Hair growth-promoting composition and method for promoting hair growth

Abstract

Provided is a hair growth-promoting composition comprising: 100 parts by weight of a stem cell culture solution obtained from a culture medium of mesenchymal stem cells which have been subcultured two or more times; 0.001 to 0.1 parts by weight of zinc; 5 to 50 parts by weight of a panthenol based compound; and 0.0001 to 0.1 parts by weight of a water-soluble vitamin, wherein it is sprayed or applied on the scalp to promote hair growth. Said hair growth-promoting composition may have an excellent effect and improve economical efficiency in treating alopecia due to a simple delivery method of the composition.

1 . A hair growth-promoting method comprising:
 obtaining 100 parts by weight of a stem cell culture solution obtained from a culture medium subjected to a process of separately adding bFGF or EGF, a growth factor, to a serum-free medium in which bovine-derived serum is not contained and culturing human adipose-derived mesenchymal stem cells which have been subcultured two or more times in the serum-free medium;   mixing 0.001 to 0.1 parts by weight of zinc, 5 to 50 parts by weight of D-panthenol, and 0.0001 to 0.1 parts by weight of D-biotin with 100 to 300 parts by weight of water to prepare a mixed solution; and   mixing the stem cell culture solution with the mixed solution and spraying or applying a mixture solution on the scalp.   
     
     
         2 . The hair growth-promoting method of  claim 1 , wherein the stem cell culture solution is a stem cell culture medium obtained by a process of (a) culturing the mesenchymal stem cells; and (b) three-dimensionally culturing mesenchymal stem cells which have been subcultured two or more times together with a biocompatible scaffold in a serum-free medium. 
     
     
         3 . The hair growth-promoting method of  claim 1 , wherein the mesenchymal stem cells are human adipose-derived stem cells.