US2019262504A1PendingUtilityA1

Bone marrow stem cell compositions, methods of making, and uses thereof

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Assignee: BIOLOGICA TECHPriority: Oct 12, 2015Filed: May 13, 2019Published: Aug 29, 2019
Est. expiryOct 12, 2035(~9.2 yrs left)· nominal 20-yr term from priority
Inventors:Amit Prakash Govil
A61P 19/00A61L 2300/414A61L 27/3604A61L 27/3895A61L 27/3834A61L 27/3683A61L 2430/02A61L 27/54A61K 38/18A61K 38/01A61L 2430/38A61L 27/3608A61L 27/3847
57
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Claims

Abstract

Provided herein are bone marrow stem cell compositions and implants and methods of making and using the same.

Claims

exact text as granted — not AI-modified
We claim: 
     
         1 . A bone marrow stem cell composition or implant comprising:
 viable bone marrow stem cells, wherein the bone marrow stem cell compositions is produced by the method comprising:   harvesting bone marrow from a donor;   selectively lysing the bone marrow to obtain bone marrow stem cells, wherein the step of selectively lysing is performed using water or a solution containing 0.5% or less v/v acetic acid.   
     
     
         2 . The bone marrow stem cell composition or implant of  claim 1 , wherein the method of producing the bone marrow stem cell composition or implant further comprises the step of washing the harvested bone marrow prior to selectively lysing the bone marrow. 
     
     
         3 . The bone marrow stem cell composition or implant of  claim 1 , wherein the method of producing the bone marrow stem cell composition or implant further comprises the step of centrifuging after the step of selectively lysing the bone marrow. 
     
     
         4 . The bone marrow stem cell composition or implant of  claim 1 , wherein the method of producing the bone marrow stem cell composition or implant further comprises the step of filtering after the step of selectively lysing the bone marrow. 
     
     
         5 . The bone marrow stem cell composition or implant of  claim 1 , wherein the method of producing the bone marrow stem cell composition or implant further comprises the step of adding a stabilization solution after the step of selectively lyisng the bone marrow. 
     
     
         6 . The bone marrow stem cell composition or implant of  claim 1 , wherein the composition or implant further comprises a scaffold material. 
     
     
         7 . The bone marrow stem cell composition or implant of  claim 6 , wherein the scaffold material is allograft bone. 
     
     
         8 . The bone marrow stem cell composition or implant of  claim 6 , wherein the scaffold material is allograft demineralized bone 
     
     
         9 . A method of making an bone marrow stem cells composition or implant containing viable stem cells, the method comprising:
 harvesting bone marrow from a donor;   selectively lysing the bone marrow to obtain bone marrow stem cells, wherein the step of selectively lysing is performed using water or a solution containing 0.5% or less v/v acetic acid.   
     
     
         10 . The method of  claim 9 , wherein the method further comprises the step of washing the harvested bone marrow prior to selectively lysing the bone marrow. 
     
     
         11 . The method of  claim 9 , wherein the method further comprises the step of centrifuging after the step of selectively lysing the bone marrow. 
     
     
         12 . The method of  claim 9 , wherein the method further comprises the step of filtering after the step of selectively lysing the bone marrow. 
     
     
         13 . The method of  claim 9 , wherein the method further comprises the step of adding a stabilization solution after the step of selectively lyisng the bone marrow. 
     
     
         14 . The method of  claim 9 , further comprising the step of mixing the bone marrow stem cell composition or implant with a scaffold material after the step of selectively lysing the bone marrow. 
     
     
         15 . The method of  claim 14 , wherein the scaffold material is allograft bone. 
     
     
         16 . The method of  claim 9 , further comprising the step of adding a scaffold material after the step of harvesting the bone marrow. 
     
     
         17 . The method of  claim 16 , wherein the scaffold material is allograft bone. 
     
     
         18 . A method of treating a subject in need thereof, the method comprising:
 administering an amount of a bone marrow stem cell composition or implant to a subject in need thereof wherein the bone marrow stem cell composition or implant is as according to  claim 1 .   
     
     
         19 . The method of  claim 18 , wherein the bone marrow stem cell composition or implant further comprises a scaffold and wherein the viable bone marrow stem cells are attached to or otherwise associated with the scaffold. 
     
     
         20 . The method of  claim 19 , wherein the scaffold is allograft bone.

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