Compositions and method for tissue preservation

Abstract

Methods and compositions for resuscitating, storing, and preserving functional integrity of organs and tissues. Metabolic function is maintained by sustaining ATP levels, mitochondrial function, cardiomyocyte contractility, prevention of acidosis, inhibition of induction of apoptosis, maintaining ionontrophy and lusiotrophy by regulating calcium, sodium, potassium and chloride ions.

1 . A physiological salt solution comprising glutathione, ascorbic acid, arginine, citrulline, adenosine, creatine, glucose, carnitine and dichloroacetate. 
     
     
         2 . The solution of  claim 1  further comprising insulin. 
     
     
         3 . The solution of  claim 1 , wherein the glutathione is present at a concentration of 0.01-0.75 g/L. 
     
     
         4 . The solution of  claim 1 , wherein the ascorbic acid is present at a concentration of 0.01-0.5 g/L. 
     
     
         5 . The solution of  claim 1 , wherein the glucose is present at a concentration of 1.0-5.0 g/L. 
     
     
         6 . The solution of  claim 1 , wherein the carnitine is present at a concentration of 0.20-2.0 g/L. 
     
     
         7 . The solution of  claim 1 , wherein dichloroacetate is present at a concentration of 0.01-0.5 g/L. 
     
     
         8 . The solution of  claim 1 , wherein the glutathione is present at a concentration of 0.01-0.75 g/L, ascorbic acid is present at a concentration of 0.01-0.5 g/L, creatine monohydrate is present at a concentration of 0.01-0.5 g/L, glucose is present at a concentration of 1.0-5.0 g/L, carnitine is present at a concentration of 0.20-2.0 g/L, and dichloroacetate is present at a concentration of 0.01-0.5 g/L. 
     
     
         9 . The solution of  claim 1 , wherein the solution is further comprised of water and one or more of the salts selected from the group consisting of calcium chloride, potassium chloride, potassium phosphate (monobasic), magnesium chloride (hexahydrate), magnesium sulfate (heptahydrate), sodium chloride, sodium bicarbonate and sodium phosphate (dibasic; heptahydrate). 
     
     
         10 . The solution of  claim 9 , wherein the calcium chloride is present at a concentration of 0.1-0.5 g/L. 
     
     
         11 . The solution of  claim 9 , wherein the potassium chloride is present at a concentration of 0.25-0.75 g/L. 
     
     
         12 . The solution of  claim 9 , wherein the potassium phosphate (monobasic) is present at a concentration of 0.01-0.5 g/L. 
     
     
         13 . The solution of  claim 9 , wherein the magnesium chloride (hexahydrate) is present at a concentration of 0.01-0.5 g/L. 
     
     
         14 . The solution of  claim 9 , wherein the magnesium sulfate (heptahydrate) is present at a concentration of 0.01-0.5 g/L. 
     
     
         15 . The solution of  claim 9 , wherein the sodium chloride is present at a concentration of 5.0-10.0 g/L. 
     
     
         16 . The solution of  claim 9 , wherein the sodium bicarbonate is present at a concentration of 0.01-0.5 g/L. 
     
     
         17 . The solution of  claim 9 , wherein the sodium phosphate (dibasic; heptahydrate) is present at a concentration of 0.01-0.5 g/L. 
     
     
         18 . The solution of  claim 1 , wherein the solution is comprised of water, calcium chloride, potassium chloride, potassium phosphate (monobasic), magnesium chloride (hexahydrate), magnesium sulfate (heptahydrate), sodium chloride, sodium bicarbonate and sodium phosphate (dibasic; heptahydrate). 
     
     
         19 . The solution of  claim 18 , wherein the calcium chloride is present at a concentration of 0.1-0.5 g/L. 
     
     
         20 . The solution of  claim 18 , wherein the potassium chloride is present at a concentration of 0.25-0.75 g/L. 
     
     
         21 - 38 . (canceled)