Fc FUSION PROTEINS COMPRISING NOVEL LINKERS OR ARRANGEMENTS

Abstract

The application provides Fc fusion proteins having novel arrangements. In one embodiment, the application provides Fc fusion proteins comprising a 10 Fn3 domain. In another embodiment, the application provides Fc fusion proteins comprising linkers derived from the naturally occurring C-terminal tail regions of membrane bound or secretory immunoglobulins.

1 - 22 . (canceled) 
     
     
         23 . A polypeptide comprising an immunoglobulin IgG1 Fc domain and a heterologous polypeptide, wherein the heterologous polypeptide is fused to the N-terminus or the C-terminus of the Fc domain by a polypeptide linker, wherein the polypeptide linker comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 51-54, 63-65 and 84. 
     
     
         24 . The polypeptide of  claim 23 , wherein the polypeptide linker comprises SEQ ID NO: 84. 
     
     
         25 . The polypeptide of  claim 23 , wherein the heterologous polypeptide is fused to the C-terminus of the Fc domain. 
     
     
         26 . The polypeptide of  claim 23 , wherein the heterologous polypeptide is fused to the N-terminus of the Fc domain. 
     
     
         27 . The polypeptide of  claim 23 , wherein the heterologous polypeptide comprises a  10 Fn3 domain. 
     
     
         28 . The polypeptide of  claim 23 , wherein the immunoglobulin Fc domain comprises a hinge or a portion thereof. 
     
     
         29 . The polypeptide of  claim 23 , wherein the polypeptide linker consists of an amino acid sequence selected from the group consisting of SEQ ID NOs: 51-54, 63-65 and 84. 
     
     
         30 . A composition comprising the polypeptide of  claim 23  and a carrier. 
     
     
         31 . A nucleic acid encoding a polypeptide of  claim 23 . 
     
     
         32 . A vector comprising the nucleic acid of  claim 31 . 
     
     
         33 . The vector of  claim 32 , which is an expression vector. 
     
     
         34 . A host cell comprising the vector of  claim 33 . 
     
     
         35 . A polypeptide comprising an immunoglobulin Fc domain comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 99-117, and a heterologous polypeptide, wherein the heterologous polypeptide is fused to the N-terminus or the C-terminus of the Fc domain by a polypeptide linker. 
     
     
         36 . The polypeptide of  claim 35 , wherein the polypeptide linker comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 51-54, 63-65 and 84.