Dressings comprising platelet lysate

Abstract

A dressing comprises a delivery vehicle and platelet lysate. The dressing can also include an antimicrobial agent, a hemostatic agent, and a binder. It is contemplated that the dressing can be used for expediting hemostasis, improving antimicrobial activity, minimizing fluid loss, and accelerating wound healing when applied to a wound. The dressing would be useful in applications including military in-theater medical care and for conditions such as diabetic foot ulcers, as well as other applications.

1 . A dressing, comprising:
 a delivery vehicle; and   platelet lysate.   
     
     
         2 . The dressing of  claim 1 , wherein the delivery vehicle is woven or non-woven gauze, allograft tissue, autograft tissue, or xenograft tissue. 
     
     
         3 . The dressing of  claim 1 , wherein the dressing further comprises an antimicrobial agent. 
     
     
         4 . The dressing of  claim 3 , wherein the antimicrobial agent contains silver; or
 wherein the antimicrobial agent is silver sulfadiazine, silver nitrate, or silver chloride; or   wherein the antimicrobial agent is iodine, chlorhexidine, bacitracin, ciprofloxacin, gentamycin, or honey.   
     
     
         5 . The dressing of  claim 1 , wherein the dressing further comprises a hemostatic agent, wherein the hemostatic agent is kaolin, chitosan, a zeolite, or a styptic such as anhydrous aluminum sulfate, potassium permanganate, zinc oxide, or zinc sulfate. 
     
     
         6 . The dressing of  claim 1 , wherein the dressing further comprises a binder, wherein the binder is glycerol, casein, arabic gum, xanthan gum, corn starch, wheat starch, cellulose, gelatin, pectin, chitosan, dextran, or albumin. 
     
     
         7 . The dressing of  claim 1 , wherein the dressing further comprises an antimicrobial agent and a hemostatic agent; and wherein the delivery vehicle is a substrate. 
     
     
         8 . The dressing of  claim 7 , wherein the platelet lysate, the antimicrobial agent, and the hemostatic agent are dispersed in a single layer upon the substrate; or
 wherein the platelet lysate, the antimicrobial agent, and the hemostatic agent each form a separate layer upon the substrate.   
     
     
         9 . The dressing of  claim 1 , wherein the delivery vehicle is a hydrogel. 
     
     
         10 . The dressing of  claim 9 , wherein the hydrogel contains a hydrogel matrix comprising carboxymethylcellulose, collagen, fibrin, alginate, poly(lactic-co-glycolic acid), or polyethylene glycol. 
     
     
         11 . The dressing of  claim 10 , wherein the hydrogel matrix is swelled in phosphate buffered saline; or
 wherein the weight ratio of the platelet lysate to the hydrogel matrix is from about 0.25% to about 90%.   
     
     
         12 . The dressing of  claim 1 , wherein the delivery vehicle is a powder, and wherein the powder is carboxymethylcellulose, collagen, fibrin, alginate, poly(lactic-co-glycolic acid), or polyethylene glycol. 
     
     
         13 . The dressing of  claim 1 , wherein the delivery vehicle is a liquid carrier or a pressurized gas. 
     
     
         14 . The dressing of  claim 13 , wherein the dressing contains a surfactant and is in the form of a shampoo; or
 wherein the dressing contains a wax and is in the form of a cream; or   wherein the dressing contains a gelling agent and is in the form of a gel.   
     
     
         15 . The dressing of  claim 1 , wherein the dressing further comprises an antifungal agent. 
     
     
         16 . A method of making a bandage, comprising:
 combining a substrate with a solution that comprises platelet lysate to obtain an impregnated substrate;   freezing the impregnated substrate; and   lyophilizing the impregnated substrate to obtain the bandage.   
     
     
         17 . The method of  claim 16 , further comprising sterilizing the bandage using radiation. 
     
     
         18 . The method of  claim 16 , wherein the substrate is impregnated with a hemostatic agent prior to being combined with the solution. 
     
     
         19 . The method of  claim 16 , wherein the impregnated substrate is frozen at a temperature below −70° C. 
     
     
         20 . A method of making a dressing, comprising:
 mixing a hydrogel matrix with dry platelet lysate; and   gelling the hydrogel matrix with a liquid to obtain the dressing.   
     
     
         21 . The method of  claim 20 , wherein the hydrogel matrix comprises carboxymethylcellulose, collagen, fibrin, alginate, poly(lactic-co-glycolic acid), or polyethylene glycol; or
 wherein the weight ratio of the platelet lysate to the hydrogel matrix is from about 0.25% to about 90%; or   wherein the liquid is phosphate buffered saline; or   wherein the liquid has a pH of about 4 to about 9; or   wherein the gelling occurs at a temperature of about 25° C. to about 37° C.   
     
     
         22 . A method for treating a condition, comprising:
 applying a dressing to a body exhibiting the condition, wherein the dressing comprises a delivery vehicle and platelet lysate.   
     
     
         23 . The method of  claim 22 , wherein the dressing is in the form of a bandage, an eye drop, a spray, a shampoo, a foam, an aerosol, a gel, a cream, or a powder mixture.