Novel glycosyl hydrolase enzymes and uses thereof

Abstract

The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.

What is claimed is: 
     
         1 . A non-naturally occurring composition comprising:
 a. a first polypeptide or a variant thereof having β-xylosidase activity, L-α-arabinofuranosidase activity, or both β-xylosidase activity and L-α-arabinofuranosidase activity; and   b. a second polypeptide or a variant thereof having xylanase activity.   
     
     
         2 . The composition of  claim 1 , wherein the first polypeptide or variant thereof is an Fv43D, Fv3A, Pf43A, Fv43E, Fv39A, Fv43A, Fv43B, Pa51A, Fo43A, Gz43A,  Trichoderma reesei  Bxl1, Fv51A, Af43A, or Pf51A polypeptide. 
     
     
         3 . The composition of  claim 1  or  2 , wherein the first polypeptide or variant thereof has β-xylosidase activity. 
     
     
         4 . The composition of  claim 3 , wherein the first polypeptide or variant thereof having β-xylosidase activity is an Fv43D, Fv3A, Pf43A, Fv43E, Fv39A, Fv43A, Fv43B, Pa51A, Fo43A, Gz43A, or  Trichoderma reesei  Bxl1 polypeptide. 
     
     
         5 . The composition of any one of  claims 1 - 4 , further comprising a third polypeptide or a variant thereof having L-α-arabinofuranosidase activity. 
     
     
         6 . The composition of  claim 5 , wherein the third polypeptide or variant thereof is an Fv51A, Af43A, Fv43B, Pa51A or Pf51A polypeptide. 
     
     
         7 . The composition of any one of  claims 1 - 4 , further comprising a third polypeptide having β-xylosidase activity. 
     
     
         8 . The composition of  claim 7 , wherein the first polypeptide is an Fv3A polypeptide or an Fv43A polypeptide, and the third polypeptide is an Fv43D, Pa51A, Gz43A,  Trichoderma reesei  Bxl1, Pf43A, Fv43E, Fv39A, Fo43A, or Fv43B polypeptide. 
     
     
         9 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fv43D polypeptide has at least 90% sequence identity to SEQ ID NO:28, or to residues (i) 20-341, (ii) 21-350, (iii) 107-341, or (iv) 107-350 of SEQ ID NO:28. 
     
     
         10 . The composition of any one of  claims 2 ,  4 ,  6  and  8 , wherein, if present, the Fv3A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2, or to residues (i) 24-766, (ii) 73-321, (iii) 73-394, (iv) 395-622, (v) 24-622, or (vi) 73-622 of SEQ ID NO:2. 
     
     
         11 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Pf43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:4, or to residues (i) 21-445, (ii) 21-301, (iii) 21-323, (iv) 21-444, (v) 302- 444; (vi) 302-445, (vii) 324-444, or (viii) 324-445 of SEQ ID NO:4. 
     
     
         12 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fv43E polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:6, or to residues (i) 19-530, (ii) 29-530, (iii) 19-300, or (iv) 29-300 of SEQ ID NO:6. 
     
     
         13 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fv39A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:8, or to residues (i) 20-439, (ii) 20-291, (iii) 145-291, or 145-439 of SEQ ID NO:8. 
     
     
         14 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fv43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:10, or to residues (i) 23-449, (ii) 23-302, (iii) 23-320, (iv) 23-448, (v) 303-448, or (vi) 321-449 of SEQ ID NO:10. 
     
     
         15 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fv43B polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:12, or to residues (i) 17-574, (ii) 27-574, (iii) 17-303, or (iv) 27-303 of SEQ ID NO:12. 
     
     
         16 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Pa51A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:14, or to residues (i) 21-676, (ii) 21- 652, (iii) 469-652, or (iv) 469-676 of SEQ ID NO:14. 
     
     
         17 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fo43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:18, or to residues (i) 21-341, (ii) 107-341, (iii) 21-348, or (iv) 107-348 of SEQ ID NO:18. 
     
     
         18 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Gz43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:16, or to residues (i) 19-340, (ii) 53-340, (iii) 19-383, or (iv) 53-383 of SEQ ID NO:16. 
     
     
         20 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the  Trichoderma reesei  Bxl1 polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:44. 
     
