A multi-component injection

Abstract

The invention relates to the technical field of medicines, and particularly relates to a multi-component injection and preparation processes and applications thereof. In the present invention, the effective ingredients of safflower carthamus. Red Paeony Root, Ligusticum wallichii, radix salviae miltiorrhizae and Angelica sinensis are analyzed and a multi-component injection is prepared via the modern pharmaceutical preparation technology. The study proves that the multi-component injection of the invention has therapeutic effects on Sepsis.

1 . A multi-component injection comprising the following ingredients, by weight: 8.66-35.26 parts of albiflorin std, 1000.0-1700.0 parts of paeoniflorin, 11.13-68.07 parts of Oxypaeoniflorin, 12.20-52.98 parts of Benzoylpaeoniflorin, 0.667-1.617 parts of Benzoyloxypaeoniflorin, 1.915-3.202 parts of Mudanpioside J, 10.63-20.13 parts of galloylpaeoniflorin, 0.804-1.338 parts of Mudanpioside C, 10.0-200.0 parts of benzoic acid, 0.21-15.88 parts of gallic acid, 0.2396-0.6860 parts of ethyl gallate, and 1.31-12.60 parts of catechin. 
     
     
         2 . The multi-component injection according to  claim 1 , which is prepared by a method comprising the following steps of:
 providing 100 g of Red Paeony Root decoction pieces; heating and boiling the Red Paeony Root decoction pieces with process water of 10 times the weight of the Red Paeony Root decoction pieces to obtain a first decoction after 2 hours slight boiling; filtering the first decoction to obtain filtrate I and a first dreg; boiling the first dreg with process water of 8 times the weight of the first dreg to obtain a second decoction after 1 hour slight boiling; filtering the second decoction to obtain filtrate II and a second dreg; mixing filtrate I and filtrate II to obtain a mixture; concentrating the mixture to obtain a first concentrate of 100 ml; adding a proper amount of gelatin solution to the first concentrate under stirring to obtain a gelatin-containing first concentrate; adding 95% ethanol to the gelatin-containing first concentrate to obtain an ethanol-containing first concentrate having an ethanol volume content of 70%; storing the ethanol-containing first concentrate under cooling condition for 24 hours to obtain a cool ethanol-containing first concentrate; filtering and concentrating the cool ethanol-containing first concentrate to obtain a second concentrate of 100 ml; extracting the second concentrate with water-saturated n-butanol for 4 times and using water-saturated n-butanol of 50 ml for each time; combining extract liquors of the 4 times extraction to obtain a combined extract liquor; recycling n-butanol from the combined extract liquor to obtain a n-butanol-reduced extract liquor without alcohol taste; and vacuum drying the n-butanol-reduced extract liquor to obtain a Red Paeony Root dry paste;   providing a proper amount of the Red Paeony Root dry paste; dissolving the Red Paeony Root dry paste in injection water to obtain a dilute of 200 ml; storing the dilute under cooling condition to obtain a first cool liquid; adding glucosum anhydricum of an amount in accordance with 4.5% mass fraction of the multi-component injection and injection water to the first cool liquid to obtain a 1000 ml liquid; adjusting the pH value of the 1000 ml liquid to 5.5-7.0 with a sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted liquid; storing the pH adjusted liquid under cooling condition to obtain a second cool liquid;   subjecting the second cool liquid to ultrafiltration to obtain an ultrafiltrate; adding a proper amount of solubilizing auxiliary materials which are dissolved in a proper amount of injection water into the ultrafiltrate to obtain a solubilizing auxiliary materials-containing ultrafiltrate; adjusting the pH value of the solubilizing auxiliary materials-containing ultrafiltrate to 5.5-7.0 using the sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted ultrafiltrate; filtering the pH adjusted ultrafiltrate to obtain a filtrate; encapsulating and sterilizing the filtrate to obtain the multi-component injection.   
     
     
         3 . A multi-component injection comprising the following ingredients, by weight: 8.66-35.26 parts of albiflorin std, 1000.0-1700.0 parts of paeoniflorin, 11.13-68.07 parts of Oxypaeoniflorin, 12.20-52.98 parts of Benzoylpaeoniflorin, 0.667-1.617 parts of Benzoyloxypaeoniflorin, 1.915-3.202 parts of Mudanpioside J, 10.63-20.13 parts of galloylpaeoniflorin, 0.804-1.338 parts of Mudanpioside C, 10.0-200.0 parts of benzoic acid, 0.21-15.88 parts of gallic acid, 0.2396-0.6860 parts of ethyl gallate, 1.31-12.60 parts of catechin, 1.232-3.547 parts of Kaempferol-3-0-glucoside, 0.0500-0.4184 parts of scutellarin, 0.755-2.570 parts of quercetin-3-0-glucoside, 8.42-29.40 parts of Kaempferol-3-0-rutinoside, 4.036-7.695 parts of Kaempferol-3-0-sophoroside, 1.517-5.598 parts of quercetin-3-0-rutinoside, 200.0-500.0 parts of Hydroxysafflor yellow A, 0.316-0.774 parts of uracil, 13.77-30.56 parts of adenine, 20.50-44.99 parts of phenylalanine, 11.44-27.13 parts of uridine, 5.07-12.63 parts of adenosine, 8.00-24.11 parts of guanosine, 4.96-16.86 parts of butanedioic acid, 2.384-5.404 parts of p-hydroxybenzoic acid, 3.00-17.98 parts of p-coumaric acid, 4.837-7.806 parts of caffeic acid, and 3.83-8.59 parts of chlorogenic acid. 
     
