of the composition, and the liposomal emulsion containing a combination of Coenzyme Q10 and vitamin E or a derivative thereof is present in the topical composition at 2% to 6% by weight of the composition. 
     
     
         31 . The topical composition of  claim 19  wherein the composition further comprises a vitamin B complex or vitamin P.