     
         21 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fv51A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:32, or to residues (i) 21-660, (ii) 21-645, (iii) 450-645, or (iv) 450-660 of SEQ ID NO:32. 
     
     
         22 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Af43A polypeptide has at least 90% sequence identity to SEQ ID NO:20, or to residues (i) 15-558, or (ii) 15-295 of SEQ ID NO:20. 
     
     
         23 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Pf51A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:22, or to residues (i) 21-632, (ii) 461-632, (iii) 21-642, or (iv) 461-642 of SEQ ID NO:22. 
     
     
         24 . The composition of any one of  claim 2 ,  4 ,  6 , or  8 , wherein, if present, the Fv43D polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:27, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:27, or to a fragment thereof. 
     
     
         25 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fv3A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:1, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:1, or to a fragment thereof. 
     
     
         26 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Pf43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:3, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:3, or to a fragment thereof. 
     
     
         27 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fv43E polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:5, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:5, or to a fragment thereof. 
     
     
         28 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fv39A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:7, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:7, or to a fragment thereof. 
     
     
         29 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fv43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:9, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:9, or to a fragment thereof. 
     
     
         30 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fv43B polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:11, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:11, or to a fragment thereof. 
     
     
         31 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Pa51A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:13, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:13, or to a fragment thereof. 
     
     
         32 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fo43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:17, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:17, or to a fragment thereof. 
     
     
         33 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Gz43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:15, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:15, or to a fragment thereof. 
     
     
         34 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Fv51A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:31, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:31, or to a fragment thereof. 
     
     
         35 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Af43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:19, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:19, or to a fragment thereof. 
     
     
         36 . The composition of any one of  claims 2 ,  4 ,  6 , and  8 , wherein, if present, the Pf51A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:21, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:21, or to a fragment thereof. 
     
     
         37 . The composition of any one of  claims 1 - 36 , wherein the second polypeptide is a  Trichoderma xylanase  or an  Aspergillus xylanase.    
     
     
         38 . The composition of  claim 37 , wherein the  Trichoderma xylanase  is a  Trichoderma reesei  Xyn3 polypeptide or a  Trichoderma reesei  Xyn2 polypeptide. 
     
     
         39 . The composition of  claim 38 , wherein, if present, the  Trichoderma reesei  Xyn3 polypeptide has at least 90% sequence identity to SEQ ID NO:42, or to residues 17-347 of SEQ ID NO:42. 
     
     
         40 . The composition of  claim 38 , wherein, if present, the  Trichoderma reesei  Xyn2 polypeptide has at least 90% sequence identity to SEQ ID NO:43, or to residues 33-222 of SEQ ID NO:43. 
     
     
         41 . The composition of  claim 38 , wherein, if present, the  Trichoderma reesei  Xyn3 polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:41, or by a nucleic acid that is capable of hybridizing under high stringency conditions to SEQ ID NO:41, or to a fragment thereof. 
     
     
         42 . The composition of  claim 37 , wherein the Aspergillus xylanase is an AfuXyn2 polypeptide or an AfuXyn5 polypeptide. 
     
     
         43 . The composition of  claim 42 , wherein, if present, the AfuXyn2 polypeptide has at least 90% sequence identity to SEQ ID NO:24, or to residues 19-228 of SEQ ID NO:24. 
     
     
         44 . The composition of  claim 42 , wherein, if present, the AfuXyn5 polypeptide has at least 90% sequence identity to SEQ ID NO:26, or to residues 20-313 of SEQ ID NO:26. 
     
     
         45 . The composition of  claim 42 , wherein, if present, the AfuXyn2 polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:23, or by a nucleic acid that is capable of hybridizing under high stringency to SEQ ID NO:23, or to a fragment thereof. 
     
     
         46 . The composition of  claim 42 , wherein, if present, the AfuXyn5 polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:25, or by a nucleic acid that is capable of hybridizing under stringent conditions to a complement of SEQ ID NO:25, or to a fragment thereof. 
     
     
         47 . The composition of any one of  claims 1 - 46 , further comprising one or more polypeptides having cellulase activity. 
     
     
         48 . The composition of  claim 47 , wherein the one or more polypeptides having cellulase activity are independently components of a whole cellulase. 
     
     
         49 . The composition of  claim 48 , wherein the whole cellulase is a β-glucosidase-enriched whole cellulase. 
     