     
         4 . The multi-component injection according to  claim 3 , which is prepared by a method comprising the following steps of:
 providing 100 g of safflower carthamus decoction pieces; leaching the safflower carthamus decoction pieces with 30% ethanol of 8 times the weight of the safflower carthamus decoction pieces for 8 hours to obtain a leach liquor; filtering the leach liquor to obtain a liquid medicine of 4-6 times the weight of the safflower carthamus decoction pieces; adding 95% ethanol to the liquid medicine to obtain an ethanol-containing liquid medicine having an ethanol volume content of 70%; storing the ethanol-containing liquid medicine under cooling condition for 48 hours to obtain a cool ethanol-containing liquid medicine; filtering the cool ethanol-containing liquid medicine to obtain a first filtrate; concentrating the first filtrate under reduced pressure to obtain a concentrate of 100 ml; adding 95% ethanol to the concentrate to obtain an ethanol-containing concentrate having an ethanol volume content of 80%; storing the ethanol-containing concentrate under cooling condition for 48 hours to obtain a cool ethanol-containing concentrate;   filtering the cool ethanol-containing concentrate to obtain a second filtrate;   recycling ethanol from the second filtrate to obtain an ethanol-reduced filtrate;   concentrating and vacuum drying the ethanol-reduced filtrate to obtain a safflower carthamus dry paste;   providing 100 g of Red Paeony Root decoction pieces; heating and boiling the Red Paeony Root decoction pieces with process water of 10 times the weight of the Red Paeony Root decoction pieces to obtain a first decoction after 2 hours slight boiling; filtering the first decoction to obtain filtrate I and a first dreg; boiling the first dreg with process water of 8 times the weight of the first dreg to obtain a second decoction after 1 hour slight boiling; filtering the second decoction to obtain filtrate II and a second dreg; mixing filtrate I and filtrate II to obtain a mixture; concentrating the mixture to obtain a first concentrate of 100 ml; adding a proper amount of gelatin solution to the first concentrate under stirring to obtain a gelatin-containing first concentrate; adding 95% ethanol to the gelatin-containing first concentrate to obtain an ethanol-containing first concentrate having an ethanol volume content of 70%; storing the ethanol-containing first concentrate under cooling condition for 24 hours to obtain a cool ethanol-containing first concentrate; filtering and concentrating the cool ethanol-containing first concentrate to obtain a second concentrate of 100 ml; extracting the second concentrate with water-saturated n-butanol for 4 times and using 50 ml water-saturated n-butanol for each time; combining extract liquors of the 4 times extraction to obtain a combined extract liquor; recycling n-butanol from the combined extract liquor to obtain a n-butanol-reduced extract liquor without alcohol taste; and vacuum drying the n-butanol-reduced extract liquor to obtain a Red Paeony Root dry paste;   providing the safflower carthamus dry paste and the Red Paeony Root dry paste each of a proper amount; dissolving the two dry pastes in injection water to obtain a dilute of 200 ml; storing the dilute under cooling condition to obtain a first cool liquid; adding glucosum anhydricum of an amount in accordance with 4.5% mass fraction of the multi-component injection and injection water to the first cool liquid to obtain a 1000 ml liquid; adjusting the pH value of the 1000 ml liquid to 5.5-7.0 with a sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted liquid; storing the pH adjusted liquid under cooling condition to obtain a second cool liquid; subjecting the second cool liquid to ultrafiltration to obtain an ultrafiltrate; adding a proper amount of solubilizing auxiliary materials which are dissolved in a proper amount of injection water into the ultrafiltrate to obtain a solubilizing auxiliary materials-containing ultrafiltrate; adjusting the pH value of the solubilizing auxiliary materials-containing ultrafiltrate to 5.5-7.0 using the sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted ultrafiltrate; filtering the pH adjusted ultrafiltrate to obtain a filtrate; encapsulating and sterilizing the filtrate to obtain the multi-component injection.   
     
