Insecticidal proteins and methods for their use

Abstract

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

1 . An isolated IPD073 polypeptide having greater than 80% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2. 
     
     
         2 . The isolated IPD073 polypeptide of  claim 1 , wherein the IPD073 polypeptide has insecticidal activity against a  Diabrotica  species. 
     
     
         3 . The isolated IPD073 polypeptide of  claim 2 , wherein the  Diabrotica  species is selected from  Diabrotica virgifera, Diabrotica undecimpunctata howardi, Diabrotica barberi , and  Diabrotica speciosa.    
     
     
         4 . A recombinant polynucleotide encoding the IPD073 polypeptide of  claim 1 . 
     
     
         5 . The recombinant polynucleotide of  claim 4 , wherein the polynucleotide is a non-genomic polynucleotide. 
     
     
         6 . The recombinant polynucleotide of  claim 5 , wherein the polynucleotide has codons optimized for expression in an agriculturally important crop. 
     
     
         7 . A host cell comprising the polynucleotide of  claim 4 . 
     
     
         8 . A DNA construct comprising the polynucleotide of  claim 4  operably linked to a heterologous regulatory element. 
     
     
         9 . A transgenic plant or plant cell comprising the DNA construct of  claim 8 . 
     
     
         10 . A composition comprising the IPD073 polypeptide of  claim 1 . 
     
     
         11 . A fusion protein comprising the IPD073 polypeptide of  claim 1 . 
     
     
         12 . A method for controlling an insect pest population, comprising contacting the insect pest population with the IPD073 polypeptide of  claim 1 . 
     
     
         13 . A method of inhibiting growth or killing an insect pest, comprising contacting the insect pest with a composition comprising the IPD073 polypeptide of  claim 1 . 
     
     
         14 . A method of controlling a Coleoptera insect infestation in a transgenic plant or crop comprising expressing in the plant a polynucleotide encoding the IPD073 polypeptide of  claim 1 . 
     
     
         15 . A method for controlling an insect pest population, comprising contacting the insect pest population with the transgenic plant or plant cell of  claim 9 . 
     
     
         16 . A method of inhibiting growth or killing an insect pest, comprising contacting the insect pest with the transgenic plant or plant cell of  claim 9 . 
     
     
         17 . The method of  claim 14 , wherein the insect or insect population is resistant to at least one Bt toxin. 
     
     
         18 . (canceled) 
     
     
         19 . A DNA construct comprising a polynucleotide selected from:
 a) the polynucleotide of SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 11 or SEQ ID NO: 13;   b) a polynucleotide encoding the polypeptide of SEQ ID NO: 6, SEQ ID NO: 10, SEQ ID NO: 12 or SEQ ID NO: 14; and   c) a polynucleotide encoding a polypeptide having at least 95% identity to the amino acid sequence of SEQ ID NO: 6, SEQ ID NO: 10, SEQ ID NO: 12 or SEQ ID NO: 14, wherein the polypeptide has insecticidal activity against  Diabrotica virgifera , and the polynucleotide is operably linked to a heterologous regulatory element.   
     
     
         20 . A transgenic plant or plant cell comprising the DNA construct of  claim 19 . 
     
     
         21 . (canceled) 
     
     
         22 . (canceled) 
     
     
         23 . (canceled) 
     
     
         24 . (canceled) 
     
     
         25 . A method of controlling a Coleoptera insect infestation in a transgenic plant or crop comprising expressing in the plant a polynucleotide encoding a polypeptide having at least 95% identity to the amino acid sequence of SEQ ID NO: 6, SEQ ID NO: 10, SEQ ID NO: 12 or SEQ ID NO: 14. 
     
     
         26 . (canceled) 
     
     
         27 . (canceled) 
     
     
         28 . The method of  claim 25 , wherein the insect or insect population is resistant to at least one Bt toxin. 
     
     
         29 . (canceled)