Contact-type patch, staining method using the same, and manufacturing method thereof

Abstract

The present disclosure relates to a gel-phase patch that performs a function of assisting in staining during a staining process such as a process of coming into contact with a specimen such as blood to perform a staining function of staining the specimen, a process of fixing the specimen, or a process of forming an optimal pH at a specimen stained by a staining sample. According to an aspect of the present disclosure, a contact-type staining patch includes a staining solution that reacts with a specimen and a gel receptor provided as a gel matrix of a mesh structure in which a pore that accommodates the staining solution is formed and the mesh structure prevents the staining solution in the pore from leaking or degenerating, and having a contact surface that comes into contact with the specimen to transfer some of the staining solution to the specimen.

What is claimed is: 
     
         1 . A contact-type staining patch comprising:
 a staining solution configured to react with a specimen; and   a gel receptor provided as a gel matrix of a mesh structure in which a pore that accommodates the staining solution is formed and the mesh structure prevents the staining solution in the pore from leaking or degenerating, and having a contact surface that comes into contact with the specimen to transfer some of the staining solution to the specimen.   
     
     
         2 . The contact-type staining patch of  claim 1 , wherein:
 the staining solution includes a staining sample configured to react with the specimen and a solvent configured to create a reaction condition of the staining sample; and   the gel receptor uses the mesh structure to maintain a reaction condition of the staining solution.   
     
     
         3 . The contact-type staining patch of  claim 1 , wherein, in a process in which the staining solution is transferred to the specimen through the contact surface, the gel receptor inhibits excessive movement of the staining solution by using the mesh structure to prevent residue from remaining at the specimen. 
     
     
         4 . The contact-type staining patch of  claim 1 , wherein the contact-type staining patch is a staining patch configured to assign a color to a specific component of the specimen, an antibody patch including an antibody that causes an antigen-antibody reaction with the specimen, or a DNA patch including a DNA probe that couples to a specific DNA sequence of the specimen. 
     
     
         5 . The contact-type staining patch of  claim 1 , wherein the staining solution includes a detection inducing substance selected from a staining substance that directly stains the specimen, a precipitation/aggregation inducing substance that either precipitates or aggregates the specimen, a fluorescent substance that allows the specimen to form a fluorescent color, an isotope that allows the specimen to be detected with radiation, and an enzyme attached to the specimen which secretes a detectable substance. 
     
     
         6 . The contact-type staining patch of  claim 5 , wherein:
 the staining solution includes an attaching substance that is coupled to the detection inducing substance and reacts with a specific component of the specimen to be attached to the specimen; and   the attaching substance includes an antibody that causes an antigen-antibody reaction with the specimen or a DNA probe that has a sequence complementary to a specific DNA sequence of the specimen.   
     
     
         7 . The contact-type staining patch of  claim 1 , wherein:
 the staining solution is provided in a liquid state; and   the gel receptor is provided as hydrogel that inhibits a leakage of the staining solution in the liquid state to the outside.   
     
     
         8 . A contact-type staining patch comprising:
 a gel receptor provided as a gel matrix of a mesh structure in which a pore is formed and in which any one surface is a contact surface that comes into contact with a specimen; and   a staining solution accommodated in the pore and configured to include a staining sample that reacts with the specimen and a buffering solution having a predetermined pH value to form an optimal pH when a reaction occurs between the specimen and the staining sample,   wherein the mesh structure inhibits a leakage to an outside or contamination of the staining solution and stores the staining solution in the gel receptor while maintaining the predetermined pH value of the staining solution, and, when the contact surface comes into contact with the specimen, allows some of the staining solution to move to the specimen and stain the specimen.   
     
     
         9 . The contact-type staining patch of  claim 8 , wherein the predetermined pH value is the optimal pH or a pH that has a difference of a pH compensation value from the optimal pH. 
     
     
         10 . The contact-type staining patch of  claim 9 , wherein a size of the predetermined pH value is within a pH range of 0.1 to 0.4. 
     
     
         11 . The contact-type staining patch of  claim 9 , wherein the pH compensation value is determined according to at least one of a hardness and porosity of the gel receptor, a density of the mesh structure, and a gel concentration of the gel receptor. 
     
     
         12 . The contact-type staining patch of  claim 11 , wherein the pH compensation value increases as the gel receptor is harder, increases as the porosity is smaller, increases as the density is higher, and increases as the gel concentration increases. 
     
     
         13 . A contact-type staining patch comprising:
 a staining solution that includes a first staining sample that stains a specimen and a second staining sample that stains the specimen and is different from the first staining sample; and   a gel receptor provided as a gel matrix that forms a pore configured to accommodate the staining solution and store the staining solution accommodated in the pore, and configured to come into contact with the specimen to transfer the first staining sample and the second staining sample to the specimen.   
     
     
         14 . A method of staining a specimen using a contact-type staining patch the includes a staining solution configured to react with the specimen, and a gel receptor provided as a gel matrix of a mesh structure in which a pore that accommodates the staining solution is formed and the mesh structure prevents the staining solution in the pore from leaking or degenerating, and having a contact surface that comes into contact with the specimen to transfer some of the staining solution to the specimen, the method comprising:
 preparing the contact-type staining patch;   contacting the specimen with the contact surface of the contact-type staining patch; and   staining the specimen by the contact-type staining patch.   
     
     
         15 . The method of  claim 14 , wherein, in the staining, the gel receptor maintains the staining solution in a state in which a reaction condition of the specimen is satisfied. 
     
     
         16 . The method of  claim 14 , further comprising, after the staining, omitting processes of washing and drying the specimen and observing the stained specimen.