     
         5 . A multi-component injection comprising the following ingredients, by weight: 8.66-35.26 parts of albiflorin std, 1000.0-1700.0 parts of paeoniflorin, 11.13-68.07 parts of Oxypaeoniflorin, 12.20-52.98 parts of Benzoylpaeoniflorin, 0.667-1.617 parts of Benzoyloxypaeoniflorin, 1.915-3.202 parts of Mudanpioside J, 10.63-20.13 parts of galloylpaeoniflorin, 0.804-1.338 parts of Mudanpioside C, 10.0-200.0 parts of benzoic acid, 0.21-15.88 parts of gallic acid, 0.2396-0.6860 parts of ethyl gallate, 1.31-12.60 parts of catechin, 1.232-3.547 parts of Kaempferol-3-0-glucoside, 0.0500-0.4184 parts of scutellarin, 0.755-2.570 parts of quercetin-3-0-glucoside, 8.42-29.40 parts of Kaempferol-3-0-rutinoside, 4.036-7.695 parts of Kaempferol-3-0-sophoroside, 1.517-5.598 parts of quercetin-3-0-rutinoside, 200.0-500.0 parts of Hydroxysafflor yellow A, 0.316-0.774 parts of uracil, 13.77-30.56 parts of adenine, 20.50-44.99 parts of phenylalanine, 11.44-27.13 parts of uridine, 5.07-12.63 parts of adenosine, 8.00-24.11 parts of guanosine, 4.96-16.86 parts of butanedioic acid, 2.384-5.404 parts of p-hydroxybenzoic acid, 3.00-17.98 parts of p-coumaric acid, 4.837-7.806 parts of caffeic acid, 3.83-8.59 parts of chlorogenic acid, 6.51-69.39 parts of senkyunolide I (42), 1.55-18.27 parts of senkyunolide H, 4.30-14.22 parts of senkyunolide N, 2.460-5.648 parts of open-loop senkyunolide I, 1.55-10.74 parts of senkyunolide G, 1.43-8.67 parts of 3-hydroxyl-3-butylphthalide, 0.10-0.961 parts of senkyunolide A, and 7.66-47.15 parts of ferulaic acid. 
     
     
         6 . The multi-component injection according to  claim 5 , which is prepared by a method comprising the following steps of:
 providing 100 g of safflower carthamus decoction pieces; leaching the safflower carthamus decoction pieces with 30% ethanol of 8 times the weight of the safflower carthamus decoction pieces for 8 hours to obtain a leach liquor; filtering the leach liquor to obtain a liquid medicine of 4-6 times the weight of the safflower carthamus decoction pieces; adding 95% ethanol to the liquid medicine to obtain an ethanol-containing liquid medicine having an ethanol volume content of 70%; storing the ethanol-containing liquid medicine under cooling condition for 48 hours to obtain a cool ethanol-containing liquid medicine; filtering the cool ethanol-containing liquid medicine to obtain a first filtrate; concentrating the first filtrate under reduced pressure to obtain a concentrate of 100 ml; adding 95% ethanol to the concentrate to obtain an ethanol-containing concentrate having an ethanol volume content of 80%; storing the ethanol-containing concentrate under cooling condition for 48 hours to obtain a cool ethanol-containing concentrate;   filtering the cool ethanol-containing concentrate to obtain a second filtrate;   recycling ethanol from the second filtrate to obtain an ethanol-reduced filtrate;   concentrating and vacuum drying the ethanol-reduced filtrate to obtain a safflower carthamus dry paste;   providing 100 g of Red Paeony Root decoction pieces; heating and boiling the Red Paeony Root decoction pieces with process water of 10 times the weight of the Red Paeony Root decoction pieces to obtain a first decoction after 2 hours slight boiling; filtering the first decoction to obtain filtrate I and a first dreg; boiling the first dreg with process water of 8 times the weight of the first dreg to obtain a second decoction after 1 hour slight boiling; filtering the second decoction to obtain filtrate II and a second dreg; mixing filtrate I and filtrate II to obtain a mixture; concentrating the mixture to obtain a first concentrate of 100 ml; adding a proper amount of gelatin solution to the first concentrate under stirring to obtain a gelatin-containing first concentrate; adding 95% ethanol to the gelatin-containing first concentrate to obtain an ethanol-containing first concentrate having an ethanol volume content of 70%; storing the ethanol-containing first concentrate under cooling condition for 24 hours to obtain a cool ethanol-containing first concentrate; filtering and concentrating the cool ethanol-containing first concentrate to obtain a second concentrate of 100 ml; extracting the second concentrate with water-saturated n-butanol for 4 times and using 50 ml water-saturated n-butanol for each time; combining extract liquors of the 4 times extraction to obtain a combined extract liquor; recycling n-butanol from the combined extract liquor to obtain a n-butanol-reduced extract liquor without alcohol taste; and vacuum drying the n-butanol-reduced extract liquor to obtain a Red Paeony Root dry paste;   providing 100 g of  Ligusticum wallichii  decoction pieces and 100 g of  Angelica sinensis  decoction pieces; treating the  Ligusticum wallichii  decoction pieces and  Angelica sinensis  decoction pieces with a same treatment process as the Red Paeony Root decoction pieces except for keeping 300 ml of concentrate every time and extracting with 150 ml of water-saturated n-butanol each time, to obtain a third dry paste;   providing the safflower carthamus dry paste, the Red Paeony Root dry paste and the third dry paste each of a proper amount; dissolving the three dry pastes in injection water to obtain a dilute of 200 ml; storing the dilute under cooling condition to obtain a first cool liquid; adding glucosum anhydricum of an amount in accordance with 4.5% mass fraction of the multi-component injection and injection water to the first cool liquid to obtain a 1000 ml liquid; adjusting the pH value of the 1000 ml liquid to 5.5-7.0 with a sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted liquid; storing the pH adjusted liquid under cooling condition to obtain a second cool liquid; subjecting the second cool liquid to ultrafiltration to obtain an ultrafiltrate; adding a proper amount of solubilizing auxiliary materials which are dissolved in a proper amount of injection water into the ultrafiltrate to obtain a solubilizing auxiliary materials-containing ultrafiltrate;   adjusting the pH value of the solubilizing auxiliary materials-containing ultrafiltrate to 5.5-7.0 using the sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted ultrafiltrate; filtering the pH adjusted ultrafiltrate to obtain a filtrate;   encapsulating and sterilizing the filtrate to obtain the multi-component injection.   
     