     
         50 . The composition of  claim 48  or  49 , wherein the whole cellulase is a filamentous fungal whole cellulase. 
     
     
         51 . The composition of any one of  claims 48 - 50 , wherein the filamentous fungal whole cellulase is a preparation from an  Acremonium, Aspergillus, Emericella, Fusarium, Humicola, Mucor, Myceliophthora, Neurospora, Penicillium, Scytalidium, Thielavia, Chrysosporium, Phanerochaete, Tolypocladium , or  Trichoderma  species. 
     
     
         52 . The composition of any one of  claims 48 - 50 , wherein the whole cellulase is a  Chrysosporium lucknowense, Phanerochaete chrysosporium, Trichoderma reesei, Trichoderma harzianum, Trichoderma Trichoderma longibrachiatum, Trichoderma viride , or  Pencillium funiculosum  whole cellulase. 
     
     
         53 . The composition of  claim 47 , wherein the one or more polypeptides having cellulase activity are selected from a β-glucosidase, an endoglucanase, and a cellobiohydrolase. 
     
     
         54 . The composition of  claim 49  or  53 , wherein the β-glucosidase is a  Trichoderma reesei  Bgl1 polypeptide. 
     
     
         55 . The composition of  claim 54 , wherein the  Trichoderma reesei  Bgl1 polypeptide has at least 90% sequence identity to SEQ ID NO:45, or to residues 32-744 of SEQ ID NO:45. 
     
     
         56 . The composition of any one of  claims 49  and  53 - 55 , wherein the β-glucosidase polypeptide constitutes up to 50 wt. % of the total weight of cellulase enzymes in the composition. 
     
     
         57 . The composition of any one of  claims 49  and  53 - 56 , wherein the β-glucosidase polypeptide constitutes 2 wt. % to 50 wt. % of the total weight of proteins in the composition. 
     
     
         58 . The composition of any one of  claims 47 - 57 , wherein the one or more polypeptides having cellulase activity constitute 30 wt. % to 80 wt. % of the total weight of proteins in the composition. 
     
     
         59 . The composition of  claim 58 , wherein the one or more polypeptides having cellulase activity together are capable of achieving at least 0.00005 fraction product per mg protein per gram of phosphoric acid swollen cellulose (PASO) as determined by a calcofluor assay. 
     
     
         60 . The composition of any one of  claims 1 - 59 , further comprising one or more accessory proteins. 
     
     
         61 . The composition of  claim 60 , wherein the one or more accessory proteins are selected from the group consisting of mannanases, galactanases, arabinases, ligninases, amyloases, glucuronidases, proteases, esterases, lipases, xyloglucanases, glycoside hydrolase Family 61 polypeptides, CIP1, CIP2, swollenin, and expansins. 
     
     
         62 . The composition of  claim 60 , wherein the one or more accessory proteins are selected from the group consisting of CIP1-like proteins, CIP2-like proteins, cellobiose dehydrogenases, and manganese peroxidases. 
     
     
         63 . The composition of any one of  claims 60 - 62 , wherein the one or more accessory proteins constitute 1 wt. % to 50 wt. % of the total weight of proteins in the composition. 
     
     
         64 . The composition of any one of  claims 1 - 63 , capable of converting 60% or more of the hemicellulose xylan from a biomass into xylose. 
     
     
         65 . The composition of  claim 64 , capable of converting 70% or more of the hemicellulose xylan from a biomass into xylose. 
     
     
         66 . The composition of  claim 65 , capable of converting 80% or more of the hemicellulose xylan from a biomass into xylose. 
     
     
         67 . The composition of any one of  claims 64 - 66 , wherein the biomass is corncob, corn stover, corn fiber, switchgrass, sorghum, paper, pulp, or sugarcane bagasse. 
     
     
         68 . The composition of any one of  claims 64 - 66 , wherein the biomass is  Miscanthus.    
     
     
         69 . The composition of any one of  claims 1 - 68 , further comprising a biomass. 
     
     
         70 . The composition of  claim 69 , wherein the combined weight of polypeptides having xylanase activity is 1 g to 40 g per 1 kg of hemicellulose in the biomass. 
     
     
         71 . The composition of  claim 69 , wherein the combined weight of polypeptides having xylanase activity is 0.5 g to 40 g per 1 kg of hemicellulose in the biomass. 
     
