Methods and apparatus for conducting multiple measurements on a sample

Abstract

Multiplexed test measurements are conducted using an assay module having a plurality of assay domains. In preferred embodiments, these measurements are conducted in assay modules having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.

1 .- 23 . (canceled) 
     
     
         24 . An assay module for measuring an enzymatic reaction comprising:
 (a) a first assay domain capable of binding to a substrate or product of said enzyme; and   (b) a second assay domain capable of binding to said enzyme.   
     
     
         25 . A method for measuring an enzymatic reaction comprising:
 (a) introducing an enzyme into an assay module comprising   (i) a first assay domain capable of binding one of a substrate or product of said enzyme; and   (ii) a second assay domain capable of binding said enzyme;   (b) binding said enzyme to said second assay domain;   (c) washing said assay module to purify said enzyme;   (d) contacting said enzyme with said substrate so as to form said product; and   (e) measuring the binding of said one of said substrate or product to said first assay domain so as to measure said enzymatic reaction.   
     
     
         26 . A method for measuring an enzymatic reaction comprising:
 (a) introducing an enzyme into an assay module comprising   (i) a first assay domain capable of binding one of a substrate or product of said enzyme; and   (ii) a second assay domain capable of binding said enzyme;   (b) binding said enzyme to said second assay domain to form an immobilized enzyme;   (c) contacting said enzyme with said substrate so as to form said product;   (d) measuring the binding of said one of said substrate or product to said first assay domain so as to measure said enzymatic reaction; and   (e) assaying for a modification of said immobilized enzyme.   
     
     
         27 . The method of  claim 26 , further comprising contacting said enzyme with a binding reagent specific for said modification. 
     
     
         28 . The method of  claim 26 , further comprising contacting said enzyme with a modifying activity, wherein said step of assaying for a modification measures said modifying activity. 
     
     
         29 . The assay module of  claim 24 , wherein said assay module is a multi-well plate and said assay domains in said assay module are located within a well of said plate. 
     
     
         30 . The multi-well plate of  claim 29 , wherein said well comprises one or more working electrode surfaces and said assay domains are located on said one or more working electrode surfaces. 
     
     
         31 . The assay module of  claim 24 , wherein said assay module further comprises one or more working electrode surfaces and said assay domains are located on said one or more working electrode surfaces. 
     
     
         32 . The method of  claim 25 , wherein said assay module is a multi-well plate and said assay domains in said assay module are located within a well of said plate. 
     
     
         33 . The method of  claim 26 , wherein said assay module is a multi-well plate and said assay domains in said assay module are located within a well of said plate. 
     
     
         34 . The method of  claim 32 , wherein said well comprises one or more working electrode surfaces and said assay domains are located on said one or more working electrode surfaces. 
     
     
         35 . The method of  claim 25 , wherein said assay module further comprises one or more working electrode surfaces and said assay domains are located on said one or more working electrode surfaces. 
     
     
         36 . The method of  claim 26 , wherein said assay module further comprises one or more working electrode surfaces and said assay domains are located on said one or more working electrode surfaces.