     
         7 . A multi-component injection comprising the following ingredients, by weight: 8.66-35.26 parts of albiflorin std, 1000.0-1700.0 parts of paeoniflorin, 11.13-68.07 parts of Oxypaeoniflorin, 12.20-52.98 parts of Benzoylpaeoniflorin, 0.667-1.617 parts of Benzoyloxypaeoniflorin, 1.915-3.202 parts of Mudanpioside J, 10.63-20.13 parts of galloylpaeoniflorin, 0.804-1.338 parts of Mudanpioside C, 10.0-200.0 parts of benzoic acid, 0.21-15.88 parts of gallic acid, 0.2396-0.6860 parts of ethyl gallate, 1.31-12.60 parts of catechin, 1.232-3.547 parts of Kaempferol-3-0-glucoside, 0.0500-0.4184 parts of scutellarin, 0.755-2.570 parts of quercetin-3-0-glucoside, 8.42-29.40 parts of Kaempferol-3-0-rutinoside, 4.036-7.695 parts of Kaempferol-3-0-sophoroside, 1.517-5.598 parts of quercetin-3-0-rutinoside, 200.0-500.0 parts of Hydroxysafflor yellow A, 0.316-0.774 parts of uracil, 13.77-30.56 parts of adenine, 20.50-44.99 parts of phenylalanine, 11.44-27.13 parts of uridine, 5.07-12.63 parts of adenosine, 8.00-24.11 parts of guanosine, 4.96-16.86 parts of butanedioic acid, 2.384-5.404 parts of p-hydroxybenzoic acid, 3.00-17.98 parts of p-coumaric acid, 4.837-7.806 parts of caffeic acid, 3.83-8.59 parts of chlorogenic acid, 6.51-69.39 parts of senkyunolide I (42), 1.55-18.27 parts of senkyunolide H, 4.30-14.22 parts of senkyunolide N, 2.460-5.648 parts of open-loop senkyunolide I, 1.55-10.74 parts of senkyunolide G, 1.43-8.67 parts of 3-hydroxyl-3-butylphthalide, 0.10-0.961 parts of senkyunolide A, 7.66-47.15 parts of ferulaic acid, 1.43-17.25 parts of protocatechualdehyde, 2.361-4.030 parts of protocatechuic acid, 2.776-6.845 parts of tanshinol, 5.07-12.78 parts of rosmarinic acid, 0.0697-0.4005 parts of salvianolic acid D, 1.123-4.732 parts of salvianolic acid C, 0.366-2.505 parts of salvianolic acid A, 0.429-0.945 parts of alkannic acid, and 4.00-11.00 parts of salvianolic acid B. 
     
