Composition Containing SMAD Protein for Treatment of Autoimmune Diseases, a Fusion Protein Comprising SMAD Protein, an Expression Vector and a Method for Preparing the Same

Abstract

The present invention relates to a composition and a fusion protein for prevention or treatment of autoimmune diseases, which contain a Smad protein, and provides a method for prevention or treatment of autoimmune diseases, including lupus nephritis and rheumatoid arthritis.

1 .- 25 . (canceled) 
     
     
         26 . An expression vector comprising a nucleic acid sequence that encodes the fusion protein comprising a Smad3 protein or a Smad3 transcription modulation domain protein; and a protein transduction domain, wherein Smad3 protein comprises an amino acid sequence of SEQ ID NO: 1 and the Smad3 transcription modulation domain protein comprises an amino acid sequence of SEQ ID NO: 3. 
     
     
         27 . A recombinant host cell overexpressing an exogenous nucleic acid sequence that encodes the fusion protein comprising a Smad3 protein or a Smad3 transcription modulation domain protein; and a protein transduction domain, wherein Smad3 protein comprises an amino acid sequence of SEQ ID NO: 1 and the Smad3 transcription modulation domain protein comprises an amino acid sequence of SEQ ID NO: 3. 
     
     
         28 . A host cell transformed with the expression vector of  claim 26 . 
     
     
         29 . The recombinant host cell of  claim 27 , which is a microbial cell, an animal cell, a plant cell, a cultured cell of animal origin, or a cultured cell of plant origin. 
     
     
         30 . The host cell of  claim 28 , which is a microbial cell, an animal cell, a plant cell, a cultured cell of animal origin, or a cultured cell of plant origin. 
     
     
         31 . A method for producing a fusion protein, comprising the steps of:
 transforming a host cell with the expression vector of  claim 26 ; and   culturing the host cell to express the fusion protein.   
     
     
         32 . A method for treating an autoimmune disease, comprising:
 administering a fusion protein comprising: a Smad3 protein or a Smad3 transcription modulation domain protein; and a protein transduction domain, wherein Smad3 protein comprises an amino acid sequence of SEQ ID NO: 1 and the Smad3 transcription modulation domain protein comprises an amino acid sequence of SEQ ID NO: 3 to a subject in need.   
     
     
         33 . The method of  claim 32 , wherein the Smad3 protein is encoded by a nucleic acid sequence of SEQ ID NO: 2, and the Smad3 transcription modulation domain protein is encoded by a nucleic acid sequence of SEQ ID NO: 4. 
     
     
         34 . The method of  claim 32 , wherein the protein transduction domain is one or more selected from the group consisting of Hph-1, Sim-2, Tat, VP22, Antp (antennapedia), Pep-1 (peptide-1), PTO-5 (protein transduction domain-5), 7R, 9R, 11 R, and CTP (cytoplasmic transduction peptide). 
     
     
         35 . The method of  claim 32 , wherein the protein transduction domain comprises an amino acid sequence of SEQ ID NO: 5. 
     
     
         36 . The method of  claim 32 , wherein the protein transduction domain is encoded by a nucleic acid sequence of SEQ ID NO: 6. 
     
     
         37 . The method of  claim 32 , wherein the fusion protein comprises an amino acid sequence of SEQ ID NO: 7 or 9. 
     
     
         38 . The method of  claim 32 , wherein the fusion protein is encoded by a nucleic acid sequence of SEQ ID NO: 8 or 10. 
     
     
         39 . The method of  claim 32 , wherein the autoimmune disease is one or more selected from the group consisting of systemic lupus erythematosus, rheumatoid arthritis, septic shock, allergic asthma, allergic nasitis, atopic dermatitis, ulcerative colitis, dacryoadenitis, Alzheimer's disease, stroke, arteriosclerosis, vascular restenosis, type I diabetes, type II diabetes, urticaria, conjunctivitis, psoriasis, systemic inflammatory response syndrome, polymyositis, dermatomyositis, polyarthritis nodosa, mixed connective tissue disease, Sjogren's syndrome, gout, Parkinson's disease, amyotrophic lateral sclerosis, diabetic retinopathy, multiple sclerosis, Crohn's disease, chronic thyroiditis, Celiac disease, myasthenia gravis, pemphigus vulgaris, viral diseases, bacterial diseases, radiation-induced disorders, arteriosclerosis, hemangioma, angiofibroma, reperfusion injury, and cardiac hypertrophy.