     
         72 . The composition of  claim 71 , wherein the combined weight of polypetpides having xylanase activity is 0.5 g to 20 g per 1 kg of hemicellulose in the biomass. 
     
     
         73 . The composition of any one of  claims 70 - 72 , wherein the combined weight of polypeptides having xylanase activity is 2 g to 20 g per 1 kg of hemicellulose in the biomass. 
     
     
         74 . The composition of any one of  claims 69 - 73 , wherein the combined weight of polypeptides having β-xylosidase activity is 1 g to 50 g per 1 kg of hemicellulose in the biomass. 
     
     
         75 . The composition of any one of  claims 69 - 73 , wherein the combined weight of polypeptides having β-xylosidase activity is 0.5 g to 50 g per 1 kg of hemicellulose in the biomass. 
     
     
         76 . The composition of  claim 75 , wherein the combined weight of polypeptides having β-xylosidase activity is 0.5 g to 40 g per 1 kg of hemicellulose in the biomass. 
     
     
         77 . The composition of any one of  claims 74 - 76 , wherein the combined weight of polypeptides having β-xylosidase activity is 2 g to 40 g per 1 kg of hemicellulose in the biomass. 
     
     
         78 . The composition of any one of  claims 69 - 77 , wherein the combined weight of polypeptides having L-α-arabinofuranosidase activity, if present, is 0.5 g to 20 g per 1 kg of hemicellulose in the biomass. 
     
     
         79 . The composition of any one of  claims 69 - 77 , wherein the combined weight of polypetpides having L-α-arabinofuranosidase activity, if present, is 0.2 g to 20 g per 1 kg of hemicellulose in the biomass. 
     
     
         80 . The composition of any one of  claims 69 - 79 , wherein the combined weight of polypeptides having cellulase activity, if present, is 1 g to 100 g per 1 kg of cellulose in the biomass. 
     
     
         81 . A fermentation broth comprising the composition of any one of  claims 1 - 80 . 
     
     
         82 . The fermentation broth of  claim 81 , which is the fermentation broth of a filamentous fungus. 
     
     
         83 . The fermentation broth of  claim 82 , wherein the filamentous fungus is a  Trichoderma, Humicola, Fusarium, Aspergillus, Neurospora, Penicillium, Cephalosporium, Achlya, Podospora, Endothia, Mucor, Cochliobolus, Pyricularia, Phanerochaete , or  Chrysosporium.    
     
     
         84 . The fermentation broth of  claim 82 , wherein the filamentous fungus is a  Aspergillus awamori, Aspergillus fumigatus, Aspergillus foetidus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Chrysosporium lucknowense, Fusarium bactridioides, Fusarium cerealis, Fusarium crookwellense, Fusarium culmorum, Fusarium graminearum, Fusarium graminum, Fusarium heterosporum, Fusarium negundi, Fusarium oxysporum, Fusarium reticulatum, Fusarium roseum, Fusarium sambucinum, Fusarium sarcochroum, Fusarium sporotrichioides, Fusarium suiphureum, Fusarium torulosum, Fusarium trichothecioides, Fusarium venenatum, Bjerkandera adusta, Ceriporiopsis aneirina, Ceriporiopsis aneirina, Ceriporiopsis caregiea, Ceriporiopsis gilvescens, Ceriporiopsis pannocinta, Ceriporiopsis rivulosa, Ceriporiopsis subrufa, Ceriporiopsis subvermispora, Coprinus cinereus, Coriolus hirsutus, Humicola insolens, Humicola lanuginosa, Mucor miehei, Myceliophthora thermophila, Neurospora crassa, Neurospora intermedia, Penicillium purpurogenum, Penicillium canescens, Penicillium solitum, Penicillium funiculosum, Phanerochaete chrysosporium, Phiebia radiate, Pleurotus eryngii, Talaromyces flavus, Thielavia terrestris, Trametes villosa, Trametes versicolor, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma reesei , or  Trichoderma viride.    
     
     
         85 . The fermentation broth of  claim 82 , wherein the filamentous fungus is an  Aspergillus , or a  Fusarium.    
     
     
         86 . The fermentation broth of  claim 82 , wherein the filamentous fungus is a  Trichoderma reesei.    
     