     
         8 . The multi-component injection according to  claim 7 , which is prepared by a method comprising the following steps:
 providing 100 g of safflower carthamus decoction pieces; leaching the safflower carthamus decoction pieces with 30% ethanol of 8 times the weight of the safflower carthamus decoction pieces for 8 hours to obtain a leach liquor; filtering the leach liquor to obtain a liquid medicine of 4-6 times the weight of the safflower carthamus decoction pieces; adding 95% ethanol to the liquid medicine to obtain an ethanol-containing liquid medicine having an ethanol volume content of 70%; storing the ethanol-containing liquid medicine under cooling condition for 48 hours to obtain a cool ethanol-containing liquid medicine; filtering the cool ethanol-containing liquid medicine to obtain a first filtrate; concentrating the first filtrate under reduced pressure to obtain a concentrate of 100 ml; adding 95% ethanol to the concentrate to obtain an ethanol-containing concentrate having an ethanol volume content of 80%; storing the ethanol-containing concentrate under cooling condition for 48 hours to obtain a cool ethanol-containing concentrate;   filtering the cool ethanol-containing concentrate to obtain a second filtrate;   recycling ethanol from the second filtrate to obtain an ethanol-reduced filtrate;   concentrating and vacuum drying the ethanol-reduced filtrate to obtain a safflower carthamus dry paste;   providing 100 g of Red Paeony Root decoction pieces; heating and boiling the Red Paeony Root decoction pieces with process water of 10 times the weight of the Red Paeony Root decoction pieces to obtain a first decoction after 2 hours slight boiling; filtering the first decoction to obtain filtrate I and a first dreg; boiling the first dreg with process water of 8 times the weight of the first dreg to obtain a second decoction after 1 hour slight boiling; filtering the second decoction to obtain filtrate II and a second dreg; mixing filtrate I and filtrate II to obtain a mixture; concentrating the mixture to obtain a first concentrate of 100 ml; adding a proper amount of gelatin solution to the first concentrate under stirring to obtain a gelatin-containing first concentrate; adding 95% ethanol to the gelatin-containing first concentrate to obtain an ethanol-containing first concentrate having an ethanol volume content of 70%; storing the ethanol-containing first concentrate under cooling condition for 24 hours to obtain a cool ethanol-containing first concentrate; filtering and concentrating the cool ethanol-containing first concentrate to obtain a second concentrate of 100 ml; extracting the second concentrate with water-saturated n-butanol for 4 times and using 50 ml water-saturated n-butanol for each time; combining extract liquors of the 4 times extraction to obtain a combined extract liquor; recycling n-butanol from the combined extract liquor to obtain a n-butanol-reduced extract liquor without alcohol taste; and vacuum drying the n-butanol-reduced extract liquor to obtain a Red Paeony Root dry paste;   providing 100 g of  Ligusticum wallichii  decoction pieces, 100 g of radix salviae miltiorrhizae and 100 g of  Angelica sinensis  decoction pieces; treating the three decoction pieces with a same treatment process as the Red Paeony Root decoction pieces except for keeping 300 ml of concentrate every time and extracting with 150 ml of water-saturated n-butanol each time, to obtain a third dry paste;   providing the safflower carthamus dry paste, the Red Paeony Root dry paste and the third dry paste each of a proper amount; dissolving the three dry pastes in injection water to obtain a dilute of 200 ml; storing the dilute under cooling condition to obtain a first cool liquid; adding glucosum anhydricum of an amount in accordance with 4.5% mass fraction of the multi-component injection and injection water to the first cool liquid to obtain a 1000 ml liquid; adjusting the pH value of the 1000 ml liquid to 5.5-7.0 with a sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted liquid; storing the pH adjusted liquid under cooling condition to obtain a second cool liquid; subjecting the second cool liquid to ultrafiltration to obtain an ultrafiltrate; adding a proper amount of solubilizing auxiliary materials which are dissolved in a proper amount of injection water into the ultrafiltrate to obtain a solubilizing auxiliary materials-containing ultrafiltrate;   adjusting the pH value of the solubilizing auxiliary materials-containing ultrafiltrate to 5.5-7.0 using the sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted ultrafiltrate; filtering the pH adjusted ultrafiltrate to obtain a filtrate;   encapsulating and sterilizing the filtrate to obtain the multi-component injection.   
     
     
         9 . The multi-component injection according to  claim 1 , wherein the multi-component injection is administered via an injection. 
     
     
         10 . A method for treating sepsis in a subject in need thereof comprising administering to the subject the multi-component injection according to  claim 1 . 
     
     
         11 . The multi-component injection according to  claim 3 , wherein the multi-component injection is administered via an injection. 
     
     
         12 . The multi-component injection according to  claim 5 , wherein the multi-component injection is administered via an injection. 
     
     
         13 . The multi-component injection according to  claim 7 , wherein the multi-component injection is administered via an injection. 
     
     
         14 . A method for treating sepsis in a subject in need thereof comprising administering to the subject the multi-component injection according to  claim 3 . 
     
     
         15 . A method for treating sepsis in a subject in need thereof comprising administering to the subject the multi-component injection according to  claim 5 . 
     
     
         16 . A method for treating sepsis in a subject in need thereof comprising administering to the subject the multi-component injection according to  claim 7 . 
     
