Modular Fluid Dispensing Devices

Abstract

The present disclosure provides devices which deliver fluids from several reservoirs to a reaction vessel and eventually to a waste chamber in a predetermined schedule. The device provides improved simplicity while improving operational robustness and flexibility.

What is claimed is: 
     
         1 . A liquid dispensing device comprising:
 a wash buffer reservoir and at least one reagent reservoir, each reservoir comprising a reservoir control valve, wherein each reservoir control valve is in electrical communication with an electronic control board;   a reaction vessel in fluid communication with the wash buffer reservoir and the at least one reagent reservoir, wherein the reaction vessel comprises one or more baffles;   a waste tray in fluid communication with the reaction vessel;   a motorized cam in physical connection with the reaction vessel; and   an optional central pump.   
     
     
         2 . The device of  claim 1 , wherein each of the wash buffer reservoirs and at least one reagent reservoir comprise a port which is in fluid communication with a first port in the reaction vessel, and wherein the device further comprises a central pump. 
     
     
         3 . The device of  claim 2 , wherein each of the port of the wash buffer reservoir and the port of the at least one reagent reservoir are in fluid communication by tubing with the first port of the reaction vessel. 
     
     
         4 . The device of  claim 2 , wherein each reservoir control valve either controls the ports of the wash buffer reservoir and the at least one reagent reservoir, or controls the fluid communication within the respective tubing. 
     
     
         5 . The device of  claim 3 , wherein the tubing from the wash buffer reservoir and the tubing from the at least one reagent reservoir are in fluid communication with a multi-way connector and the central pump. 
     
     
         6 . The device of  claim 5 , wherein the multi-way connector is in fluid communication with the reaction vessel by tubing. 
     
     
         7 . The device of  claim 1 , wherein a port in the waste tray is in fluid communication with a second port in the reaction vessel by tubing. 
     
     
         8 . The device of  claim 7 , wherein a reaction vessel control valve either controls the second port of the reaction vessel, or controls the fluid communication within the tubing from the reaction vessel to the waste tray. 
     
     
         9 . The device of  claim 1 , wherein the electronic control board controls the opening and closing of each control valve, turns the central pump on and off, and controls the length of time that each control valve is opened. 
     
     
         10 . The device of  claim 9 , wherein the electronic control board contains software that is programmable to control the sequence of control valve opening and closing and the length of time each control valve is opened. 
     
     
         11 . The device of  claim 1 , wherein the electronic control board is programmable to control the speed of the motorized cam. 
     
     
         12 . The device of  claim 1 , wherein the reaction vessel is tethered at one end and is positioned to rock up and down at the distal end by interaction with the cam. 
     
     
         13 . The device of  claim 1 , wherein the cam comprises an off-set shape. 
     
     
         14 . The device of  claim 1 , further comprising a communication port for ISO9000 type compliance. 
     
     
         15 . The device of  claim 1 , wherein the electronic control board comprises Bluetooth remote control capability. 
     
     
         16 . The device of  claim 1 , wherein the reaction vessel is asymmetric in regard to the second port and axis of rotation of the reaction vessel. 
     
     
         17 . The device of  claim 1 , wherein the reaction vessel further comprises a cylinder that moves up and down with the action of the cam. 
     
     
         18 . The device of  claim 1 , wherein the reaction vessel comprises a plurality of analysis sections. 
     
     
         19 . The device of  claim 1 , wherein the entire device is less than 30 cm×50 cm, less than 20 cm×30 cm, or less than 16 cm×24 cm, and weighs less than 10 kg, or less than 5 kg, or less than 2 kg. 
     
     
         20 . The device of  claim 1 , wherein each of the tubing is about ⅛ inch internal diameter and about 3/16 inch outside diameter. 
     
     
         21 . The device of  claim 1 , wherein each of the at least one reagent reservoirs comprises an interior capable of holding up to 5 ml, up to 10 ml, or up to 20 ml of reagent. 
     
     
         22 . The device of  claim 1 , wherein a chassis connects each of the wash buffer reservoir, reagent reservoir(s), reaction vessel, wash tray, and cam motor and is also the exterior shell of the device. 
     
     
         23 . A method of analyzing a membrane using the device of  claim 1  comprising:
 loading a wash buffer into the wash buffer reservoir; 
 loading a first reagent into one of the at least one reagent reservoirs; 
 optionally loading a second reagent into a different one of the at least one reagent reservoirs; 
 loading the membrane and blocking reagent into the reaction vessel; 
 selecting a routine from the electronic control board; and 
 removing the membrane from the reaction vessel for subsequent signal development and result acquisition.