A Composition Containing SMAD Protein for Treatment of Autoimmune Diseases, a Fusion Protein Comprising SMAD Protein, an Expression Vector and a Method for Preparing the Same

Abstract

The present invention relates to a composition and a fusion protein for prevention or treatment of autoimmune diseases, which contain a Smad protein, and provides a method for prevention or treatment of autoimmune diseases, including lupus nephritis and rheumatoid arthritis.

1 .- 25 . (canceled) 
     
     
         26 . A fusion protein comprising: a Smad protein or a Smad transcription modulation domain protein; and a protein transduction domain, wherein the Smad is Smad6 or Smad7. 
     
     
         27 . The fusion protein of  claim 26 , wherein the protein transduction domain is one or more selected from the group consisting of Hph-1, Sim-2, Tat, VP22, Antp (antennapedia), Pep-1 (peptide-1), PTO-5 (protein transduction domain-5), 7R, 9R, 11R, and CTP (cytoplasmic transduction peptide). 
     
     
         28 . The fusion protein of  claim 26 , wherein the protein transduction domain comprises an amino acid sequence of SEQ ID NO: 5. 
     
     
         29 . The fusion protein of  claim 26 , wherein the protein transduction domain is encoded by a nucleic acid sequence of SEQ ID NO: 6. 
     
     
         30 . The fusion protein of  claim 26 , wherein the autoimmune disease is one or more selected from the group consisting of systemic lupus erythematosus, rheumatoid arthritis, septic shock, allergic asthma, allergic nasitis, atopic dermatitis, ulcerative colitis, dacryoadenitis, Alzheimer's disease, stroke, arteriosclerosis, vascular restenosis, type I diabetes, type II diabetes, urticaria, conjunctivitis, psoriasis, systemic inflammatory response syndrome, polymyositis, dermatomyositis, polyarthritis nodosa, mixed connective tissue disease, Sjogren's syndrome, gout, Parkinson's disease, amyotrophic lateral sclerosis, diabetic retinopathy, multiple sclerosis, Crohn's disease, chronic thyroiditis, Celiac disease, myasthenia gravis, pemphigus vulgaris, viral diseases, bacterial diseases, radiation-induced disorders, arteriosclerosis, hemangioma, angiofibroma, reperfusion injury, and cardiac hypertrophy. 
     
     
         31 . A composition for prevention or treatment of an autoimmune disease, comprising the fusion protein of  claim 26  as an active ingredient. 
     
     
         32 . A method for prevention or treatment of an autoimmune disease, comprising: administering the fusion protein of  claim 26  as an active ingredient to a subject in need. 
     
     
         33 . The method of  claim 32 , wherein the protein transduction domain is one or more selected from the group consisting of Hph-1, Sim-2, Tat, VP22, Antp (antennapedia), Pep-1 (peptide-1), PTO-5 (protein transduction domain-5), 7R, 9R, 11R, and CTP (cytoplasmic transduction peptide). 
     
     
         34 . The method of  claim 32 , wherein the protein transduction domain comprises an amino acid sequence of SEQ ID NO: 5. 
     
     
         35 . The method of  claim 32 , wherein the protein transduction domain is encoded by a nucleic acid sequence of SEQ ID NO: 6. 
     
     
         36 . The method of  claim 32 , wherein the autoimmune disease is one or more selected from the group consisting of systemic lupus erythematosus, rheumatoid arthritis, septic shock, allergic asthma, allergic nasitis, atopic dermatitis, ulcerative colitis, dacryoadenitis, Alzheimer's disease, stroke, arteriosclerosis, vascular restenosis, type I diabetes, type II diabetes, urticaria, conjunctivitis, psoriasis, systemic inflammatory response syndrome, polymyositis, dermatomyositis, polyarthritis nodosa, mixed connective tissue disease, Sjogren's syndrome, gout, Parkinson's disease, amyotrophic lateral sclerosis, diabetic retinopathy, multiple sclerosis, Crohn's disease, chronic thyroiditis, Celiac disease, myasthenia gravis, pemphigus vulgaris, viral diseases, bacterial diseases, radiation-induced disorders, arteriosclerosis, hemangioma, angiofibroma, reperfusion injury, and cardiac hypertrophy.