     
         17 . A method of preparing a multi-component injection of  claim 1  comprising the following steps:
 providing 100 g of Red Paeony Root decoction pieces; heating and boiling the Red Paeony Root decoction pieces with process water of 10 times the weight of the Red Paeony Root decoction pieces to obtain a first decoction after 2 hours slight boiling; 
 filtering the first decoction to obtain filtrate I and a first dreg; boiling the first dreg with process water of 8 times the weight of the first dreg to obtain a second decoction after 1 hour slight boiling; filtering the second decoction to obtain filtrate II and a second dreg; mixing filtrate I and filtrate II to obtain a mixture; concentrating the mixture to obtain a first concentrate of 100 ml; adding a proper amount of gelatin solution to the first concentrate under stirring to obtain a gelatin-containing first concentrate; adding 95% ethanol to the gelatin-containing first concentrate to obtain an ethanol-containing first concentrate having an ethanol volume content of 70%; 
 storing the ethanol-containing first concentrate under cooling condition for 24 hours to obtain a cool ethanol-containing first concentrate; filtering and concentrating the cool ethanol-containing first concentrate to obtain a second concentrate of 100 ml; 
 extracting the second concentrate with water-saturated n-butanol for 4 times and using water-saturated n-butanol of 50 ml for each time; combining extract liquors of the 4 times extraction to obtain a combined extract liquor; recycling n-butanol from the combined extract liquor to obtain a n-butanol-reduced extract liquor without alcohol taste; and vacuum drying the n-butanol-reduced extract liquor to obtain a Red Paeony Root dry paste; 
 providing a proper amount of the Red Paeony Root dry paste; dissolving the Red Paeony Root dry paste in injection water to obtain a dilute of 200 ml; storing the dilute under cooling condition to obtain a first cool liquid; adding glucosum anhydricum of an amount in accordance with 4.5% mass fraction of the multi-component injection and injection water to the first cool liquid to obtain a 1000 ml liquid; adjusting the pH value of the 1000 ml liquid to 5.5-7.0 with a sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted liquid; storing the pH adjusted liquid under cooling condition to obtain a second cool liquid; subjecting the second cool liquid to ultrafiltration to obtain an ultrafiltrate; adding a proper amount of solubilizing auxiliary materials which are dissolved in a proper amount of injection water into the ultrafiltrate to obtain a solubilizing auxiliary materials-containing ultrafiltrate; adjusting the pH value of the solubilizing auxiliary materials-containing ultrafiltrate to 5.5-7.0 using the sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted ultrafiltrate; filtering the pH adjusted ultrafiltrate to obtain a filtrate; encapsulating and sterilizing the filtrate to obtain the multi-component injection. 
 
     
     
         18 . A method of preparing a multi-component injection of  claim 3  comprising the following steps:
 providing 100 g of safflower carthamus decoction pieces; leaching the safflower carthamus decoction pieces with 30% ethanol of 8 times the weight of the safflower carthamus decoction pieces for 8 hours to obtain a leach liquor; filtering the leach liquor to obtain a liquid medicine of 4-6 times the weight of the safflower carthamus decoction pieces; adding 95% ethanol to the liquid medicine to obtain an ethanol-containing liquid medicine having an ethanol volume content of 70%; storing the ethanol-containing liquid medicine under cooling condition for 48 hours to obtain a cool ethanol-containing liquid medicine; filtering the cool ethanol-containing liquid medicine to obtain a first filtrate; concentrating the first filtrate under reduced pressure to obtain a concentrate of 100 ml; adding 95% ethanol to the concentrate to obtain an ethanol-containing concentrate having an ethanol volume content of 80%; storing the ethanol-containing concentrate under cooling condition for 48 hours to obtain a cool ethanol-containing concentrate; filtering the cool ethanol-containing concentrate to obtain a second filtrate; recycling ethanol from the second filtrate to obtain an ethanol-reduced filtrate; concentrating and vacuum drying the ethanol-reduced filtrate to obtain a safflower carthamus dry paste; 
 providing 100 g of Red Paeony Root decoction pieces; heating and boiling the Red Paeony Root decoction pieces with process water of 10 times the weight of the Red Paeony Root decoction pieces to obtain a first decoction after 2 hours slight boiling; 
 filtering the first decoction to obtain filtrate I and a first dreg; boiling the first dreg with process water of 8 times the weight of the first dreg to obtain a second decoction after 1 hour slight boiling; filtering the second decoction to obtain filtrate II and a second dreg; mixing filtrate I and filtrate II to obtain a mixture; concentrating the mixture to obtain a first concentrate of 100 ml; adding a proper amount of gelatin solution to the first concentrate under stirring to obtain a gelatin-containing first concentrate; adding 95% ethanol to the gelatin-containing first concentrate to obtain an ethanol-containing first concentrate having an ethanol volume content of 70%; 
 storing the ethanol-containing first concentrate under cooling condition for 24 hours to obtain a cool ethanol-containing first concentrate; filtering and concentrating the cool ethanol-containing first concentrate to obtain a second concentrate of 100 ml; 
 extracting the second concentrate with water-saturated n-butanol for 4 times and using 50 ml water-saturated n-butanol for each time; combining extract liquors of the 4 times extraction to obtain a combined extract liquor; recycling n-butanol from the combined extract liquor to obtain a n-butanol-reduced extract liquor without alcohol taste; and vacuum drying the n-butanol-reduced extract liquor to obtain a Red Paeony Root dry paste; 
 providing the safflower carthamus dry paste and the Red Paeony Root dry paste each of a proper amount; dissolving the two dry pastes in injection water to obtain a dilute of 200 ml; storing the dilute under cooling condition to obtain a first cool liquid; 
 adding glucosum anhydricum of an amount in accordance with 4.5% mass fraction of the multi-component injection and injection water to the first cool liquid to obtain a 1000 ml liquid; adjusting the pH value of the 1000 ml liquid to 5.5-7.0 with a sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted liquid; storing the pH adjusted liquid under cooling condition to obtain a second cool liquid; subjecting the second cool liquid to ultrafiltration to obtain an ultrafiltrate; adding a proper amount of solubilizing auxiliary materials which are dissolved in a proper amount of injection water into the ultrafiltrate to obtain a solubilizing auxiliary materials-containing ultrafiltrate; adjusting the pH value of the solubilizing auxiliary materials-containing ultrafiltrate to 5.5-7.0 using the sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted ultrafiltrate; filtering the pH adjusted ultrafiltrate to obtain a filtrate; encapsulating and sterilizing the filtrate to obtain the multi-component injection. 
 