     
         87 . The fermentation broth of  claim 82 , wherein the filamentous fungus is a  Penicillium funiculosum.    
     
     
         88 . The fermentation broth of any one of  claims 81 - 87 , which is a cell-free fermentation broth. 
     
     
         89 . A method of converting biomass to sugars, comprising contacting the biomass with the composition of any one of  claims 1 - 80 , or with the fermentation broth of any one of  claims 81 - 88 . 
     
     
         90 . A saccharification process comprising treating a material comprising hemicellulose with the composition of any one of  claims 1 - 80  or with the fermentation broth of any one of  claims 81 - 89 . 
     
     
         91 . The process of  claim 90 , wherein the material comprising hemicellulose is corncob, corn stover, corn fiber, switchgrass, sorghum, paper, pulp, or sugarcane bagasse. 
     
     
         92 . The process of  claim 90 , wherein the material comprising hemicellulose is  Miscanthus.    
     
     
         93 . The process of any one of  claims 90 - 92 , which yields at least 60% xylose from hemicellulose xylan of the material comprising hemicellulose. 
     
     
         94 . The process of any one of  claims 90 - 93 , further comprising recovering monosaccharides. 
     
     
         95 . An isolated, synthetic, or recombiant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:28, or to the amino acid sequence corresponding to residues (i) 20-341, (ii) 21-350, (iii) 107-341, or (iv) 107-350 of SEQ ID NO:28, which has β-xylosidase activity. 
     
     
         96 . An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:4, or to the amino acid sequence corresponding to residues (i) 21-445, (ii) 21-301, (iii) 21-323, (iv) 21-444, (v) 302-444, (vi) 302-445, (vii) 324-444, or (viii) 324-445 of SEQ ID NO:4, which has β-xylosidase activity. 
     
     
         97 . An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:6, or to the amino acid sequence corresponding to residues (i) 19-530, (ii) 29-530, (iii) 19-300, or (iv) 29-300 of SEQ ID NO:6, which has β-xylosidase activity. 
     
     
         98 . An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:8, or to the amino acid sequence corresponding to residues (i) 20-439, (ii) 20-291, (iii) 145-291, or (iv) 145-439 of SEQ ID NO:8, which has β-xylosidase activity. 
     
     
         99 . An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:10, or to the amino acid sequence corresponding to residues (i) 23-449, (ii) 23-302, (iii) 23-320, (iv) 23-448, (v) 303-448, (vi) 303-449, (vii) 321-448, or (viii) 321-449 of SEQ ID NO:10, which has β-xylosidase activity. 
     
     
         100 . An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:12, or to the amino acid sequence corresponding to residues (i) 17-574, (ii) 27-574, (iii) 17-303, or (iv) 27-303 of SEQ ID NO:12, which has β-xylosidase and/or L-α-arabinofuranosidase activities. 
     
     
         101 . An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:14, or to the amino acid sequence corresponding to residues (i) 21-676, (ii) 21-652, (iii) 469-652, or (iv) 469-676 of SEQ ID NO:14, which has β-xylosidase and/or L-α-arabinofuranosidase activities. 
     
     
         102 . An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:16, or to the amino acid sequence corresponding to residues (i) 19-340, (ii) 53-340, (iii) 19-383, or (iv) 53-383 of SEQ ID NO:16, which has β-xylosidase activity. 
     
     
         103 . An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90%% sequence identity to the amino acid sequence of SEQ ID NO:18, or to the amino acid sequence corresponding to residues (i) 21-341, (ii) 107-341, (iii) 21-348, or (iv) 107-348 of SEQ ID NO:18, which has β-xylosidase activity. 
     
     
         104 . An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:20, or to the amino acid sequence corresponding to residues (i) 15-558, or (ii) 15-295 of SEQ ID NO:20, which has L-α-arabinofuranosidase activity. 
     
     
         105 . An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:22, or to the amino acid sequence corresponding to residues (i) 21-632, (ii) 461-632, (iii) 21-642, or (iv) 461-642 of SEQ ID NO:22, which has L-α-arabinofuranosidase activity. 
     
     
         106 . An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2, or to the amino acid sequence corresponding to residues (i) 24-766, (ii) 73-321, (iii) 73-394, (iv) 395-622, (v) 24-622, or (iv) 73-622 of SEQ ID NO:2, which has β-xylosidase activity. 
     
