Methods And Compositions For Detecting Multiple Analytes With A Single Signal

Abstract

Compositions, methods, and devices for the detection of multiple analytes with a single signal are provided.

1 - 46 . (canceled) 
     
     
         47 . A complex comprising:
 a first capture reagent affixed to a solid support;   a first analyte of interest comprising a first interaction unit and a second interaction unit;   a second analyte of interest comprising a first interaction unit and a second interaction unit;   a bridge unit;   and a signal detection unit,   wherein the first capture reagent binds to the first interaction unit of the first analyte of interest,   wherein the bridge unit comprises one or more capture reagents that independently bind to the second interaction unit of the first analyte and the first interaction unit of the second analyte of interest; and   the signal detection unit comprises a capture reagent that binds to the second interaction unit of the second analyte of interest,   wherein the first analyte of interest and the second analyte of interest are  E. coli  analytes.   
     
     
         48 . The complex of  claim 47 , wherein the first analyte of interest and the second analyte of interest are different  E. coli  analytes. 
     
     
         49 . The complex of  claim 47 , wherein the first analyte of interest and the second analyte of interest are amplicons. 
     
     
         50 . The complex of  claim 47 , wherein the first analyte of interest and the second analyte of interest are independently amplicons of Shiga 1 toxin, Shiga 2 toxin, or eae. 
     
     
         51 . The complex of  claim 47 , wherein the first analyte of interest and the second analyte of interest are independently amplicons of Shiga 1 toxin or eae. 
     
     
         52 . The complex of  claim 47 , wherein the first analyte of interest and the second analyte of interest are independently amplicons of Shiga 2 toxin or eae. 
     
     
         53 . The complex of  claim 47 , wherein the signal detection unit emits a detectable signal. 
     
     
         54 . The complex of  claim 47 , wherein the signal detection unit comprises colloidal gold, a radioactive tag, a fluorescent tag, or a chemiluminescent substrate. 
     
     
         55 . The complex of  claim 47 , wherein the signal detection unit comprises a detectable nanocrystal, a functionalized nanoparticle, an up-converting nanoparticle, a cadmium selenide/cadmium sulfide fusion nanoparticle, a quantum dot, a near-infrared fluorophore, a lanthanide cluster, a phthalocyanine, a light emitting-diode. 
     
     
         56 . A complex comprising:
 a first capture reagent affixed to a solid support;   a first analyte of interest comprising a first interaction unit and a second interaction unit;   a second analyte of interest comprising a first interaction unit and a second interaction unit;   a third analyte of interest comprising a first interaction unit and a second interaction unit;   a first bridge unit;   a second bridge unit; and   a signal detection unit comprising a second capture reagent that binds to the second interaction unit of the third analyte of interest,   wherein the first capture reagent binds to the first interaction unit of the first analyte of interest;   wherein the first bridge unit comprises one or more capture reagents that independently bind to the second interaction unit of the first analyte of interest and the first interaction unit of the second analyte of interest;   wherein the second bridge unit comprises one or more capture reagents that independently bind to the second interaction unit of the second analyte of interest and the first interaction unit of the third analyte of interest; and   wherein the first analyte of interest, the second analyte of interest, and the third analyte of interest are  E. coli  analytes.   
     
     
         57 . The complex of  claim 56 , wherein the first analyte of interest, the second analyte of interest, and the third analyte of interest are different  E. coli  analytes. 
     
     
         58 . The complex of  claim 56 , wherein the first analyte of interest, the second analyte of interest, and the third analyte of interest are amplicons. 
     
     
         59 . The complex of  claim 56 , wherein the first analyte of interest, the second analyte of interest, and the third analyte of interest are independently amplicons of Shiga 1 toxin, Shiga 2 toxin, or eae. 
     
     
         60 . The complex of  claim 56 , wherein the first analyte of interest and the second analyte of interest are independently amplicons of Shiga 1 toxin, Shiga 2 toxin, or eae 
     
     
         61 . The complex of  claim 56 , wherein the signal detection unit emits a detectable signal. 
     
     
         62 . The complex of  claim 56 , wherein the signal detection unit comprises colloidal gold, a radioactive tag, a fluorescent tag, or a chemiluminescent substrate. 
     
     
         63 . A complex comprising:
 a first analyte of interest comprising a first interaction unit and a second interaction unit;   a second analyte of interest comprising a first interaction unit and a second interaction unit;   a bridge unit;   and a signal detection unit,   wherein the bridge unit comprises one or more capture reagents that independently bind to the second interaction unit of the first analyte and the first interaction unit of the second analyte of interest; and   the signal detection unit comprises a first capture reagent that binds to the second interaction unit of the second analyte of interest,   wherein the first analyte of interest and the second analyte of interest are amplicons.   
     
     
         64 . The complex of  claim 63 , further comprising a second capture reagent affixed to a solid support; wherein the second capture reagent binds to the first interaction unit of the first analyte of interest. 
     
     
         65 . A complex comprising:
 a first analyte of interest comprising a first interaction unit and a second interaction unit;   a second analyte of interest comprising a first interaction unit and a second interaction unit;   a third analyte of interest comprising a first interaction unit and a second interaction unit;   a first bridge unit;   a second bridge unit; and   a signal detection unit comprising a first capture reagent that binds to the second interaction unit of the third analyte of interest,   wherein the first bridge unit comprises one or more capture reagents that independently bind to the second interaction unit of the first analyte of interest and the first interaction unit of the second analyte of interest;   wherein the second bridge unit comprises one or more capture reagents that independently bind to the second interaction unit of the second analyte of interest and the first interaction unit of the third analyte of interest; and   wherein the first analyte of interest, the second analyte of interest, and the third analyte of interest are amplicons.   
     
     
         66 . The complex of  claim 65 , further comprising a second capture reagent affixed to a solid support; wherein the second capture reagent binds to the first interaction unit of the first analyte of interest.