Surface plasmon resonance sensor chip having sensor surface capable of capturing multiple species of antibodies and method of making

Abstract

A surface plasmon resonance chip includes a sensor surface having a substrate, an inert metal layer directly on the substrate, a surface layer directly on the inert metal layer, and fusion proteins immobilized on the surface layer. The fusion proteins can be selected from A/G fusion proteins, A/L fusion proteins, G/L fusion proteins and A/G/L fusion proteins.

1 . A surface plasmon resonance chip comprising a sensor surface, wherein the sensor surface comprises:
 a substrate;   an inert metal layer directly on the substrate;   a surface layer directly on the inert metal layer; and   fusion proteins immobilized on the surface layer.   
     
     
         2 . The surface plasmon resonance chip of  claim 1  wherein the substrate is a glass substrate. 
     
     
         3 . The surface plasmon resonance chip of  claim 1  wherein the inert metal layer is a layer comprising gold. 
     
     
         4 . The surface plasmon resonance chip of  claim 3  wherein the inert metal layer is a layer consisting essentially of gold. 
     
     
         5 . The surface plasmon resonance chip of  claim 1  wherein the surface layer is a carboxymethyl dextran layer. 
     
     
         6 . The surface plasmon resonance chip of  claim 1  wherein the fusion proteins are selected from the group consisting of A/G fusion proteins, A/L fusion proteins, G/L fusion proteins and A/G/L fusion proteins. 
     
     
         7 . The surface plasmon resonance chip of  claim 6  wherein the fusion proteins are A/G/L fusion proteins. 
     
     
         8 . A method of making a sensor surface comprising:
 providing a substrate;   depositing a metal layer onto a surface of the substrate;   depositing a surface layer onto a surface of the metal layer; and   immobilizing fusion proteins on the surface layer, wherein the fusion proteins are fusion proteins selected from the group consisting of A/G fusion proteins, A/L fusion proteins, G/L fusion proteins and A/G/L fusion proteins.   
     
     
         9 . The method of  claim 8  wherein the providing a substrate comprises providing a glass substrate. 
     
     
         10 . The method of  claim 8  wherein the depositing a metal layer onto a surface of the substrate comprises depositing a layer comprising gold onto a surface of the substrate. 
     
     
         11 . The method of  claim 10  wherein the layer comprising gold is a layer consisting essentially of gold. 
     
     
         12 . The method of  claim 8  wherein the depositing a surface layer onto a surface of the metal layer comprises depositing a carboxymethyl dextran layer onto a surface of the metal layer. 
     
     
         13 . The method of  claim 8  wherein the immobilizing fusion proteins on the surface layer comprises immobilizing A/G/L fusion proteins on the surface layer. 
     
     
         14 . The method of  claim 8  wherein the immobilizing fusion proteins on the surface layer comprises:
 applying a first surface treatment to the surface layer to creating active groups that are capable of attaching to the fusion proteins; 
 adding the fusion proteins to the surface layer and allowing the fusion proteins to attach to the active groups; and 
 applying a second surface treatment to the surface layer to deactivate the surface layer by blocking active groups that are not attached to the fusion proteins. 
 
     
     
         15 . The method of  claim 14  wherein the surface layer is a carboxymethyl dextran layer and the applying the first surface treatment comprises applying 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (“EDC”) and N-hy droxysuccinimide(“NHS”) to the carboxymethyl dextran layer to create active groups that are capable of attaching to the fusion proteins. 
     
     
         16 . The method of  claim 15  wherein the adding the fusion proteins to the surface layer and allowing the fusion proteins to attach to the active groups comprises adding the fusion proteins at a pH of less than 4, such as a pH of 3. 
     
     
         17 . The method of  claim 16  wherein the applying a second surface treatment comprises applying ethanolamine to the carboxymethyl dextran layer to deactivate the surface layer by blocking active groups that are not attached to the fusion proteins. 
     
     
         18 . The method of  claim 8  wherein the fusion proteins are A/G/L fusion proteins. 
     
     
         19 . A method of using a surface plasmon resonance sensor chip to capture any species of antibody, comprising:
 providing a surface plasmon resonance chip comprising a sensor surface, wherein the sensor surface comprises: a substrate, an inert metal layer directly on the substrate, a surface layer directly on the inert metal layer, and A/G/L fusion proteins immobilized on the surface layer; and   contacting any species of antibody with the A/G/L fusion proteins to allow the A/G/L fusion proteins to capture the antibody.   
     
     
         20 . The method of  claim 19  wherein the antibody is selected from the group consisting of human, mouse, rat cow, goat, sheep, rabbit, guinea pig, pig, dog and cat IgG. 
     
     
         21 . The method of  claim 19  wherein the sensor surface comprises a glass substrate, a gold metal layer directly on the glass substrate, a carboxymethyl dextran layer directly on the gold metal layer, and A/G/L fusion proteins immobilized on the carboxymethyl dextran layer.