     
     
         19 . A method of preparing a multi-component injection of  claim 5  comprising the following steps:
 providing 100 g of safflower carthamus decoction pieces; leaching the safflower carthamus decoction pieces with 30% ethanol of 8 times the weight of the safflower carthamus decoction pieces for 8 hours to obtain a leach liquor; filtering the leach liquor to obtain a liquid medicine of 4-6 times the weight of the safflower carthamus decoction pieces; adding 95% ethanol to the liquid medicine to obtain an ethanol-containing liquid medicine having an ethanol volume content of 70%; storing the ethanol-containing liquid medicine under cooling condition for 48 hours to obtain a cool ethanol-containing liquid medicine; filtering the cool ethanol-containing liquid medicine to obtain a first filtrate; concentrating the first filtrate under reduced pressure to obtain a concentrate of 100 ml; adding 95% ethanol to the concentrate to obtain an ethanol-containing concentrate having an ethanol volume content of 80%; storing the ethanol-containing concentrate under cooling condition for 48 hours to obtain a cool ethanol-containing concentrate; filtering the cool ethanol-containing concentrate to obtain a second filtrate; recycling ethanol from the second filtrate to obtain an ethanol-reduced filtrate; concentrating and vacuum drying the ethanol-reduced filtrate to obtain a safflower carthamus dry paste; 
 providing 100 g of Red Paeony Root decoction pieces; heating and boiling the Red Paeony Root decoction pieces with process water of 10 times the weight of the Red Paeony Root decoction pieces to obtain a first decoction after 2 hours slight boiling; 
 filtering the first decoction to obtain filtrate I and a first dreg; boiling the first dreg with process water of 8 times the weight of the first dreg to obtain a second decoction after 1 hour slight boiling; filtering the second decoction to obtain filtrate II and a second dreg; mixing filtrate I and filtrate II to obtain a mixture; concentrating the mixture to obtain a first concentrate of 100 ml; adding a proper amount of gelatin solution to the first concentrate under stirring to obtain a gelatin-containing first concentrate; adding 95% ethanol to the gelatin-containing first concentrate to obtain an ethanol-containing first concentrate having an ethanol volume content of 70%; 
 storing the ethanol-containing first concentrate under cooling condition for 24 hours to obtain a cool ethanol-containing first concentrate; filtering and concentrating the cool ethanol-containing first concentrate to obtain a second concentrate of 100 ml; 
 extracting the second concentrate with water-saturated n-butanol for 4 times and using 50 ml water-saturated n-butanol for each time; combining extract liquors of the 4 times extraction to obtain a combined extract liquor; recycling n-butanol from the combined extract liquor to obtain a n-butanol-reduced extract liquor without alcohol taste; and vacuum drying the n-butanol-reduced extract liquor to obtain a Red Paeony Root dry paste; 
 providing 100 g of  Ligusticum wallichii  decoction pieces and 100 g of  Angelica sinensis  decoction pieces; treating the  Ligusticum wallichii  decoction pieces and  Angelica sinensis  decoction pieces with a same treatment process as the Red Paeony Root decoction pieces except for keeping 300 ml of concentrate every time and extracting with 150 ml of water-saturated n-butanol each time, to obtain a third dry paste; 
 providing the safflower carthamus dry paste, the Red Paeony Root dry paste and the third dry paste each of a proper amount; dissolving the three dry pastes in injection water to obtain a dilute of 200 ml; storing the dilute under cooling condition to obtain a first cool liquid; adding glucosum anhydricum of an amount in accordance with 4.5% mass fraction of the multi-component injection and injection water to the first cool liquid to obtain a 1000 ml liquid; adjusting the pH value of the 1000 ml liquid to 5.5-7.0 with a sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted liquid; storing the pH adjusted liquid under cooling condition to obtain a second cool liquid; subjecting the second cool liquid to ultrafiltration to obtain an ultrafiltrate; adding a proper amount of solubilizing auxiliary materials which are dissolved in a proper amount of injection water into the ultrafiltrate to obtain a solubilizing auxiliary materials-containing ultrafiltrate; adjusting the pH value of the solubilizing auxiliary materials-containing ultrafiltrate to 5.5-7.0 using the sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted ultrafiltrate; filtering the pH adjusted ultrafiltrate to obtain a filtrate; encapsulating and sterilizing the filtrate to obtain the multi-component injection. 
 