     
         107 . An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:32, or to the amino acid sequence corresponding to residues (i) 21-660, (ii) 21-645, (iii) 450-645, or (iv) 450-660 of SEQ ID NO:32, which has L-α-arabinofuranosidase activity. 
     
     
         108 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:28, or to the amino acid sequence corresponding to residues (i) 20-341, (ii) 21-350, (iii) 107-341, or (iv) 107-350 of SEQ ID NO:28, which has β-xylosidase activity. 
     
     
         109 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:4, or to the amino acid sequence corresponding to residues (i) 21-445, (ii) 21-301, (iii) 21-323, (iv) 21-444, (v) 302-444, (vi) 302-445, (vii) 324-444, or (viii) 324-445 of SEQ ID NO:4, which has β-xylosidase activity. 
     
     
         110 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:6, or to the amino acid sequence corresponding to residues (i) 19-530, (ii) 29-530, (iii) 19-300, or (iv) 29-300 of SEQ ID NO:6, which has β-xylosidase activity. 
     
     
         111 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:8, or to the amino acid sequence corresponding to residues (i) 20-439, (ii) 20-291, (iii) 145-291, or (iv) 145-439 of SEQ ID NO:8, which has β-xylosidase activity. 
     
     
         112 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:10, or to the amino acid sequence corresponding to residues (i) 23-449, (ii) 23-302, (iii) 23-320, (iv) 23-448, (v) 303-448, (vi) 303-449, (vii) 321-448, or (viii) 321-449 of SEQ ID NO:10, which has β-xylosidase activity. 
     
     
         113 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:12, or to the amino acid sequence corresponding to residues (i) 17-574, (ii) 27-574, (iii) 17-303, or (iv) 27-303 of SEQ ID NO:12, which has β-xylosidase and/or L-α-arabinofuranosidase activities. 
     
     
         114 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:14, or to the amino acid sequence corresponding to residues (i) 21-676, (ii) 21-652, (iii) 469-652, or (iv) 469-676 of SEQ ID NO:14, which has β-xylosidase and/or L-α-arabinofuranosidase activities. 
     
     
         115 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:16, or to the amino acid sequence corresponding to residues (i) 19-340, (ii) 53-340, (iii) 19-383, or (iv) 53-383 of SEQ ID NO:16, which has β-xylosidase activity. 
     
     
         116 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:18, or to the amino acid sequence corresponding to residues (i) 21-341, (ii) 107-341, (iii) 21-348, or (iv) 107-348 of SEQ ID NO:18, which has β-xylosidase activity. 
     
     
         117 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:20, or to the amino acid sequence corresponding to residues (i) 15-558, or (ii) 15-295 of SEQ ID NO:20, which has L-α-arabinofuranosidase activity. 
     
     
         118 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:22, or to the amino acid sequence corresponding to residues (i) 21-632, (ii) 461-632, (iii) 21-642, or (iv) 461-642 of SEQ ID NO:22, which has L-α-arabinofuranosidase activity. 
     
     
         119 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2, or to the amino acid sequence corresponding to residues (i) 24-766, (ii) 73-321, (iii) 73-394, (iv) 395-622, (v) 24-622, or (iv) 73-622 of SEQ ID NO:2, which has β-xylosidase activity. 
     
     
         120 . An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:32, or to the amino acid sequence corresponding to residues (i) 21-660, (ii) 21-645, (iii) 450-645, or (iv) 450-660 of SEQ ID NO:32, which has L-α-arabinofuranosidase activity. 
     
     
         121 . A host cell engineered to recombinantly express a polypeptide of any one of  claims 108 - 120 . 
     
     
         122 . The host cell of  claim 121 , which is a cell of a filamentous fungus. 
     
     
         123 . The host cell of  claim 122 , which is a cell of a  Trichoderma, Humicola, Fusarium, Aspergillus, Neurospora, Penicillium, Cephalosporium, Achlya, Podospora, Endothia, Mucor, Cochliobolus, Pyricularia, Phanerochaete , or  Chrysosporium.    
     
