Uses of novel fatty acid desaturases and elongases and products thereof

Abstract

The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturases and elongases from the organism Emiliana huxleyi . The invention also provides recombinant expression vectors containing desaturase or elongase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g. arachidonic acid (ARA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA).

We claim: 
     
         1 . Plant seed oil obtained from seeds of a transgenic plant comprising docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and docosapentaenoic acid (DPA), wherein the oil comprises at least 1% DHA and not more than 6% EPA, based on the total fatty acids of the transgenic seeds, and wherein the oil has a ratio of DHA to DPA of at least 2. 
     
     
         2 . The oil of  claim 1 , wherein the transgenic plant is a transgenic oilseed plant. 
     
     
         3 . The oil of  claim 2 , wherein the oilseed plant is flax ( Linum  sp.), rapeseed ( Brassica  sp.), soybean ( Glycine  sp.), sunflower ( Helianthus  sp.), cotton ( Gossypium  sp.), corn ( Zea mays ), olive ( Olea  sp.), safflower ( Carthamus  sp.), cocoa ( Theobroma cacoa ), peanut ( Arachis  sp.), hemp, camelina,  crambe , oil palm, coconuts, groundnuts, sesame, castor bean,  lesquerella , tallow tree, sheanuts, tungnuts, kapok fruit, poppy, jojoba, or  perilla.    
     
     
         4 . The oil of  claim 2 , wherein the oilseed plant is from the plant family of Brassicaceae. 
     
     
         5 . The oil of  claim 2 , wherein the oilseed plant is a  Brassica  plant. 
     
     
         6 . The oil of  claim 1 , wherein the oil has a ratio of DHA to DPA of 2.9. 
     
     
         7 . The oil of  claim 1 , wherein the oil comprises at least 1% but not more than 5% EPA. 
     
     
         8 . The oil of  claim 1 , wherein the DHA, EPA and DPA are triglyceride esters. 
     
     
         9 . Plant seed oil obtained from seeds of a transgenic plant plant comprising docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and docosapentaenoic acid (DPA), wherein the oil comprises at least 1% DHA and not more than 6% EPA, based on the total fatty acids of the transgenic seed, and wherein the seeds of the transgenic plant have a conversion rate of DPA to DHA of at least 75%. 
     
     
         10 . The oil of  claim 9 , wherein the transgenic plant is a transgenic oilseed plant. 
     
     
         11 . The oil of  claim 10 , wherein the oilseed plant is flax ( Linum  sp.), rapeseed ( Brassica  sp.), soybean ( Glycine  sp.), sunflower ( Helianthus  sp.), cotton ( Gossypium  sp.), corn ( Zea mays ), olive ( Olea  sp.), safflower ( Carthamus  sp.), cocoa ( Theobroma cacoa ), peanut ( Arachis  sp.), hemp, camelina,  crambe , oil palm, coconuts, groundnuts, sesame, castor bean,  lesquerella , tallow tree, sheanuts, tungnuts, kapok fruit, poppy, jojoba, or  perilla.    
     
     
         12 . The oil of  claim 10 , wherein the oilseed plant is from the plant family of Brassicaceae. 
     
     
         13 . The oil of  claim 10 , wherein the oilseed plant is a  Brassica  plant. 
     
     
         14 . The oil of  claim 9 , wherein the oil has a ratio of DHA to DPA of at least 2. 
     
     
         15 . The oil of  claim 14 , wherein the ratio of DHA to DPA is 2.9. 
     
     
         16 . The oil of  claim 9 , wherein the oil comprises not more than 5% EPA. 
     
     
         17 . The oil of  claim 9 , wherein the DHA, EPA and DPA are triglyceride esters.