     
     
         20 . A method of preparing a multi-component injection of  claim 7  comprising the following steps:
 providing 100 g of safflower carthamus decoction pieces; leaching the safflower carthamus decoction pieces with 30% ethanol of 8 times the weight of the safflower carthamus decoction pieces for 8 hours to obtain a leach liquor; filtering the leach liquor to obtain a liquid medicine of 4-6 times the weight of the safflower carthamus decoction pieces; adding 95% ethanol to the liquid medicine to obtain an ethanol-containing liquid medicine having an ethanol volume content of 70%; storing the ethanol-containing liquid medicine under cooling condition for 48 hours to obtain a cool ethanol-containing liquid medicine; filtering the cool ethanol-containing liquid medicine to obtain a first filtrate; concentrating the first filtrate under reduced pressure to obtain a concentrate of 100 ml; adding 95% ethanol to the concentrate to obtain an ethanol-containing concentrate having an ethanol volume content of 80%; storing the ethanol-containing concentrate under cooling condition for 48 hours to obtain a cool ethanol-containing concentrate; filtering the cool ethanol-containing concentrate to obtain a second filtrate; recycling ethanol from the second filtrate to obtain an ethanol-reduced filtrate; concentrating and vacuum drying the ethanol-reduced filtrate to obtain a safflower carthamus dry paste; 
 providing 100 g of Red Paeony Root decoction pieces; heating and boiling the Red Paeony Root decoction pieces with process water of 10 times the weight of the Red Paeony Root decoction pieces to obtain a first decoction after 2 hours slight boiling; 
 filtering the first decoction to obtain filtrate I and a first dreg; boiling the first dreg with process water of 8 times the weight of the first dreg to obtain a second decoction after 1 hour slight boiling; filtering the second decoction to obtain filtrate II and a second dreg; mixing filtrate I and filtrate II to obtain a mixture; concentrating the mixture to obtain a first concentrate of 100 ml; adding a proper amount of gelatin solution to the first concentrate under stirring to obtain a gelatin-containing first concentrate; adding 95% ethanol to the gelatin-containing first concentrate to obtain an ethanol-containing first concentrate having an ethanol volume content of 70%; 
 storing the ethanol-containing first concentrate under cooling condition for 24 hours to obtain a cool ethanol-containing first concentrate; filtering and concentrating the cool ethanol-containing first concentrate to obtain a second concentrate of 100 ml; 
 extracting the second concentrate with water-saturated n-butanol for 4 times and using 50 ml water-saturated n-butanol for each time; combining extract liquors of the 4 times extraction to obtain a combined extract liquor; recycling n-butanol from the combined extract liquor to obtain a n-butanol-reduced extract liquor without alcohol taste; and vacuum drying the n-butanol-reduced extract liquor to obtain a Red Paeony Root dry paste; 
 providing 100 g of  Ligusticum wallichii  decoction pieces, 100 g of radix salviae miltiorrhizae and 100 g of  Angelica sinensis  decoction pieces; treating the three decoction pieces with a same treatment process as the Red Paeony Root decoction pieces except for keeping 300 ml of concentrate every time and extracting with 150 ml of water-saturated n-butanol each time, to obtain a third dry paste; 
 providing the safflower carthamus dry paste, the Red Paeony Root dry paste and the third dry paste each of a proper amount; dissolving the three dry pastes in injection water to obtain a dilute of 200 ml; storing the dilute under cooling condition to obtain a first cool liquid; adding glucosum anhydricum of an amount in accordance with 4.5% mass fraction of the multi-component injection and injection water to the first cool liquid to obtain a 1000 ml liquid; adjusting the pH value of the 1000 ml liquid to 5.5-7.0 with a sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted liquid; storing the pH adjusted liquid under cooling condition to obtain a second cool liquid; subjecting the second cool liquid to ultrafiltration to obtain an ultrafiltrate; adding a proper amount of solubilizing auxiliary materials which are dissolved in a proper amount of injection water into the ultrafiltrate to obtain a solubilizing auxiliary materials-containing ultrafiltrate; adjusting the pH value of the solubilizing auxiliary materials-containing ultrafiltrate to 5.5-7.0 using the sodium hydroxide solution of 10% mass fraction to obtain a pH adjusted ultrafiltrate; filtering the pH adjusted ultrafiltrate to obtain a filtrate; encapsulating and sterilizing the filtrate to obtain the multi-component injection.