Methods and compositions for treatment of bone defects with placental cell populations

Abstract

Provided herein are methods of using adherent placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. Also provided herein are methods of differentiating the placental stem cells. Further provided herein are methods of using the placental stem cells to formulate implantable or injectable compositions suitable for administration to a subject. Still further provided herein are provides methods for treating bone defects with stem cells and compositions comprising stem cells.

1 - 4 . (canceled) 
     
     
         5 . A method for treating bone defects in a subject, comprising administering to a subject in need thereof an implantable or injectable composition comprising a population of nonadherent stem cells, thereby treating the bone defect in the subject. 
     
     
         6 . The method of  claim 5 , wherein the bone defect is an osteolytic lesion associated with a cancer, a bone fracture, or a spine in need of fusion. 
     
     
         7 . The method of  claim 6 , wherein the osteolytic lesion is associated with multiple myeloma, bone cancer, or metastatic cancer. 
     
     
         8 . The method of  claim 6 , wherein the bone fracture is a non-union fracture. 
     
     
         9 . The method of  claim 5 , wherein the implantable composition is surgically implanted. 
     
     
         10 . The method of  claim 5 , wherein an injectable composition comprising a population of nonadherent stem cells is administered to the subject. 
     
     
         11 . The method of  claim 5 , wherein the injectable composition is surgically administered to the region of the bone defect. 
     
     
         12 . The method of  claim 5 , wherein the injectable composition is systemically administered. 
     
     
         13 - 14 . (canceled) 
     
     
         15 . A method for formulating an injectable composition, comprising combining a population of stem cells with injectable hyaluronic acid or collagen, wherein said stem cells are CD200+ or HLA-G+, or are CD34+ and CD44−. 
     
     
         16 . A method for treating bone defects in a subject, comprising administering to a subject in need thereof an implantable or injectable composition comprising a population of stem cells, wherein said stem cells are CD200+ or HLA-G+, or are CD34+ and CD44−, thereby treating the bone defect in the subject. 
     
     
         17 . The method of  claim 15 , wherein the bone defect is an osteolytic lesion associated with multiple myeloma, bone cancer, or metastic cancer, a bone fracture, or a spine in need of fusion. 
     
     
         18 . The method of  claim 15 , wherein an implantable composition comprising a population of nonadherent stem cells is administered to the subject. 
     
     
         19 . The method of  claim 17 , wherein the implantable composition is surgically implanted. 
     
     
         20 . The method of  claim 15 , wherein an injectable composition comprising a population of nonadherent stem cells is administered to the subject. 
     
     
         21 . The method of  claim 19 , wherein the injectable composition is systemically administered.