Programmable immunocyte receptor complex system

Abstract

A programmable receptor complex expressed by an immunocyte, wherein the programmable receptor complex includes a plurality of native or endogenously-expressed receptor subunits, wherein at least one of the plurality of native or endogenously-expressed receptor subunits has been engineered or modified to include an FcγRI receptor component or a biotin-binding component, and wherein the FcγRI receptor component or biotin-binding component is operative to bind to a target detector molecule that binds to or otherwise interacts with a predetermined target.

What is claimed: 
     
         1 . A programmable receptor complex expressed by an immunocyte, wherein the programmable receptor complex includes a plurality of native or endogenously-expressed receptor subunits, wherein at least one of the plurality of native or endogenously-expressed receptor subunits has been engineered or modified to include a biotin-binding component, and wherein the biotin-binding component is operative to bind to a target detector molecule that binds to or otherwise interacts with a predetermined target. 
     
     
         2 . The programmable immunocyte receptor complex of  claim 1 , wherein the plurality of native or endogenously-expressed receptor subunits are T cell receptor subunits. 
     
     
         3 . The programmable immunocyte receptor complex of  claim 2 , wherein the plurality of native or endogenously-expressed receptor subunits includes at least one CD3-delta, CD3-gamma, TCR alpha, TCR beta, two CD3-zeta and two CD3-epsilon. 
     
     
         4 . The programmable immunocyte receptor complex of  claim 3 , wherein the native or endogenously-expressed receptor subunit that has been engineered or modified to include a biotin-binding component is the CD3-epsilon subunit. 
     
     
         5 . The programmable immunocyte receptor complex of  claim 1 , wherein the immunocyte is a CD4+ T cell, a CD8+ T cell, γδ T cell, or allogenic cell. 
     
     
         6 . The programmable immunocyte receptor complex of  claim 1 , wherein the biotin-binding component is monomeric streptavidin 2 or enhanced monoavidin. 
     
     
         7 . The programmable immunocyte receptor complex of  claim 1 , wherein the biotin-binding component is chicken avidin. 
     
     
         8 . The programmable immunocyte receptor complex cell system of  claim 1 , wherein the target detector molecule includes a biotin moiety, a stabilizing core structure, and a paratope or other ligand that is specific for the predetermined target. 
     
     
         9 . The programmable immunocyte receptor complex of  claim 1 , wherein the predetermined target is a cancer cell or cancer cell determinant of a known type, or an infectious disease agent or a determinant of an infectious disease agent of a known type. 
     
     
         10 . A programmable immunocyte receptor complex cell system, comprising:
 (a) an immunocyte; and   (b) a programmable receptor complex expressed by the immunocyte,
 (i) wherein the programmable receptor complex includes a plurality of native or endogenously-expressed receptor subunits, 
 (ii) wherein at least one of the plurality of native or endogenously-expressed receptor subunits has been engineered or modified to include a biotin-binding component, and 
 (iii) wherein the biotin-binding component is operative to bind to a target detector molecule that binds to or otherwise interacts with a predetermined target. 
   
     
     
         11 . The programmable immunocyte receptor complex cell system of  claim 10 , wherein the plurality of native or endogenously-expressed receptor subunits are T cell receptor subunits. 
     
     
         12 . The programmable immunocyte receptor complex cell system of  claim 11 , wherein the plurality of native or endogenously-expressed receptor subunits includes at least one CD3-delta, CD3-gamma, TCR alpha, TCR beta, two CD3-zeta and two CD3-epsilon. 
     
     
         13 . The programmable immunocyte receptor complex cell system of  claim 12 , wherein the native or endogenously-expressed receptor subunit that has been engineered or modified to include a biotin-binding component is the CD3-epsilon subunit. 
     
     
         14 . The programmable immunocyte receptor complex cell system of  claim 10 , wherein the immunocyte is a CD4+ T cell, a CD8+ T cell, γδ T cell, or allogenic cell. 
     
     
         15 . The programmable immunocyte receptor complex cell system of  claim 10 , wherein the biotin-binding component is monomeric streptavidin 2 or enhanced monoavidin. 
     
     
         16 . The programmable immunocyte receptor complex cell system of  claim 10 , wherein the biotin-binding component is chicken avidin. 
     
     
         17 . The programmable immunocyte receptor complex cell system of  claim 10 , wherein the target detector molecule includes a biotin moiety, a stabilizing core structure, and a paratope or other ligand that is specific for the predetermined target. 
     
     
         18 . The programmable immunocyte receptor complex cell system of  claim 10 , wherein the predetermined target is a cancer cell or cancer cell determinant of a known type, or an infectious disease agent or a determinant of an infectious disease agent of a known type. 
     
     
         19 . The programmable immunocyte receptor complex cell system of  claim 10 , wherein the system is adapted for diagnostic use. 
     
     
         20 . The programmable immunocyte receptor complex cell system of  claim 10 , wherein the system is adapted for therapeutic use. 
     
     
         21 . A programmable immunocyte receptor complex cell system, comprising:
 (a) an immunocyte; and   (b) a programmable receptor complex expressed by the immunocyte,
 (i) wherein the programmable receptor complex includes a plurality of native or endogenously-expressed receptor subunits, 
 (ii) wherein at least one of the plurality of native or endogenously-expressed receptor subunits has been engineered or modified to include an FcγRI receptor component, and 
 (iii) wherein the FcγRI receptor component is operative to bind to a target detector molecule that binds to or otherwise interacts with a predetermined target. 
   
     
     
         22 . The programmable immunocyte receptor complex cell system of  claim 21 , wherein the plurality of native or endogenously-expressed receptor subunits are T cell receptor subunits. 
     
     
         23 . The programmable immunocyte receptor complex cell system of  claim 22 , wherein the plurality of native or endogenously-expressed receptor subunits includes at least one CD3-delta, CD3-gamma, TCR alpha, TCR beta, two CD3-zeta and two CD3-epsilon. 
     
     
         24 . The programmable immunocyte receptor complex cell system of  claim 23 , wherein the endogenously-expressed receptor subunit that has been engineered or modified to include a biotin-binding component is the CD3-epsilon subunit. 
     
     
         25 . The programmable immunocyte receptor complex cell system of  claim 21 , wherein the immunocyte is a CD4+ T cell, a CD8+ T cell, γδ T cell, or allogenic cell. 
     
     
         26 . The programmable immunocyte receptor complex cell system of  claim 21 , wherein the target detector molecule is an IgG antibody. 
     
     
         27 . The programmable immunocyte receptor complex cell system of  claim 21 , wherein the predetermined target is a cancer cell or cancer cell determinant of a known type, or an infectious disease agent or a determinant of an infectious disease agent of a known type. 
     
     
         28 . The programmable immunocyte receptor complex cell system of  claim 21 , wherein the system is adapted for diagnostic use. 
     
     
         29 . The programmable immunocyte receptor complex cell system of  claim 21 , wherein the system is adapted for therapeutic use.