Identification and use of target genes for control of plant parasitic nematodes

Abstract

The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.

1 - 17 . (canceled) 
     
     
         18 . A method for controlling a plant-parasitic nematode population comprising providing an agent comprising a double stranded ribonucleotide sequence that functions upon being taken up by the nematode to inhibit a biological function within said nematode, wherein the agent comprises all or a part of a nucleotide sequence selected from the group consisting of SEQ ID NO:301-SEQ ID NO:1026, SEQ ID NO:1269-SEQ ID NO:1702, and SEQ ID NO:1920-SEQ ID NO:1929 and complements thereof. 
     
     
         19 . A method for controlling a plant-parasitic nematode population comprising providing an agent comprising a first polynucleotide sequence that functions upon being taken up by the pathogen to inhibit a biological function within said nematode, wherein said polynucleotide sequence exhibits from about 95 to about 100% nucleotide sequence identity along at least from about 19 to about 25 contiguous nucleotides to a coding sequence derived from said nematode, the agent comprising a second polynucleotide sequence hybridized thereto that is complementary to said first polynucleotide sequence, and wherein said coding sequence derived from said nematode comprises a sequence selected from the group consisting of SEQ ID NO:301-SEQ ID NO:1026, SEQ ID NO:1269-SEQ ID NO:1702, and SEQ ID NO:1920-SEQ ID NO:1929, and the complements thereof. 
     
     
         20 . The method of  claim 19 , wherein said nematode is  Heterodera glycines.    
     
     
         21 - 27 . (canceled) 
     
     
         28 . A method of controlling plant nematode pest infestation in a plant comprising providing in the diet of a plant nematode pest a dsRNA comprising:
 a) a sense nucleotide sequence; and   b) an antisense nucleotide sequence complementary to said sense nucleotide sequence,   wherein said sense nucleotide sequence comprises or is complementary to a fragment of at least 21 contiguous nucleotides of a nucleic acid sequence selected from the group consisting of SEQ ID NO:301-SEQ ID NO:1026, SEQ ID NO:1269-SEQ ID NO:1702, and SEQ ID NO:1920-SEQ ID NO:1929, wherein uptake of the dsRNA by the plant nematode pest inhibits a biological function and growth of the plant nematode pest.   
     
     
         29 . The method of  claim 28 , wherein said diet comprises a plant cell transformed to express said sense and said dsRNA. 
     
     
         30 - 48 . (canceled)