     
         124 . The host cell of  claim 122 , which is a cell of a  Aspergillus awamori, Aspergillus fumigatus, Aspergillus foetidus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Chrysosporium lucknowense, Fusarium bactridioides, Fusarium cerealis, Fusarium crookwellense, Fusarium culmorum, Fusarium graminearum, Fusarium graminum, Fusarium heterosporum, Fusarium negundi, Fusarium oxysporum, Fusarium reticulatum, Fusarium roseum, Fusarium sambucinum, Fusarium sarcochroum, Fusarium sporotrichioides, Fusarium suiphureum, Fusarium torulosum, Fusarium trichothecioides, Fusarium venenatum, Bjerkandera adusta, Ceriporiopsis aneirina, Ceriporiopsis aneirina, Ceriporiopsis caregiea, Ceriporiopsis gilvescens, Ceriporiopsis pannocinta, Ceriporiopsis rivulosa, Ceriporiopsis subrufa, Ceriporiopsis subvermispora, Coprinus cinereus, Coriolus hirsutus, Humicola insolens, Humicola lanuginosa, Mucor miehei, Myceliophthora thermophila, Neurospora crassa, Neurospora intermedia, Penicillium purpurogenum, Penicillium canescens, Penicillium solitum, Penicillium funiculosum, Phanerochaete chrysosporium, Phiebia radiate, Pleurotus eryngii, Talaromyces flavus, Thielavia terrestris, Trametes villosa, Trametes versicolor, Trichoderma harzianum, Trichoderma koningii Trichoderma longibrachiatum, Trichoderma reesei , or  Trichoderma viride.    
     
     
         125 . The host cell of  claim 122  or  123 , which is a cell of a  Trichoderma spp. , a  Pencillium , an  Aspergillus , or a  Fusarium.    
     
     
         126 . The host cell of  claim 125 , wherein:
 (a) the  Trichoderma spp.  is  Trichoderma reesei ; or   (b) the  Pencillium  is  Pencillium funiculosum ; or   (c) the  Aspergillus  is  Aspergillus oryzae  or  Aspergillus nidulans ; or   (d) the  Fusarium  is  Fusarium verticillioides  or  Fusarium oxysporum.      
     
     
         127 . The host cell of  claim 121 , which is a cell of a bacterium. 
     
     
         128 . The host cell of  claim 127 , wherein the bacterium is a  Streptomyces , a  Thermomonospora , a  Bacillus , or a  Cellulomonas.    
     
     
         129 . A method of producing the composition of any one of  claims 1 - 80  or the fermentation broth of any one of  claims 81 - 88 . 
     
     
         130 . A process of saccharification comprising treating a biomass material comprising hemicellulose with the composition of any one of  claims 1 - 80  or the fermentation broth of any one of  claims 81 - 88 . 
     
     
         131 . The process of  claim 130 , wherein the biomass material further comprises cellulose. 
     
     
         132 . The process of  claim 130  or  131 , wherein the amount of polypeptides having xylanase activity is 1 g to 40 g per kg of hemicellulose in the biomass material. 
     
     
         133 . The process of  claim 130  or  131 , wherein the amount of polypeptides having xylanase activity is 0.5 g to 40 g per kg of hemicellulose in the biomass material. 
     
     
         135 . The process of any one of  claims 130 - 133 , wherein the amount of polypeptides having β-xylosidase activity is 1 g to 50 g per kg of hemicellulose in the biomass material. 
     
     
         136 . The process of any one of  claims 130 - 133 , wherein the amount of polypeptide having β-xylosidase activity is 0.5 g to 50 g per kg of hemicellulose in the biomass material. 
     
     
         137 . The process of any one of  claims 130 - 136 , wherein the amount of polypeptides having L-α-arabinofuranosidase activity is 0.5 g to 20 g per kg of hemicellulose in the biomass material. 
     
     
         138 . The process of any one of  claims 130 - 136 , wherein the amount of polypeptides having L-α-arabinofuranosidase activity is 0.2 g to 20 g per kg of hemicellulose in the biomass material. 
     
     
         139 . The process of any one of  claims 130 - 138 , wherein the amount of polypeptides having cellulase activity is 1 g to 100 g per kg of cellulose in the biomass material. 
     
     
         140 . The process of any one of  claims 130 - 139 , wherein the amount of polypeptides having β-glucosidase activity is 50% or less of the total weight of polypeptides having cellulase activity.